The Impact of Bacterial Infections on Human Spermatozoa

Zeyad, Ali; Amor, Houda; Hammadeh, Mohamad Eid

doi:10.15296/ijwhr.2017.43

Cited by 15 publications

(10 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regarding the analysis of sperm parameters, all samples were normozoospermic, independent if they had leucocytospermia or bacteriospermia. No relationship was found between leukocytosis and presence of bacteria, which has been evidenced in previous work that have recommended studying the aetiology of leucocytes even in the absence of seminal infection (Domes et al, ; Zeyad, Amor, & Eid Hammadeh, ).…”

Section: Discussionmentioning

confidence: 54%

Determination of leucocyte extracellular traps (ETs) in seminal fluid (ex vivo ) in infertile patients—A pilot study

et al. 2019

View full text Add to dashboard Cite

Leucocytospermia has been associated with loss of sperm function. Extracellular traps (ETs) of leucocytes are produced during innate immune response. ETs can be activated by spermatozoa in contact with polymorphonuclear (in vitro), inducing sperm entrapment and decrease motility. In this pilot study, we describe the results of ETosis ex vivo, in seminal fluid (SF) smear of infertile patients, associating ETs with leucocytospermia and bacteriospermia. In 21 infertile patients, semen parameters (WHO, 2010), microbiological study, leucocytospermia and presence of ETs in SF were determined. Leucocytes (CD45, CD15 and CD68) were evaluated by immunostaining in SF smears. Indirect immunofluorescence (global histone and H4‐citrullinated 3) and scanning electron microscopy (SEM) were used to determine ETs morphology. In 28.6% of patients presented leucocytospermia without bacteriospermia, all of them presented a large number of ETs in the SF smears examined. About 76.6% of the patients without leucocytospermia were positive for ETs. Samples with leucocytospermia have a higher number of ETs and would be related to the amount of leucocytes in the SF. The morphological predominant ETs were diffuse (diffETs) and spread (sprETs). The formation of ETs indicates leucocyte activation in semen, and it was observed that ETosis does not depend exclusively on the presence of bacterial contamination.

show abstract

Section: Discussionmentioning

confidence: 54%

Determination of leucocyte extracellular traps (ETs) in seminal fluid (ex vivo ) in infertile patients—A pilot study

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Increased ROS formation is strongly associated with the accumulation of JC-1 monomers in depolarized mitochondria [62]. Bacterial adhesion as well as soluble factors released by the bacteria may trigger rupture of the mitochondrial membrane [63]. Our JC-1 data particularly emphasize on the protective effects QUE may exhibit on the mitochondrial structures under bacteriospermia.…”

Section: Discussionmentioning

confidence: 99%

Antibiotics Versus Natural Biomolecules: The Case of In Vitro Induced Bacteriospermia by Enterococcus Faecalis in Rabbit Semen

et al. 2019

View full text Add to dashboard Cite

Male subfertility is a global issue in human reproduction as well as in animal reproduction. Bacterial infection and semen contamination are still widely overlooked. As the collection of ejaculates is not a sterile process, it is necessary to add antimicrobial agents to avoid a possible depreciation of semen samples. As traditionally used antibiotics have been questioned because of an ever-increasing bacterial resistance, natural bioactive molecules could offer an alternative because of their antibacterial and antioxidant properties. As such, we decided to compare the effects of selected natural biomolecules (resveratrol-RES, quercetin-QUE and curcumin-CUR) with routinely used antibiotics in animal biotechnologies (penicillin-PEN, gentamicin-GEN and kanamycin-KAN) on the rabbit sperm vitality in the presence of Enterococcus faecalis. Changes in the sperm structural integrity and functional activity were monitored at 0, 2, 4 and 6 h. Computer-assisted sperm analysis (CASA) was used for the assessment of spermatozoa motility. Production of reactive oxygen species (ROS) was evaluated using chemiluminiscence, while the mitochondrial membrane potential (ΔΨm) was examined using the JC-1 dye. Finally, the sperm chromatin dispersion (SCD) test was used to assess DNA fragmentation, and changes to the membrane integrity were evaluated with the help of annexin V/propidium iodide. The motility assessment revealed a significant sperm motility preservation following treatment with GEN (p < 0.001), followed by PEN and CUR (p < 0.01). QUE was the most capable substance to scavenge excessive ROS (p < 0.001) and to maintain ΔΨm (p < 0.01). The SCD assay revealed that the presence of bacteria and antibiotics significantly (p < 0.05) increased the DNA fragmentation. On the other hand, all bioactive compounds readily preserved the DNA integrity (p < 0.05). In contrast to the antibiotics, the natural biomolecules significantly maintained the sperm membrane integrity (p < 0.05). The microbiological analysis showed that GEN (p < 0.001), KAN (p < 0.001), PEN (p < 0.01) and CUR (p < 0.01) exhibited the strongest antibacterial activity against E. faecalis. In conclusion, all selected biomolecules provided protection to rabbit spermatozoa against deleterious changes to their structure and function as a result of Enterococcus faecalis contamination. Therefore, administration of RES, QUE and/or CUR to rabbit semen extenders in combination with a carefully selected antibacterial substance may be desirable.

show abstract

“…Infection with these bacteria has significant detrimental effects, not only on sperm parameters in general, but also specifically on DNA integrity (Zeyad, Amor, & Hammadeh, 2017).…”

Section: Chlamydia Trachomatismentioning

confidence: 99%

The role of infections and leukocytes in male infertility

2020

View full text Add to dashboard Cite

Oxidative stress is preempted by an adequate level of antioxidants, which scavenge oxidants when they are produced in excess by different sources, including leukocytes and immature spermatozoa. Enzymatic antioxidants, such as superoxide dismutase, catalase and glutathione peroxidase, and several nonenzymatic antioxidants (pro teins, vitamins and minerals), working as oxidant scavengers and cofactors of en zymatic antioxidants have been identified in seminal plasma. The total antioxidant capacity (TAC) is a diagnostic test that can be utilised in the male infertility workup. TAC measures the amount of total antioxidants in seminal plasma. Therefore, it pro vides an assessment of the reductive potential in seminal plasma. Several studies have investigated the diagnostic application of TAC in various andrology conditions. There is substantial evidence in the literature to show that infertile patients have lower seminal TAC in comparison with fertile men. Moreover, there is a positive cor relation between TAC and seminal parameters, such as sperm concentration, motility and morphology. Evaluation of TAC together with reactive oxygen species (ROS) and sperm DNA fragmentation index (DFI) may be beneficial in the diagnosis of male infertility.

show abstract

The Impact of Bacterial Infections on Human Spermatozoa

Cited by 15 publications

References 91 publications

Determination of leucocyte extracellular traps (ETs) in seminal fluid (ex vivo ) in infertile patients—A pilot study

Determination of leucocyte extracellular traps (ETs) in seminal fluid (ex vivo ) in infertile patients—A pilot study

Antibiotics Versus Natural Biomolecules: The Case of In Vitro Induced Bacteriospermia by Enterococcus Faecalis in Rabbit Semen

The role of infections and leukocytes in male infertility

Contact Info

Product

Resources

About