Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Atig, Fatma; Kerkeni, Abdelhamid; Saâd, Ali; Ajina, Mounir

doi:10.1007/s10815-013-9936-x

Cited by 37 publications

(39 citation statements)

References 39 publications

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“…For example our results are consistent with data gathered by Atig et al . () who observed an elevated DFI and decreased activity of SOD, catalase and Glutathione peroxidase, in a group of patients treated for infertility, compared with controls. Similarly, Tartibian et al .…”

Section: Discussionmentioning

confidence: 97%

Decreased activity of superoxide dismutase in the seminal plasma of infertile men correlates with increased sperm deoxyribonucleic acid fragmentation during the first hours after sperm donation

Wdowiak

Bakalczuk²,

Bakalczuk

2015

Andrology

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Sperm DNA fragmentation varies between individuals and is more pronounced with increased patient age and time after sperm donation. The intensification of DNA fragmentation depends on the balance of the oxidoreductive system, which is regulated mainly by two enzymes - superoxide dismutase (SOD) and catalase. The objective of this study was to determine the relationship between sperm DNA fragmentation dynamics, fertility and seminal SOD and catalase activity. The study was conducted in 2013 and 2014 at the Non-Public Health Care Unit 'Ovum Reproduction and Andrology' in Lublin, Lublin, Poland, and covered 218 men aged 25-35 (85 fertile and 133 patients treated for infertility). Percentage of fragmented DNA was measured in a modified chromatin dispersion test at four time points after sperm donation (t = 0, 3, 6, 12 h). SOD and catalase activities were determined spectrophotometrically. We confirmed that the activity of SOD in the seminal plasma of men with reproductive disorders was lower compared with fertile men. Conversely, no significant correlations were found between fertility and catalase activity. Sperm DNA of infertile males was initially more fragmented than fertile male sperm DNA. SOD and catalase activity did not correlate with the degree of DNA fragmentation in fertile men. In men with reproductive disorders, the rate of DNA fragmentation was slow within first 3 h after sperm donation and then increased between 6 and 12 h. In this group of infertile men, those with higher SOD activity had a lower DNA fragmentation index (DFI) after 12 h, and a reduced rate of intensity of fragmentation from 6 to 12 h. Alternatively, higher catalase activity among men treated for infertility was accompanied by higher initial DFI and higher rate of DNA fragmentation from 6 to 12 h. These results highlight the importance of determining a proper time window between sperm donation and procedures of assisted reproductive technology.

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Section: Discussionmentioning

confidence: 97%

Decreased activity of superoxide dismutase in the seminal plasma of infertile men correlates with increased sperm deoxyribonucleic acid fragmentation during the first hours after sperm donation

Wdowiak

Bakalczuk²,

Bakalczuk

2015

Andrology

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“…Oxidative stress occurs in results of oxidative imbalance by an increase in reactive oxygen species (ROS) or by decrease/absence in antioxidant status (Gulum et al, ; Riaz et al, ). This process can lead to damages to polyunsaturated lipids present in sperm plasma membrane, as well as to the DNA, RNA and proteins (Atig, Kerkeni, Saad, & Ajina, ; Kamkar, Ramezanali, & Sabbaghian, ; Mello Filho et al, ; Sposito et al, ). This imbalance can also be observed in reproductive biotechnologies, (e.g., semen cryopreservation; Amidi, Pazhohan, Shabani, Khodarahmian, & Nekoonam, ; Panner Selvam et al, ), in vitro fertilisation and in protocols with an excessive manipulation of semen, as plasma separation through centrifugation (Donnelly et al, ) and also sperm separation (Raad et al, ).…”

