Self-standardization of quality of bacterial cellulose produced by Medusomyces gisevii in nutrient media derived from Miscanthus biomass

Скиба, Е. А.; Гладышева, Е. К.; Golubev, Dmitri S.; Будаева, В. В.; Алешина, Л. А.; Сакович, Г. В.

doi:10.1016/j.carbpol.2020.117178

Cited by 23 publications

(21 citation statements)

References 44 publications

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“…Works on enhancing the BNC yield include the following directions: a search for and genetic construction of new high-productivity microbial producers [1,5,21,22]; design of new technological approaches to cultivation [5,20,21,23]; and a quest for alternative and cheap raw sources [5,[24][25][26][27][28][29]. Sharma [5] stresses that the high capital investment together with high operating costs is the major constraint towards commercialization of BNC at a reasonable cost.…”

Section: Introductionmentioning

confidence: 99%

Scale-Up of Biosynthesis Process of Bacterial Nanocellulose

et al. 2021

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Bacterial nanocellulose (BNC) is a unique product of microbiological synthesis, having a lot of applications among which the most important is biomedicine. Objective complexities in scaling up the biosynthesis of BNC are associated with the nature of microbial producers for which BNC is not the target metabolite, therefore biosynthesis lasts long, with the BNC yield being small. Thus, the BNC scale-up problem has not yet been overcome. Here we performed biosynthesis of three scaled sheets of BNC (each having a surface area of 29,400 cm2, a container volume of 441 L, and a nutrient medium volume of 260 L and characterized them. The static biosynthesis of BNC in a semisynthetic nutrient medium was scaled up using the Medusomyces gisevii Sa-12 symbiotic culture. The experiment was run in duplicate. The BNC pellicle was removed once from the nutrient medium in the first experiment and twice in the second experiment, in which case the inoculum and glucose were not additionally added to the medium. The resultant BNC sheets were characterized by scanning electron microscopy, capillary viscosimetry, infrared spectroscopy, thermomechanical and thermogravimetric analyses. When the nutrient medium was scaled up from 0.1 to 260 L, the elastic modulus of BNC samples increased tenfold and the degree of polymerization 2.5-fold. Besides, we demonstrated that scaled BNC sheets could be removed at least twice from one volume of the nutrient medium, with the yield and quality of BNC remaining the same. Consequently, the world’s largest BNC sheets 210 cm long and 140 cm wide, having a surface area of 29,400 cm2 each (weighing 16.24 to 17.04 kg), have been obtained in which an adult with burns or vast wounds can easily be wrapped. The resultant sheets exhibit a typical architecture of cellulosic fibers that form a spatial 3D structure which refers to individual and extremely important characteristics of BNC. Here we thus demonstrated the scale-up of biosynthesis of BNC with improved properties, and this result was achieved by using the symbiotic culture.

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Section: Introductionmentioning

confidence: 99%

Scale-Up of Biosynthesis Process of Bacterial Nanocellulose

et al. 2021

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“…FTIR can prove that the test substance is actually cellulose. For the microbial producer we used, that was demonstrated in our previous studies many times [ 19 , 32 , 39 , 50 ]. Besides, FTIR enables the measurements of the crystallinity index and the ratio of allomorph I α to I β in BC, more specifically these parameters allow the individual structure of BC to be identified, that is, how one type of BC differentiates from another BC type and what applications it may find.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous paper [ 19 ], it was demonstrated that the variation in the nutrient medium composition and the inhibitors of BC biosynthesis contained therein did not alter the index of crystallinity and the I α to I β allomorph ratio in the BC test samples. The values were very high: the index of crystallinity was 86–94% and the allomorph I α content was 99–100%.…”

Section: Resultsmentioning

confidence: 99%

“…Such a broad demand for BC is a powerful driving force for further research. Studies on synthesis methods of BC can be divided into three domains: (1) finding and genetic construction of new, highly productive microbial producers [ 1 , 9 , 16 ]; (2) development of new technological approaches to cultivation [ 4 ]; and (3) search for cheap alternative feedstocks [ 17 , 18 , 19 , 20 ]. The synthesis methods of BC determine BC properties which, in turn, determine its applications [ 2 , 10 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles

et al. 2021

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Extended cultivation with multiple removal of BC pellicles is proposed herein as a new biosynthetic process for bacterial cellulose (BC). This method enhances the BC surface area by 5–11 times per unit volume of the growth medium, improving the economic efficiency of biosynthesis. The resultant BC gel-films were thin, transparent, and congruent. The degree of polymerization (DP) and elastic modulus (EM) depended on the number of BC pellicle removals, vessel shape, and volume. The quality of BC from removals II–III to VII was better than from removal I. The process scale-up of 1:40 by volume increased DP by 1.5 times and EM by 5 times. A fact was established that the symbiotic Medusomyces gisevii Sa-12 was adaptable to exhausted growth medium: the medium was able to biosynthesize BC for 60 days, while glucose ran low at 24 days. On extended cultivation, DP and EM were found to decline by 39–64% and 57–65%, respectively. The BC gel-films obtained upon removals I–VI were successfully trialed in experimental tension-free hernioplasty.

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“…volatile acids, lignohumic substances, formaldehyde, furfural, oxymethylfurfural, etc. These compounds appear as a result of the combined action of chemical agents and such physical factors as temperature and pressure [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Miscanthus sinensis Cellulose by Bioconversion

Kriger

Babich

Dolganyuk

et al. 2021

Food Processing: Techniques and Technology

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Introduction. Cellulose-containing parts of herbs are an excellent source of alternative energy and can be used to produce biological ethanol. The present research aims at improving this fundamental and promising area of biotechnology. It introduces a new consortium of microorganisms that can saccharify while fermenting the substrate. Study objects and methods. The research featured technical cellulose obtained from Miscanthus sinensis using hydrotropic delignification and oxidation with pertrifluoroacetic acid. The ethanol content in the culture liquid was determined using an Agilent 7890B gas chromatograph with a flame ionization detector. The biocompatibility of the strains was studied by growing a direct co-culture in a dense nutrient medium. Results and discussion. The research objective was to create a new microbial consortium for the single-step production of bioethanol from Miscanthus sinensis cellulose. A set of biocompatibility experiments and cultivation conditions made it possible to select the optimal producers. The two developed microbial consortia required optimal compositions of culture media, which were determined by varying the ratio of components and measuring the yield of ethanol in the resulting culture liquid. Conclusion. The best consortium for Miscanthus sinensis cellulose consisted of Pichia stipites Y7124, Candida shehatae NCL3501, Kluyveromyces marxianus Y-4290, and Zymomonas mobilis 113 at a ratio of 1:1:1:1. The optimal parameters of bioethanol production included: temperature = 35 ± 1°C, pH = 5.2, time = 16 ± 1 h. The most efficient culture medium had the following composition (g/l): glucose – 5.0; peptone – 5.0; yeast extract – 0.4; K2HPO4 – 1.5; (NH)2 HPO4 – 1.5; MgSO4 – 0.5.

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Self-standardization of quality of bacterial cellulose produced by Medusomyces gisevii in nutrient media derived from Miscanthus biomass

Cited by 23 publications

References 44 publications

Scale-Up of Biosynthesis Process of Bacterial Nanocellulose

Scale-Up of Biosynthesis Process of Bacterial Nanocellulose

Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles

Bioethanol Production from Miscanthus sinensis Cellulose by Bioconversion

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