Self-sterility in the mutant ‘Zigui shatian’ pummelo (Citrus grandis Osbeck) is due to abnormal post-zygotic embryo development and not self-incompatibility

Chai, Lijun; Ge, Xianping; Biswas, Manosh Kumar; Xu, Qiang; Deng, Xiuxin

doi:10.1007/s11240-010-9793-6

Cited by 29 publications

(21 citation statements)

References 36 publications

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“…The sliced tissues were incubated in 8 N NaOH at 65 • C for 4-5 h for softening, macerated in 1% aniline blue solution at room temperature for 2 h continuously, and were finally smoothly squashed with a drop of glycerin (Chai et al, 2011). The slides were scanned and pictured by Leica DFC 450c camera (Leica microsystems, Wetzlar, Germany).…”

Section: Pollen Tube Growth After Pollinationmentioning

confidence: 99%

Induction of parthenogenetic haploid plants using gamma irradiated pollens in ‘Hirado Buntan’ pummelo (Citrus grandis [L.] Osbeck)

Wang

Jia

et al. 2016

Scientia Horticulturae

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Section: Pollen Tube Growth After Pollinationmentioning

confidence: 99%

Induction of parthenogenetic haploid plants using gamma irradiated pollens in ‘Hirado Buntan’ pummelo (Citrus grandis [L.] Osbeck)

Wang

Jia

et al. 2016

Scientia Horticulturae

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“…Pamelo potensial dikembangkan, karena karakteristiknya yang khas, yaitu berukuran besar, memiliki rasa segar, dan daya simpan yang lama sampai 4 bulan, dan beberapa kultivar hanya terdapat di Indonesia (Susanto, 2004) serta beberapa diantaranya tidak berbiji (Rahayu et al, 2012). Pamelo tidak berbiji juga dihasilkan negara lain terutama Thailand (Chai et al, 2011, Navareth et al, 2011 dan Jepang (Kim et al, 2011).…”

Section: Pendahuluanunclassified

Perbaikan Pembungaan Pamelo melalui Aplikasi Strangulasi dan Zat Pemecah Dormansi

Susanto¹,

Melati²,

Sugeru³

2017

J Hort Indonesia

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ABSTRACT Pummelo productivity is still low, therefore efforts should be made to increase its flowering and production. The objective of study was to determine the effectiveness of strangulation combined with the application of breaking dormancy substances to improve flowering of pummelo. The experiment was conducted in January to November 2015, at the Cikabayan Experimental Research Station, IPB. The carbohydrate and nitrogen analysis was done in laboratory of Postharvest Agriculture Research Institute, Bogor. Three-year-old pummelo grown in field was subjected for this research. Experiment used a completely randomized design (CRD) with two factors. The first factor was treatment to stimulate flowering, consisted of 3 levels i.e. single strangulation, double strangulation and control, and the second factor was the use of dormancy breaking substances, consisted of 3 types i.e. KNO3, Ethepon and BAP. Strangulation was performed by pressing the wire with a diameter of 2.0 mm into stem as deep as the diameter of the wire. Strangulation was done simultaneously and then released after 3 months. Dormancy breaking substance was applied immediately after releasing the wire for strangulation with the concentration of 200 ppm KNO3, 100 ppm Ethepon or 100 ppm BA. Treatments were replicated 4 times. The results showed that single and double strangulation treatments for 3 months were an effective way to induce flowering of young pummelo trees. Double strangulation produced more flowers as compared to single strangulation. Increased carbohydrate content and C/N ratio in leaves were observed on flower induced trees. Strangulation treatment for 3 months can increase flowering of young pummelo trees. Application of dormancy breaking substances did not have any effect on flowering induction in pummelo.Keywords: carbohydrate content, dormancy breaking substance, flower induction, pummelo, strangulationABSTRAK Produktivitas pamelo masih rendah sehingga perlu upaya peningkatan pembungaan dan produksinya. Penelitian ini bertujuan untuk mengetahui efektivitas strangulasi yang dikombinasikan dengan aplikasi zat pemecah dormansi dalam meningkatkan pembungaan jeruk pamelo. Penelitian dilaksanakan pada bulan Januari sampai Nopember 2015, bertempat di Kebun Percobaan IPB Cikabayan. Analisis karbohidrat dan nitrogen dilakukan di laboratorium BB Pasca Panen, Bogor. Percobaan menggunakan tanaman jeruk pamelo “Nambangan’ berumur 3 tahun dengan Rancangan Acak Lengkap (RAL) dua faktor. Sebagai faktor pertama yaitu perlakuan untuk menstimulasi pembungaan, terdiri atas 3 taraf perlakuan yaitu perlakuan strangulasi tunggal, strangulasi ganda dan kontrol, dan sebagai faktor kedua adalah penggunaan zat pemecah dormansi terdiri atas 3 jenis yaitu KNO3, Ethepon dan BAP. Masing-masing perlakuan diulang 4 kali sehingga terdapat 36 unit percobaan. Strangulasi dilaksanakan dengan melilitkan kawat dengan diameter 2.0 mm pada batang dengan menekan kawat ke batang sedalam diameter kawat tersebut. Strangulasi dilakukan serentak pada batang, strangulasi dilepas setelah 3 bulan kemudian. Zat pemecah dormansi diaplikasikan segera setelah pelepasan kawat strangulasi dengan konsentrasi masing-masing 200 ppm untuk KNO3, 100 ppm untuk Ethepon atau 100 ppm untuk BAP. Hasil penelitian menunjukkan perlakuan strangulasi selama 3 bulan merupakan cara efektif menginduksi pembungaan tanaman jeruk pamelo. Perlakuan strangulasi ganda menghasilkan bunga lebih banyak dibandingkan dengan strangulasi tunggal. Tanaman yang telah terinduksi menunjukkan tingginya kandungan karbohidrat pada tajuk tanaman sehingga meningkatkan rasio C/N. Perlakuan zat pemecah dormansi tidak memberikan pengaruh yang nyata terhadap pembungaan jeruk pamelo. Kata kunci: kandungan karbohidrat, pamelo, pembungaan, strangulasi, zat pemecah dormansi

