Semi-continuous feeding combined with traditional domestication improved anaerobic performance during treatment of cassava stillage

Lu, Fuzhi; Jiang, Qin; Feng, Qian; Zhou, Quanneng; Jiang, Chengjian; Shen, Peihong

doi:10.1016/j.biortech.2019.121807

Cited by 18 publications

(12 citation statements)

References 48 publications

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“…This suggested the co-existence of acetoclastic and hydrogenotrophic methanogenesis in both processes. The same phenomena were also reported by Lu et al [17] and Wang et al [14]. In the co-digestion process, Methanosarcinaceae was found as the second most abundant group after Methanosaetaceae.…”

Section: Composition Of Archaeal Communitysupporting

confidence: 86%

“…Fast DNA_ Spin Kit for Soil (Q-BIOgene, Carlsbad, USA) was used to extract genomic DNA from the samples according to the manufacturer's instructions. V3-V4 regions were ampli ed was targeted and primers 5′-ACTCCTACGGGAGGCAGCA-3′ and 5′-GGACTACHVGGGTWTCTAAT-3′ for bacteria Arch349F: 5′-GYGCASCAGKCGMGAAW-3′ and Arch806R: 5′-GGACTACVSGGGTATCTAAT-3′ for methanogens (Lu et al, 2019). PCR ampli cation was applied according to Shen et al (2014).…”

Section: Extraction Of Dna Pcr Ampli Cation and Sequencingmentioning

confidence: 99%

“…Methanogenesis, the last step, is considered to be the most critical part of the process with respect process failure due to variations in process conditions (temperature, pH, OLR and alkalinity) [14,15,16,17,18]. Therefore, depending on these conditions, stable process operation could only be possible when they co-exist in digesters.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of multiple anaerobic co-digestions and related microbial community of molasses with rice alcohol wastewater

Khan

Jiang

et al. 2020

Preprint

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Background Molasses is a highly dense and refined byproduct produced in the sugarcane industry, and it contains high amounts of degradable compounds. These compounds can potentially be converted into renewable products biologically. However, the involved biological process is negatively influenced by the high chemical oxygen demand (COD) of molasses and its high ion concentration, although this problem is commonly addressed by dilutions. Results The co-digestion of molasses with rice alcohol waste water (RAW) was compared with its mono-digestion at an increasing organic loading rate (OLR). Both processes were assessed by detecting the COD removal rate, the methane contents of biogas, and the structure and composition of microbial communities at different stages. Results showed that the co-digestion is stable up to a maximum OLR of 16 g COD L− 1d− 1. By contrast, after the acclimatization phase, the mono-digestion process was upset twice, which occurred at a maximum OLR of 9 and 10 g COD L− 1d− 1. The co-digestion procgess demonstrated consistency in terms of COD removal rates (86.36% ± 0.99–90.72% ± 0.63%) and methane contents (58.10% ± 1.12–64.47% ± 0.59%) compared with the mono-digestion process. Microbial community analysis showed that the relative abundance of bacterial and archaeal communities differs between the processes at different stages. However, in both processes, Propionibacteriaceae was the most abundant family in the bacterial communities, whereas Methanosaetaceae was abundant in the archaeal communities. Conclusion Rice alcohol wastewater could be a good co-substrate for anaerobic digestion of molasses. Integrate molasses into progressive biogas production at high OLR.

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Section: Composition Of Archaeal Communitysupporting

confidence: 86%

Section: Extraction Of Dna Pcr Ampli Cation and Sequencingmentioning

confidence: 99%

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Assessment of multiple anaerobic co-digestions and related microbial community of molasses with rice alcohol wastewater

Khan

Jiang

et al. 2020

Preprint

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“…For the extraction of genomic DNA, a Fast DNA Spin Kit for Soil (Q-BIOgene, Carlsbad, CA, USA) was used. V3-V4 regions were amplified and primers 5 -ACTCCTACGGGAGGCAGCA-3 and 5 -GGACTACHVGGGTWTCTAAT-3 were used for bacteria, while Arch349F: 5 -GYGCASCAGK CGMGAAW-3 and Arch806R: 5 -GGACTACVSGGGTATCTAAT-3 were used for methanogens [14]. PCR amplification was applied according to the protocol used by Shen et al [8].…”

Section: Extraction Of Dna Pcr Amplification and Sequencingmentioning

confidence: 99%

“…Methanogenesis is the last and most critical step of the process, mainly dependent on temperature, pH, OLR, and alkalinity. However, any fluctuation in these conditions may lead to process failure [13,14]. The anaerobic co-digestion process is a widely used technique providing a good platform for high biogas production.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Multiple Anaerobic Co-Digestions and Related Microbial Community of Molasses with Rice-Alcohol Wastewater

Khan

Jiang

et al. 2020

Energies

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Molasses is a highly dense and refined byproduct produced in the sugarcane industry, and it contains high amounts of degradable compounds. Through bioconversion, these compounds can be transformed into renewable products. However, the involved biological process is negatively influenced by the high chemical oxygen demand (COD) of molasses and ion concentration. The co-digestion of molasses with rice-alcohol wastewater (RAW) was compared with its mono-digestion at an increasing organic loading rate (OLR). Both processes were assessed by detecting the COD removal rate, the methane contents of biogas, and the structure and composition of microbial communities at different stages. Results showed that the co-digestion is stable up to a maximum OLR of 16 g COD L−1 d−1, whereas after the acclimatization phase, the mono-digestion process was disturbed two times, which occurred at a maximum OLR of 9 and 10 g COD L−1 d−1. The volatile fatty acids (VFAs) observed were 2059.66 mg/L and 1896.9 mg/L, which in mono-digestion causes the inhibition at maximum OLRs. In the co-digestion process, the concomitant COD removal rates and methane content recorded was 90.72 ± 0.63% 64.47% ± 0.59% correspondingly. While in the mono-digestion process, high COD removal rate and methane contents observed were 89.29 ± 0.094% and 61.37 ± 1.06% respectively. From the analysis of microbial communities, it has been observed that both the bacterial and archaeal communities respond differently at unlike stages. However, in both processes, Propionibacteriaceae was the most abundant family in the bacterial communities, whereas Methanosaetaceae was abundant in the archaeal communities. From the current study, it has been concluded that that rice-alcohol wastewater could be a good co-substrate for the anaerobic digestion of molasses in terms of COD removal rate and methane contents production, that could integrate molasses into progressive biogas production with high OLR.

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Energy Assessment and Inner Microbial Community Analysis of Internal Circulation (IC) Reactor for Bio-Hydrogen Production Using Brewery Wastewater

Feng

2022

Bioenerg. Res.

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Semi-continuous feeding combined with traditional domestication improved anaerobic performance during treatment of cassava stillage

Cited by 18 publications

References 48 publications

Assessment of multiple anaerobic co-digestions and related microbial community of molasses with rice alcohol wastewater

Assessment of multiple anaerobic co-digestions and related microbial community of molasses with rice alcohol wastewater

Assessment of Multiple Anaerobic Co-Digestions and Related Microbial Community of Molasses with Rice-Alcohol Wastewater

Energy Assessment and Inner Microbial Community Analysis of Internal Circulation (IC) Reactor for Bio-Hydrogen Production Using Brewery Wastewater

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