Selfing confirmation in sugarcane by using simple sequence repeat markers: an individual reciprocal recurrent selection scheme

Abstract

ABSTRACT. Superior inbred clones selected in S 1 families can integrate an individual reciprocal recurrent selection program in sugarcane by eliminating the genetic load of the population and exploring superior hybrid combinations. Molecular markers can be used for reliable identification of the true selfing-derived clones in these S 1 populations. The objective of this study was to confirm true self-fertilized individuals in sugarcane families using microsatellite markers aimed at the use of self-fertilized plants in an individual reciprocal recurrent selection strategy. Self-fertilized individuals Selfing confirmation and recurrent selection in sugarcane using SSR from five cultivars were genotyped with eight simple sequence repeat (SSR) markers. The markers generated 62 polymorphic markers, with an average of seven polymorphic alleles across the cultivars tested. Three loci revealed highly informative bands and were used to assess the level of selfing in five S 1 families. Selfing in these families ranged from 71.7 to 97.6%. The SSR loci provide a reliable and accurate method to identify S 1 progenies in sugarcane crosses and can be used as a tool to assist selection strategies in sugarcane breeding programs.

Microsatellite Genetic Markers of Saccharrum spp., and Erianthus sp. on Their Hybrids

“…In addition, Santos et al (2014) succeed identify 3-16 with the average 7.25 female specific alleles and 7-9 with the average 8.25 male specific alleles using three microsatellite marker at biparental crossing of commercial sugarcane varieties. Further, Costa et al (2014) could identify seven polymorphic alleles in average using eight microsatellite marker both in parent and selfs progenies.…”

“…Determination of male and female specific alleles is the first step that should be completed before the assessment of crossing fidelity (authentication) using DNA marker (Tew and Pan, 2010;Costa et al, 2014;Xavier et al, 2014;You et al, 2013;Fliho et al, 2010;Aitken et al, 2007;Pan, 2006). Xavier et al (2014) using 10 microsatellite primers and had succeed identifying 113.25 (73%) in average male specific alleles in progenies from polycross.…”

Fidelity of Sugarcane Crosses Assessed with SSR Markers

“…Sugarcane can also self-pollenate, particularly if excess pollen is in the vicinity. Several studies utilizing molecular markers have assessed parent identity, percent outcrossing, and the level of self-pollination from sugarcane crossing [5][6][7][8][9][10][11][12].…”

“…The simple sequence repeats (SSRs, also called microsatellites) method is one type of molecular marker and has the advantage of being highly polymorphic, highly abundant, with co-dominant inheritance, and analytically simple and readily transferable [13]. Microsatellites are often used to determine intentional hybridization (hybrids), selfing, and contamination in sugarcane seedlings [6][7][8][10][11][12]. Microsatellites or simple sequence repeat (SSR) markers have been successfully used to identify hybrids [14][15][16] and nucellar seedlings [17] in citrus and sugarcane hybrids [18].…”

“…Out of 154 progenies from two female parents, 22 were intentional hybrids, four were off-types, and 131 were selfs. Costa et al [7] intentionally self-pollinated five cultivars; however, the proportion of selfed seeds ranged from 71.7% to 83.3%. Santos et al [8] measured selfing, hybrid, and off-type rates among 76 progenies from four bi-parental crosses.…”