Section: Discussionmentioning

confidence: 99%

Carnosine treatment during human semen processing by discontinuous density gradient

et al. 2019

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The aim of this article was to evaluate the effects of different concentrations of carnosine added during human semen processing. Semen samples from 34 patients were submitted to processing by discontinuous density gradient centrifugation without (control) or with different concentrations of carnosine supplementation as follows: (a) 20 mM of carnosine supplementation on the layers of Percoll; and (b) 50 mM carnosine supplementation. Sperm samples were then washed with human tubal fluid medium and evaluated according to sperm kinetics and functional assessment. For statistical analysis, data were evaluated by a general linear model or a Friedman test, whenever appropriate. The 50 mM carnosine supplementation led to improved sperm mitochondrial activity when compared to untreated samples. Motility variables, such as percentage of motile and progressively motile spermatozoa, average path velocity, straight line velocity, curvilinear velocity and linearity, showed an improvement after semen processing irrespective of carnosine supplementation. Both concentrations of carnosine increased the beat‐cross frequency (BCF) when compared to samples before processing. We conclude that carnosine supplementation in semen samples benefits sperm mitochondrial activity and BCF.

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“…To be treated as morphologically normal, a spermatozoon would have a normal acrosome, an oval head between 2.5 and 3.5 μm width and 5 and 6 μm long, a midpiece of 4.0 to 5.0 μm, and a tail about 50 μm long 8 . Several reports have found higher rates of DNA damage, sperm aneuploidy, and protamination defects in infertile men with impaired semen parameters in comparison with controls and their association with morphological abnormalities 2,9‐12 …”

Section: Introductionmentioning

confidence: 99%

Teratozoospermia: Its association with sperm DNA defects, apoptotic alterations, and oxidative stress

2020

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This study was carried out to evaluate the level of nuclear sperm DNA damage in men with isolated polymorphic teratozoospermia and examining its relationship with apoptosis and oxidative stress. A total of 89 subjects were divided into two groups: men with isolated teratozoospermia (n = 69) and men with normal semen parameters (n = 20) as controls. Sperm DNA breaks were determined by using acridine orange staining. The proportion of viable spermatozoa with mitochondrial transmembrane depolarization was detected by fluorescence microscopy through the use of MitoPTJC‐1 staining method. Bivariate Annexin V/6‐CFDA analysis was then set out in order to measure the percentage of both viable and dead spermatozoa with phosphatidylserine (PS) externalization. Seminal antioxidant profile (reduced glutathione (GSHr); oxidized glutathione (GSSG); glutathione S‐transferase (GST)) and total protein sulfhydryl (P‐SH) concentrations were measured spectrophotometrically. Men with isolated teratozoospermia, when compared to controls, showed significantly increased level of single sperm DNA breaks and higher proportions of spermatozoa with phosphatidylserine externalization and mitochondrial depolarization. Among the differently studied oxidative stress seminal parameters, the rates of seminal GSHr, GST, and P‐SH were significantly decreased in the teratozoospermic group. However, the seminal rates of GSSG and GST have decreased, but only GST did not show a significant difference. Interestingly, significant correlations were found between the studied apoptotic markers and the rate of atypical sperm forms with the incidences of head abnormalities. Furthermore, positive inter‐correlations were found between sperm DNA defects, impaired seminal antioxidant status, and the apoptotic sperm markers. Such data provide clear evidence that the apoptotic alterations are closely correlated to abnormal sperm morphology and DNA damage. Moreover, decreased seminal antioxidant profile may be a crucial factor involved in the mechanism of sperm cell death‐mediated DNA breaks in teratozoospermic semen.

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Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Cited by 37 publications

References 39 publications

Decreased activity of superoxide dismutase in the seminal plasma of infertile men correlates with increased sperm deoxyribonucleic acid fragmentation during the first hours after sperm donation

Decreased activity of superoxide dismutase in the seminal plasma of infertile men correlates with increased sperm deoxyribonucleic acid fragmentation during the first hours after sperm donation

Carnosine treatment during human semen processing by discontinuous density gradient

Teratozoospermia: Its association with sperm DNA defects, apoptotic alterations, and oxidative stress

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