Validated HPLC–MS/MS Method for Simultaneous Determination of Curcumin and Piperine in Human Plasma

Wang, Xiumei; Zhang, Qizhi; Yang, Jin; Zhu, Rong; Zhang, Jun; Cai, Li-Jing; Peng, Wei

doi:10.4314/tjpr.v11i4.13

Cited by 23 publications

(18 citation statements)

References 16 publications

(16 reference statements)

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“…Piperine is hydrophobic in nature with a calculated logP value of 3.38, and thus a reversed phase column was considered for its analyses. There are many reported RP‐HPLC and LC‐MS methods for bioanalysis of piperine, employing mobile phases composed of acetonitrile as an organic modifier and an aqueous phase composing of trifluroacetic acid, ammonium acetate, or formic acid at low pH. The same were considered for the development and optimization of UHPLC‐HRMS method in this study for the separation of piperine and its metabolites.…”

Section: Methodsmentioning

confidence: 99%

Characterization of stable and reactive metabolites of piperine formed on incubation with human liver microsomes

et al. 2019

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Black pepper, though commonly employed as a spice, has many medicinal properties.It consists of volatile oils, alkaloids, pungent resins, etc., of which piperine is a major constituent. Though safe at low doses, piperine causes alteration in the activity of drug metabolising enzymes and transporters at high dose and is known to precipitate liver toxicity. It has a potential to form reactive metabolite(s) (RM) owing to the presence of structural alerts, such as methylenedioxyphenyl (MDP), α, β-unsaturated carbonyl group (Michael acceptor), and piperidine. The present study was designed to detect and characterize stable and RM(s) of piperine formed on in vitro incubation with human liver microsomes. The investigation of RMs was done with the aid of trapping agents, viz, glutathione (GSH) and N-acetylcysteine (NAC). The samples were analysed by ultra-high performance liquid chromatography coupled with high resolution mass spectrometry (UHPLC-HRMS) using Thermo Scientific Q Exactive Plus Orbitrap. Full scan MS followed by data-dependent MS 2 (Full MS-ddMS 2 ) mode was used to establish mass spectrometric fragmentation pathways of protonated piperine and its metabolites. In total, four stable metabolites and their isomers (M1a-c, M2a-b, M3a-c, and M4a-b) were detected. Their formation involved removal of carbon (3, M1a-c), hydroxylation (2, M2a-b), hydroxylation with hydrogenation (3, M3a-c), and dehydrogenation (2, M4a-b). Out of these metabolites, M1, M2, and M3 are reported earlier in the literature, but their isomers and two M4 variants are novel. In addition, six novel conjugates of RMs, including three GSH conjugates of m/z 579 and three NAC conjugates of m/z 435, were also observed.

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Section: Methodsmentioning

confidence: 99%

Characterization of stable and reactive metabolites of piperine formed on incubation with human liver microsomes

et al. 2019

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“…Hasil spektrogram MS dari puncak 3 menunjukkan waktu retensi pada 3,4 menit dan harga berat molekul senyawa alkaloid [M+H] + sebesar 286,2811g/mol. Berat molekul yang diperoleh diduga merupakan senyawa piperin, hal ini diperkuat berdasarkan penelitian [8] menggunakan metode LC-MS yang memperoleh berat molekul dari piperin adalah sebesar 286,3 g/mol.…”

Section: Analisis Isolatunclassified

“…Berdasarkan data-data yang diperoleh dari hasil analisis dan sesuai dengan penelitian [6][7][8], diduga isolat alkaloid yang telah diisolasi dari rimpang lengkuas merah mengandung senyawa piperin (C17H19NO3). Struktur molekul dari piperin ditunjukkan pada Gambar 8.…”

Section: Analisis Isolatunclassified

Isolasi dan Identifikasi Senyawa Golongan Alkaloid dari Rimpang Lengkuas Merah (Alpinia purpurata)

2016

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Telah dilakukan penelitian tentang Isolasi dan Identifikasi Senyawa Golongan Alkaloid dari Rimpang Lengkuas Merah (Alpinia purpurata). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa golongan alkaloid dari ekstrak rimpang lengkuas merah (Alpinia purpurata). Penapisan fitokimia simplisia kering diketahui bahwa terdapat senyawa golongan alkaloid, flavonoid, saponin, dan steroid. Hasil karakterisasi dengan menggunakan Spektrofotometer UV-Vis menghasilkan panjang gelombang maksimal pada 338 nm. Analisis menggunakan FTIR terdapat gugus C-N, C=O amida, C=C aromatik, C-O eter, dan C-H alifatik. Analisis menggunakan LC-MS diperoleh berat molekul sebesar 286,28 g/mol [M+H]+. Berdasarkan data-data dari hasil tersebut diduga dalam isolat murni mengandung senyawa piperin (C17H19NO3).

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“…Methods routinely used in pharmaceutical analysis are liquid chromatography (LC) or gas chromatography (GC) as separation techniques or coupled with ultraviolet-visible (UV-VIS), mass spectrometry (MS) or nuclear magnetic resonance (NMR) detection [1,2]. There are, however, alternative methodologies with significant potential that are economically attractive and environmentally friendly.…”

Section: Introductionmentioning

confidence: 99%

An underestimated technique. Does pressurized and pressure-assisted capillary electrochromatography have potential in drug and pharmacological-active compounds analysis?

Trzebiatowski

Gwarda

Dzido

2019

Current Issues in Pharmacy and Medical Sciences

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Pharmaceutical analysis guarantees patient safety all over the world. Thus, continuous development of existing analytical techniques is still very important. Pressurized capillary electrochromatography and pressure-assisted capillary electrochromatography are hybrid separation techniques that combine the selectivity of liquid chromatography and the high separation efficiency of capillary electrophoresis. They use a smaller amount of reagents and samples, hence, reducing the total cost of analysis. Therefore they have found application in a number of pharmaceutical and biomedical analysis. This review article focuses on the use and importance of pressurized and pressure-assisted capillary electrochromatography in pharmaceutical and biomedical analysis, taking into account types of detectors and capillaries used. Despite the fact that pressurized capillary electrochromatography and pressure assisted capillary electrochromatography offer many possibilities and have been available for over a dozen years, they are still underdevelopment and not fully explored.

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Validated HPLC–MS/MS Method for Simultaneous Determination of Curcumin and Piperine in Human Plasma

Cited by 23 publications

References 16 publications

Characterization of stable and reactive metabolites of piperine formed on incubation with human liver microsomes

Characterization of stable and reactive metabolites of piperine formed on incubation with human liver microsomes

Isolasi dan Identifikasi Senyawa Golongan Alkaloid dari Rimpang Lengkuas Merah (Alpinia purpurata)

An underestimated technique. Does pressurized and pressure-assisted capillary electrochromatography have potential in drug and pharmacological-active compounds analysis?

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