Self‐Assembly Pluronic and β‐Cyclodextrin to Hollow Nanospheres for Enhanced Gene Delivery

Fan, Ming‐Yu; Zhang, Xi; Qin, Jie; Li, Bang‐Jing; Sun, Xun; Zhang, Sheng

doi:10.1002/marc.201100272

Cited by 21 publications

(9 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One kind of nanoassembly was hollow nanospheres based on inclusion complexation between b-CD and PEG-PPG-PEG (F127), which could encapsulate PEI/DNA in the hollow core. 63,64 The resulting complexes displayed similar or more efficient gene transfection in comparison with PEI/DNA, but much less cytotoxicity. Another kind of nanoassembly was rod-like nanoparticles prepared by self-assembly of a-CD and PEG-PEI.…”

Section: Gene Deliverymentioning

confidence: 99%

Nanoassemblies driven by cyclodextrin-based inclusion complexation

Kang

Guo

et al. 2014

Chem. Commun.

Self Cite

View full text Add to dashboard Cite

Nanoscaled supramolecular systems have attracted significant attention because of their promising applications in many fields. This review focuses on recent advances in the construction of nanoassemblies driven by cyclodextrin (CD)-based inclusion complexation and their application in biomedical and biomimetic fields. As a result of the reversibility of the CD-based host-guest interactions, CD-based driving forces provide the opportunity to generate complex and sophisticated nanoassemblies with tunable properties.

show abstract

Section: Gene Deliverymentioning

confidence: 99%

Nanoassemblies driven by cyclodextrin-based inclusion complexation

Kang

Guo

et al. 2014

Chem. Commun.

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has been observed that the incorporation of drugs into the hydrophobic PO core of these micelles increases the solubility, metabolic stability and circulation time during its journey towards the target . However, very less studies are available showing the use of pluronic for gene delivery . It was observed that pluronic block copolymers increase the expression of pDNA in skeletal muscle in mice .…”

Section: Introductionmentioning

confidence: 99%

EDTA‐Capped Iron Oxide Core‐Corona System as Vehicle for Gene Delivery to Transform E.coli: Mimicking the Lipid Bilayer Environment

et al. 2019

View full text Add to dashboard Cite

EDTA‐capped iron oxide nanoparticles (Fe3O4/edta NPs) were synthesized by simple wet chemical method and encapsulated with amphiphilic triblock copolymer Pluronic®F127 (Fe3O4/edta/P). The resulting ‘core‐corona’ system becomes capable of condensing plasmid DNA (pDNA) and act as vehicle for cell transformation. As a proof‐of‐concept, E.coli_DH5α bacterial strain was selected. The plasmids like pBSKS and pUC18 having antibiotic resistance marker gene were eluted from the bacterial strain, purified and loaded on the synthesized nano‐assembly. Green fluorescence protein tag was attached to pTX (pTXgfp) to confirm successful transformation. The gene transformation efficiency (TE) of the developed Fe3O4/edta/P vehicle was compared with traditional CaCl2 mediated transformation as a positive control and naked pTXgfp was used as a negative control. PEGlation of Fe3O4/edta was also carried out and the TE of the resulting system was compared with as‐synthesized Fe3O4/edta/P. This study demonstrates that Pluronic® F127 polymeric surfactant can be used to form stealth surface of the nanocarrier to solve Polyethylene Glycol (PEG) dilemma. The probable mechanism to explain the bacterial cell transformation is discussed.

show abstract

“…[27][28][29][30][31][32] Among the various supramolecular assemblies, CD-based (Pseudo) polyrotaxanes have been extensively studies for their uses as carriers in drug delivery, such as thermo-/pH-sensitive hydrogels, [33][34][35] vesicles and micelles, as carriers for the controlled drug release 36 and gene carriers. [37][38][39] However, (Pseudo) polyrotaxanes, self-assembled from cyclodextrins (CDs) and polyesters, were apt to form crystallized precipitate in most of the solvent, especially in water, which profoundly limited their development in biomedical applications. In this article, amphiphilic block copolymers with novel architecture, a pH-sensitive amphiphilic dendritic polyrotaxane polymer-drug conjugate (PR-g-DOX), were successfully developed through a highly efficient approach.…”

Section: Introductionmentioning

confidence: 99%

pH-responsive dendritic polyrotaxane drug-polymer conjugates forming nanoparticles as efficient drug delivery system for cancer therapy

Kang

Zhang

et al. 2015

Polym. Chem.

Self Cite

View full text Add to dashboard Cite

a Self-assembly of stimuli-responsive polymeric nanoparticles have attracted great attention in recent years due to their prospective biological applications. This paper developed a novel pH-sensitive amphiphilic dendritic polyrotaxane drug-polymer conjugate by covalently linking doxorubicin (DOX) and dendritic polyrotaxane via a pH-responsible hydrazone bond with 1.84 wt% (weight percent) of DOX. The drugpolymer conjugate was amphiphilic and could be self-assembled to micelles (PR-g-DOX micelles) in an aqueous solution. The globular morphology and compact micelles with a diameter of around 110 nm were observed by SEM and TEM. Moreover, the micelles showed a significantly faster DOX release at mildly acidic pH of 6.0 and 5.0 and almost no burst release at the physiological pH of 7.4. Notably, it was confirmed that this micelle could efficiently deliver DOX to the nuclei of the tumor cells, which led to a much greater cytotoxic effects in the A549 cancer cells than the parent DOX. In vivo results revealed better drug tolerability of PR-g-DOX micelles and a higher in vivo efficacy without any increase in the toxicity of the PR-g-DOX micelles. Furthermore, the maximum tolerated dose (MTD) results showed that PRg-DOX micelles showed an excellent safety profile with a two-fold higher MTD (10 mg kg −1 DOX) than that of free DOX (5 mg kg −1 DOX). We are convinced that the PR-g-DOX micelle has tremendous potentials for targeted cancer therapy.

show abstract

Self‐Assembly Pluronic and β‐Cyclodextrin to Hollow Nanospheres for Enhanced Gene Delivery

Cited by 21 publications

References 31 publications

Nanoassemblies driven by cyclodextrin-based inclusion complexation

Nanoassemblies driven by cyclodextrin-based inclusion complexation

EDTA‐Capped Iron Oxide Core‐Corona System as Vehicle for Gene Delivery to Transform E.coli: Mimicking the Lipid Bilayer Environment

pH-responsive dendritic polyrotaxane drug-polymer conjugates forming nanoparticles as efficient drug delivery system for cancer therapy

Contact Info

Product

Resources

About