Induction of parthenogenetic haploid plants using gamma irradiated pollens in ‘Hirado Buntan’ pummelo (Citrus grandis [L.] Osbeck)

Wang, Shuming; Li, Hong; Jia, Hui-Hui; Xie, Kai‐Dong; Wu, Xiaomeng; Chen, Chunli; Guo, Wen‐Wu

doi:10.1016/j.scienta.2016.05.028

Cited by 15 publications

(7 citation statements)

References 46 publications

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“…In citrus, this technique has proven to be very useful to obtain haploid plants with high value for breeding. For example, Wang et al [49] were able to induce haploid plants in Citrus grandis L. Osbeck by irradiating pollen with γ-rays with doses lower than 500 Gys and in vitro culture of immature embryos. Likewise, Jedidi et al [52], by irradiating pollen at 250 Gys with γ-rays, obtained seven seedlings that were used to generate homozygous lines in Citrus reticulata Blanco.…”

Section: Irradiated Pollenmentioning

confidence: 99%

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Marín-Montes

Rodríguez-Pérez

Robledo-Paz

et al. 2022

Plants

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The generation of new hybrid varieties of tomato (Solanum lycopersicum L.) is the most widely used breeding method for this species and requires at least seven self-fertilization cycles to generate stable parent lines. The development of doubled haploids aims at obtaining completely homozygous lines in a single generation, although, to date, routine commercial application has not been possible in this species. In contrast, obtaining doubled haploid lines via gynogenesis has been successfully implemented in recalcitrant crops such as melon, cucumber, pumpkin, loquat and walnut. This review provides an overview of the requirements and advantages of gynogenesis as an inducer of haploidy in different agricultural crops, with the purpose of assessing the potential for its application in tomato breeding. Successful cases of gynogenesis variants involving in vitro culture of unfertilized ovules, use of 60Co-irradiated pollen, in vivo haploid inducers and wide hybridization are presented, suggesting that these methodologies could be implemented in tomato breeding programs to obtain doubled haploids.

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Section: Irradiated Pollenmentioning

confidence: 99%

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Marín-Montes

Rodríguez-Pérez

Robledo-Paz

et al. 2022

Plants

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“…Kurtar (2009) reported viability rate of non-irradiated pollens in squash was 90%, this rate decreased as the irradiation dose increased and the pollen viability rate decreased to 17% in the 300 Gy dose applications. On the other hand, no significant differences were observed in different irradiation doses on pollen viability in 'Majia' pomelo by Yang et al (2020) and trifoliate orange by Wang et al (2016). These results suggest that increase or decrease in pollen viability is primarily due to the different reactions of plant species, cultivars or genotypes according to the amount and duration of the applied irradiation dose.…”

Section: Discussionmentioning

confidence: 66%

Assessment of The Effect of Gamma Ray Treatments on Pollen Behavior in Cyclamen

Tütüncü

Mendi

2022

Uluslararası Tarım Ve Yaban Hayatı Bilimleri Dergisi

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The aim of the study is to evaluate the effects of gamma irradiation on pollen grains of Cyclamen persicum L. which is a valuable ornamental pot plant. Pollens were irradiated at different doses (0, 50, 100, 150, 200, 300, 450 Gy) of gamma-ray using Co-60 source and pollen viability and in vitro pollen germination test were carried out and tube length of in vitro germinated pollens was measured. Additionally, in vivo pollen development was visualized by performing an aniline blue fluorescence method. Average pollen viability was ranged from 82.02% to 87.03%. The highest pollen germination rate (66.13%) was observed in the control group at 24 h after irradition, while the lowest rate (28.51%) was determined in 450 Gy treatments at 72 h after irradition. The shortest pollen tube length (48.02 µm) was observed at 72 h old non-irradiated pollen grains, while the most extended pollen tube length (79.37 µm) was determined in pollen grains irradiated with 300 Gy at 24 HAI. When the irradiation dose increases from 150 Gy to 450 Gy, inhibition on pollen germination and pollen tube elongation within the style were observed.

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“…Histograms form each regenerated plantlet were generated automatically from an analysis of at least 3,000 nuclei. Chromosome counting analysis was performed with root-tips from randomly selected polyploid progeny to determine their ploidy level as described by Wang et al [23] . The chromosomes were counterstained with 4, 6-diamidino-2-phenylindole, mounted in Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA), and examined with a Zeiss Imager.M2 fluorescence microscope (Zeiss, Oberkochen, Germany).…”

Section: Ploidy Analysismentioning

confidence: 99%

Unreduced Megagametophyte Formation via Second Division Restitution Contributes to Tetraploid Production in Interploidy Crosses With 'Orah' Mandarin (Citrusreticulata)

2021

Front. Agr. Sci. Eng.

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HIGHLIGHTS • In addition to triploid progeny, tetraploid hybrids derived from the fertilization of 2n megagametophytes are frequently regenerated from 2x × 4x crosses that utilize 'Orah' mandarin as the female parent. • Data here indicate that 'Orah' mandarin is a cultivar that readily produces 2n megagametophytes. • Second division restitution is the mechanism underlying 2n megagametophyte formation in 'Orah' mandarin.

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Induction of parthenogenetic haploid plants using gamma irradiated pollens in ‘Hirado Buntan’ pummelo (Citrus grandis [L.] Osbeck)

Cited by 15 publications

References 46 publications

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Assessment of The Effect of Gamma Ray Treatments on Pollen Behavior in Cyclamen

Unreduced Megagametophyte Formation via Second Division Restitution Contributes to Tetraploid Production in Interploidy Crosses With 'Orah' Mandarin (Citrusreticulata)

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