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“…Auto-incompatibility derives from the failure in fecundation processes by way of pollen from the same plant or with pollen from plants with the identical genetic constitution of the receptor plant (Chai et al, 2011;Distefano et al, 2009;Miao et al, 2011;Ye et al, 2009). The grove at which URS Campestre plants are located has many other citrus cultivars (cv.…”

Section: Fruit Qualitymentioning

confidence: 99%

‘URS Campestre’ seedless orange: a new mutant with female sterility

Bender

Santos

Guerra

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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Seedlessness in fruit is a trait that is much sought after by juice making industries.Close to the city of São Sebastião do Caí, in the state of Rio Grande do Sul (RS), Brazil, a new mutant orange originating from natural mutation was identified and selected as a seedless material. To determine the mechanisms involved in the absence of seeds, the reproductive structures of this new mutant by comparison with a Valencia sweet orange as control, a cultivar with a profusion of seeds, was analyzed in terms of meiotic behavior, meiotic index, pollen viability, in vitro germination, and ovule features to determine the grounds for seed absence.Other morphological analyzes allowed for visualizing the structures of normal appearance and size in both cultivars. Meiotic analysis identified chromosome normal pairing with a predominance of bivalents at diakinesis and metaphase 1. URS Campestre flowers at different developmental stages had anthers and ovaries whose dimensions are typical while pollen grain analysis pointed to a standard developmental pattern, normal meiosis, high viability (84 %) and elevated in vitro pollen tube germination rates (63 %). The cv. Valencia and URS Campestre ovules had a similar shape and morphology, sharing an anatropous orientation, and two integuments. In the internal ovule analyses of Valencia sweet oranges, normal embryo sac cells were identified: presence of one egg cell and two synergids, three antipodes and a bigger and central cell containing two polar nuclei. However, the analysis of ovules from URS Campestre reveals an apparent senescence or non-formation of an embryo sac, where only a few highly stained and collapsed cells could be identified. These results led to the conclusion that female sterility in URS Campestre, with a total absence of a female gametophyte, is the limiting factor for fertilization and seed production.

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Self-sterility in the mutant ‘Zigui shatian’ pummelo (Citrus grandis Osbeck) is due to abnormal post-zygotic embryo development and not self-incompatibility

Abstract: Shatian' pummelo (Citrus grandis Osbeck)

Cited by 29 publications

References 36 publications

Induction of parthenogenetic haploid plants using gamma irradiated pollens in ‘Hirado Buntan’ pummelo (Citrus grandis [L.] Osbeck)

Induction of parthenogenetic haploid plants using gamma irradiated pollens in ‘Hirado Buntan’ pummelo (Citrus grandis [L.] Osbeck)

Perbaikan Pembungaan Pamelo melalui Aplikasi Strangulasi dan Zat Pemecah Dormansi

‘URS Campestre’ seedless orange: a new mutant with female sterility

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