Seminal plasma reduces exogenous oxidative damage to human sperm, determined by the measurement of DNA strand breaks and lipid peroxidation

Potts, Ryan; Notarianni, L. J.; Jefferies, T.M.

doi:10.1016/s0027-5107(99)00215-8

Cited by 126 publications

(71 citation statements)

References 26 publications

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“…Pathogenic bacteria such as streptococci, staphylococci, Mycoplasma, Chlamydia and Ureaplasma produce an acute inflammatory response with a flow of leucocytes into the genital tract leading to increase the level of reactive oxygen species (ROS) production (29,(40)(41)(42). Excessive like these substances have negative effects on sperm parameters (43).…”

Section: Male Genital Tract Infection and Bacteriospermiamentioning

confidence: 99%

The Impact of Bacterial Infections on Human Spermatozoa

Zeyad¹,

Amor²,

Hammadeh³

2017

International Journal of Women’s Health and Reproduction Scienc

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Section: Male Genital Tract Infection and Bacteriospermiamentioning

confidence: 99%

The Impact of Bacterial Infections on Human Spermatozoa

Zeyad¹,

Amor²,

Hammadeh³

2017

International Journal of Women’s Health and Reproduction Scienc

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“…The enzymatic antioxidants include the following components: superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT). These enzymes are constitutively expressed in human semen [1,27]. The free radical scavenging activity of SOD, GPX and CAT protects the sperm membrane against the LPO, preserving its integrity and hence maintaining the normal sperm parameters of motility and morphology [6,7].…”

Section: Introductionmentioning

confidence: 99%

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Atig

Kerkeni

Saâd

et al. 2013

J Assist Reprod Genet

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Purpose To evaluate levels of sperm DNA fragmentation and enzymatic antioxidant status in seminal plasma of Tunisian fertile and infertile men in order to assess the effects of seminal oxidative stress on sperm DNA integrity and semen quality. Methods Semen samples from 100 infertile patients (40 oligoasthenoteratozoospermics, 31 teratozoospermics and 29 asthenozoospermics) and 50 fertile men (controls) were analyzed for DNA fragmentation by TUNEL assay and biochemical parameters. Seminal antioxidant activities (Superoxide dismutase, Glutathione peroxidase and Catalase) and malondialdehyde concentrations were measured spectrophotometrically.Results Sperm DNA fragmentation and malondialdehyde levels in infertile groups were more elevated than controls. Nevertheless, the activities of the antioxidant enzymes were significantly lower in abnormal groups compared to normozoospermics. Sperm DNA fragmentation was closely and positively correlated to malondialdehyde levels (r=0.37, P=0.008); meanwhile, reduced seminal antioxidant profile was negatively associated to sperm DNA fragmentation. Interestingly, we noted also that sperm DNA fragmentation was negatively correlated to sperm motility (r=−0.54, P<0.001) and positively associated to the abnormal sperm morphology (r=0.57, P=0.002). Conclusions This report revealed that increased sperm DNA fragmentation can be due to the impaired seminal enzymatic antioxidant profile and increased Lipid peroxidation. Our results sustain that the evaluation of sperm DNA fragmentation and seminal oxidative biomarkers in infertile men is recommended as a consistent prognostic tool for male infertility assessment.

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“…The most important is the production of Reactive Oxygen Species (ROS), which is excited by excessive stress, competitive sports, alcohol and drug abuse or nicotine. If produced in abundance, ROS can enter the cell nucleus, bind to the DNA and cause its fragmentation [32][33][34][35]. Several studies have reported that redox balance is deregulated in the ejaculates from infertile males, with glutathione peroxidase 4 as one of the main enzymes involved in this issue [36].…”

Section: Discussionmentioning

confidence: 99%

Sperm DNA Fragmentation is Significantly Increased in Those Men with Morphologically Abnormal Spermatozoa

Ferreyra¹

2014

JFIV Reprod Med Genet

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Purpose: To evaluate the levels of DNA fragmentation regarding to spermatozoa morphology in male infertility patients attending Andrology Laboratory at fertility center.Methods: Semen samples of 196 patients were analyzed using computer-assisted semen analysis (CASA). Sperm DNA fragmentation was measured by the sperm chromatin dispersion test. According to the values of sperm morphology, the groups were conformed by teratozoospermic (105 patients) and normozoospermic (91 patients) men.Results: Subjects' ages ranged from 21 to 68 years. Teratozoospermic men were older that the normozoospermic men (39.72 ± 7.86 vs. 36.57 ± 6.29 years; P<0.05). Values of pH were similar in both evaluated groups (P:NS). Parameters of volume, concentration, motility and vitality in teratozoospermic men were significantly lower compared to those normozoospermic patients (P<0.05). High levels of DNA fragmentation were observed in those patients with abnormal sperm morphology compared to normozoospermic men (44.31 ± 7.52 vs. 34.92 ± 5.89; P<0.05). Conclusions:Men with abnormal spermatozoa morphology showed high levels of DNA fragmentation. These significantly high percentages of sperm DNA damage will be an additional factor that drastically reduces the possibility of success in these infertile men.

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Seminal plasma reduces exogenous oxidative damage to human sperm, determined by the measurement of DNA strand breaks and lipid peroxidation

Cited by 126 publications

References 26 publications

The Impact of Bacterial Infections on Human Spermatozoa

The Impact of Bacterial Infections on Human Spermatozoa

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Sperm DNA Fragmentation is Significantly Increased in Those Men with Morphologically Abnormal Spermatozoa

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