Self-assembly hollow nanosphere for enzyme encapsulation

Ha, Wei; Meng, Xianwei; Li, Quan; Fan, Ming‐Yu; Peng, Shu‐Lin; Ding, Li‐Sheng; Tian, Xuan; Zhang, Sheng; Li, Bang‐Jing

doi:10.1039/b925747k

Cited by 47 publications

(45 citation statements)

References 20 publications

(22 reference statements)

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“…The encapsulated L-asparaginase showed significantly higher stability and maintained most of the activity. 21 In this paper, we expand the study on evaluating the permeability properties of Alg-g-PEG/a-CD hollow nanospheres. Vitamin B 2 , vitamin B 12 and four globular shape proteins with different molar masses were used as permeability probes.…”

Section: -19mentioning

confidence: 99%

“…21,23 In this case, for synthesis of Alg-g-PEG, a 2% (w/w) sodium alginate solution was prepared in a buffer solution of 0.1 M MES and 0.5 M NaCl, and the pH was adjusted to 6. The molar ratio of EDC : NHS : COO À was 1 : 0.5 : 1, a series of different moles of NHS and EDC were added to a 2% (w/w) alginate solution to activate the carboxy groups on the polymer backbone.…”

Section: Synthesis Of the Alg-g-pegmentioning

confidence: 99%

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Encapsulation studies and selective membrane permeability properties of self-assembly hollow nanospheres

Meng

et al. 2011

Soft Matter

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This paper presents a kind of self-assembled hollow nanosphere for enzyme encapsulation. The enzyme molecules were encapsulated in hollow spheres directly in aqueous solution through the self-assembly of rod-coil Alg-g-PEG and a-CDs complexes. These Alg-g-PEG/a-CD hollow spheres showed semipermeability which could prevent big enzyme molecules from leaving while allowing small substrates and products to pass through to maintain the enzyme activity. The permeability properties of Alg-g-PEG/a-CD hollow nanospheres were investigated by encapsulating different probes. The results showed that the molar mass cutoff (MMCO) of these hollow nanospheres was between 20 and 40 kDa. The encapsulation capability of Alg-g-PEG/a-CD hollow nanospheres was also investigated, the results indicated a maximal values for L-asparaginase encapsulation. Furthermore, the encapsulation behavior for L-asparaginase showed PEG graft density (GD) dependence.

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Section: -19mentioning

confidence: 99%

Section: Synthesis Of the Alg-g-pegmentioning

confidence: 99%

Encapsulation studies and selective membrane permeability properties of self-assembly hollow nanospheres

Meng

et al. 2011

Soft Matter

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“…The encapsulated enzymes exhibited improved stability. 55,67,68 The CD-based hollow spheres can also be used to build a biomimetic energy converter for ATP biosynthesis. 69 As shown in Fig.…”

Section: View Article Onlinementioning

confidence: 99%

Nanoassemblies driven by cyclodextrin-based inclusion complexation

Kang

Guo

et al. 2014

Chem. Commun.

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Nanoscaled supramolecular systems have attracted significant attention because of their promising applications in many fields. This review focuses on recent advances in the construction of nanoassemblies driven by cyclodextrin (CD)-based inclusion complexation and their application in biomedical and biomimetic fields. As a result of the reversibility of the CD-based host-guest interactions, CD-based driving forces provide the opportunity to generate complex and sophisticated nanoassemblies with tunable properties.

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“…As shown in Figure 1a, the particles had round outline shape with diameter of about 500 nm and the cores of particles were a little brighter than the outlines of the particles, which was a typical TEM image of hollow spheres as reported for different kinds of hollow particles. [13][14][15][16][17][18]22 After addition of GOD to the solution during the process of self-assembly of CS-g-PEGM and α-CD, the enzymes were entrapped in the CS-g-PEGM/α-CD hollow spheres. The isoelectric point of GOD and CS was around 4.2 and 6.3, respectively, 25 therefore the pH of mixture was adjusted to 6.8 to make sure that the GODs could not absorb on CS-g-PEGM through electrostatic interaction.…”

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Cyclodextrin-Based Microcapsules as Bioreactors for ATP Biosynthesis

Jian-hu

Wang

et al. 2013

Biomacromolecules

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A biomimetic energy converter was fabricated via the assembly of CF 0 F 1 -ATPase on lipid-coated hollow nanocapsules composed of α-cyclodextrins/ chitosan-graf t-poly(ethylene glycol) methacrylate. Upon entrapped GOD into these capsules, the addition of glucose could trigger proton-motive force and then drive the rotation of ATPase to synthesize ATP.L iving organisms make a series of metabolisms every moment. An organism that wants to maintain activities needs a continuous supply of energy: adenosine triphosphate (ATP). As the energy currency, ATP induces large conformational changes in molecules such as myosin, kinesin, and chaperonin. With inspiration from biology, biological and biomimetic devices that supply ATP as energy, such as molecular motors and artificial cell bioreactors, have been developed. 1−4 However, in motility and functionality assays reported on these devices, the issue of ATP regulation is usually sidestepped by supplying ATP externally into the buffer solution. Complications can occur as high concentrations of ATP, and the devices stop operation when all the ATP runs out. For the long-term operation of more complex molecular devices, continual supply of the right amount of ATP is required.The enzyme primarily responsible for the production of ATP is the F 1 F 0 -type ATPase, which synthesizes ATP from adenosine diphosphate (ADP) and inorganic phosphate (Pi) by utilizing proton gradients. 5 Several groups have been working on the reconstitution of ATPase in liposomes as biomimetic systems to carry out ATP biosynthesis. 6−8 Although they have excellent assay characteristics, the future applicability of liposomes is intrinsically limited due to chemical and mechanical weaknesses. In order to get more stable and robust biomimetic membranes, polymersome and lipid bilayercoated microcapsules have been developed replacing traditional liposomes very recently. 9−12 For instance, Choi et al. demonstrated that synthesized amphiphilic polymersomes can replace liposomes for ATP synthesis. However, the synthesis needs strict control, and the assembled vesicles were not sufficiently stable. Li et al. introduced microcapsules fabricated by the layer-by-layer (LBL) assembly technique into the biomimetic system design and fabricated a series of biomimetic energy converters via the assembly of ATPase on lipid-coated hollow microcapsules. However, this strategy requires a procedure to remove the core in order to create a hollow interior and needs multiple steps to produce the wall of the microcapsules.In our previous study, we reported that polymeric hollow spheres with semipermeability could be obtained directly by self-assembly of rod−coil copolymers/cyclodextrin (CD) complexes in analogy to the cell membrane. 13−18 In the present work, we utilized this self-assembly approach to design a new type of biomimetic capsules for ATP synthesis. This capsule comprised a self-assembled hollow sphere as the inner support and its absorbed lipid as the out layer. Furthermore, glucose oxidases (GODs) were ent...

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Self-assembly hollow nanosphere for enzyme encapsulation

Cited by 47 publications

References 20 publications

Encapsulation studies and selective membrane permeability properties of self-assembly hollow nanospheres

Encapsulation studies and selective membrane permeability properties of self-assembly hollow nanospheres

Nanoassemblies driven by cyclodextrin-based inclusion complexation

Cyclodextrin-Based Microcapsules as Bioreactors for ATP Biosynthesis

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