Semi-Quantitative Immunochromatographic Test for Prostate Specific Antigen in Whole Blood: Tossing the Coin to Predict Prostate Cancer?

Oberpenning, F.; Hetzel, Stefan; Weining, Christoph; Brandt, Burkhard; Angelis, G. De; Heinecke, Achim; Lein, Michael; Fornara, Paolo; Schmid, Hans‐Peter; Hertle, L.; Semjonow, Axel

doi:10.1016/s0302-2838(03)00095-2

Cited by 31 publications

(15 citation statements)

References 13 publications

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“…The increase of PSA levels above a cut-off value of 4.0 ng mL −1 is usually counted as positive and a biopsy might be needed [5]. When total PSA level was higher than a cut-off value of 10 ng mL −1 , it is generally suspected to be associated with PCa [6]. Therefore, the development of sensitive and specific methods for PSA is of great importance in the early diagnosis of PCa and monitoring of disease recurrence after treatment.…”

Section: Introductionmentioning

confidence: 99%

Detection of the human prostate-specific antigen using an aptasensor with gold nanoparticles encapsulated by graphitized mesoporous carbon

Liu

Hua

et al. 2012

Microchim Acta

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We report on a sensitive electrochemical aptasensor for the detection of human prostate specific antigen (PSA). It is based on the signal amplification of the biotin-avidin system using a sensing platform that is making use of a graphite electrode modified with gold nanoparticles that were covered with graphitized mesoporous carbon nanoparticles (AuNPs@GMCs). The AuNPs@GMCs hybrid was prepared by linking 1,6-hexanedithiol-functionalized GMCs and gold nanoparticles via Au-S groups. Then, streptavidin was immobilized on the electrode modified with the AuNPs@GMCs so to enlarge the amount of biotin-aptamer which led to enhanced detection sensitivity. If an PSA aptamer captures the target PSA on the electrode, the differential pulse voltammetric (DPV) signal of the hexacyanoferrate redox system decreases. Factors affecting the performance of the aptasensor were studied in detail. Under optimal conditions, the DPV signal changes could be used to quantitatively detect PSA in the concentration range from 0.25 to 200 ng mL −1 , with a lowest limit of detection as small as 0.25 ng mL −1 . The aptasensor is highly specific and displays acceptable precision, good stability and repeatability.

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Section: Introductionmentioning

confidence: 99%

Detection of the human prostate-specific antigen using an aptasensor with gold nanoparticles encapsulated by graphitized mesoporous carbon

Liu

Hua

et al. 2012

Microchim Acta

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“…The increase in prostate specific antigen (PSA) levels in serum above the normal limits (4 ng/mL) is the primary indication of prostate malignancy. An increased level of 10 ng/mL of the total PSA is often suspected to be associated with prostate cancer (Oberpenning et al, 2003). Consequently, PSA is usually used as a biomarker for the diagnosis and prognosis of prostate cancer.…”

Section: Introductionmentioning

confidence: 99%

Nanoporous gold film based immunosensor for label-free detection of cancer biomarker

Wei

Zhao

et al. 2011

Biosensors and Bioelectronics

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“…Total PSA (TPSA) is defined as the combination of both fPSA and ACT-PSA [8]. The cut-off limit of TPSA between prostate hyperplasia and cancer is 4 ng/mL (recently recommended as 2.5 ng/mL) [2,9].…”

Section: Introductionmentioning

confidence: 99%

Effect of biolinker on the detection of prostate specific antigen in an interferometry

Choi

Kim

2009

Biotechnol Bioproc E

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Prostate-specific antigen (PSA) has been identified as a significant biomarker for prostate cancer screening. Heavily-doped porous silicon, etched to form a Fabry-Perot fringe pattern, can be applied to an interferometric sensing for detecting PSA bound with PSA-antibody. In the previous works, a calyx crown derivative (Prolinker-A) was used as an alternative biolinker on the porous silicon surface for interferometric biosensing of DNA-damaging chemical instead of employing the conventional biomolecular affinity method using biotin, which resulted in a denser linker formation. In this study, G5 amineterminated PAMAM (poly amidoamine) dendrimer as a biolinker, was applied to an interferometric sensing of PSA by using porous silicon, which shows the enhancement of adhesion capability and increase of functional groups more than those with Prolinker-A. Considerably low level down to 1 ng/mL of PSA could be detected by this sensing system. © KSBB

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Semi-Quantitative Immunochromatographic Test for Prostate Specific Antigen in Whole Blood: Tossing the Coin to Predict Prostate Cancer?

Cited by 31 publications

References 13 publications

Detection of the human prostate-specific antigen using an aptasensor with gold nanoparticles encapsulated by graphitized mesoporous carbon

Detection of the human prostate-specific antigen using an aptasensor with gold nanoparticles encapsulated by graphitized mesoporous carbon

Nanoporous gold film based immunosensor for label-free detection of cancer biomarker

Effect of biolinker on the detection of prostate specific antigen in an interferometry

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