Encapsulation studies and selective membrane permeability properties of self-assembly hollow nanospheres

Abstract

This paper presents a kind of self-assembled hollow nanosphere for enzyme encapsulation. The enzyme molecules were encapsulated in hollow spheres directly in aqueous solution through the self-assembly of rod-coil Alg-g-PEG and a-CDs complexes. These Alg-g-PEG/a-CD hollow spheres showed semipermeability which could prevent big enzyme molecules from leaving while allowing small substrates and products to pass through to maintain the enzyme activity. The permeability properties of Alg-g-PEG/a-CD hollow nanospheres were investigated by encapsulating different probes. The results showed that the molar mass cutoff (MMCO) of these hollow nanospheres was between 20 and 40 kDa. The encapsulation capability of Alg-g-PEG/a-CD hollow nanospheres was also investigated, the results indicated a maximal values for L-asparaginase encapsulation. Furthermore, the encapsulation behavior for L-asparaginase showed PEG graft density (GD) dependence.

Recent advances in the solution self‐assembly of amphiphilic “rod‐coil” copolymers

“…New rod‐coil graft copolymers with polypeptide, polysaccharide, or alginate blocks have been recently synthesized for construction of self‐assembled structures in solution, which are particularly interesting for biological, medical, and biotechnological applications . Park et al designed a kind of facial peptides containing tryptophan units, tyrosine derivatives with tri(ethylene glycol) monomethyl ether, and terminal lysine units [Fig.…”

“…In addition, these CKGM‐ g ‐PEG/α‐CD nanospheres showed in vitro biocompatibility when exposed to L929 cells during the test using MTT viability assay, suggesting that the self‐assembly of CKGM‐ g ‐PEG and α‐CD to form stable nanoparticles can be an effective strategy for enzyme encapsulation with potential biomedical applications. In another study, hollow spheres were also prepared by self‐assembly of rod‐coil Alginate‐ g ‐PEG/α‐CD complexes in water, in which the rod blocks were formed by inclusion between α‐CDs and grafted PEG chains while the coil segments were protonated alginate backbones . The rod blocks formed the wall of the hollow spheres and the protonated alginate coils constructed the coronae.…”

Self‐Assembly Pluronic and β‐Cyclodextrin to Hollow Nanospheres for Enhanced Gene Delivery

“…Furthermore, it is found that the supramolecular hollow particles could encapsulate enzyme molecules, effectively prevent the enzyme from leaking, and maintain enzyme activity. [20,21] In this study, the work is extended to encapsulate PEI10K/DNA complexes in supramolecular hollow spherical aggregates for gene delivery. Poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) copolymer (also known as pluronic) is selected as building blocks for the preparation of hollow particles since it has been used extensively in a variety of pharmaceutical formulations including drugs and gene delivery.…”

Nanoassemblies driven by cyclodextrin-based inclusion complexation

“…The encapsulated enzymes exhibited improved stability. 55,67,68 The CD-based hollow spheres can also be used to build a biomimetic energy converter for ATP biosynthesis. 69 As shown in Fig.…”