Selenite-stress selected mutant strains of probiotic bacteria for Se source production

Pusztahelyi, Tünde; Kovács, Szilvia; Pócsi, István; Prokisch, József

doi:10.1016/j.jtemb.2014.11.003

Cited by 34 publications

(23 citation statements)

References 32 publications

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“…Additionally, we found that the genus Bifidobacterium exhibited significant positive correlation with the dietary selenium (Se). The interaction between the Se and the genus Bifidobacterium had been reported in previous study, and the supplementary Se in MRS ager was able to promote the growth of the Bifidobacterium 25 .…”

Section: Discussionsupporting

confidence: 71%

Unique Features of Ethnic Mongolian Gut Microbiome revealed by metagenomic analysis

Liu

Zhang

Wu³

et al. 2016

Sci Rep

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The human gut microbiota varies considerably among world populations due to a variety of factors including genetic background, diet, cultural habits and socioeconomic status. Here we characterized 110 healthy Mongolian adults gut microbiota by shotgun metagenomic sequencing and compared the intestinal microbiome among Mongolians, the Hans and European cohorts. The results showed that the taxonomic profile of intestinal microbiome among cohorts revealed the Actinobaceria and Bifidobacterium were the key microbes contributing to the differences among Mongolians, the Hans and Europeans at the phylum level and genus level, respectively. Metagenomic species analysis indicated that Faecalibacterium prausnitzii and Coprococcus comeswere enrich in Mongolian people which might contribute to gut health through anti-inflammatory properties and butyrate production, respectively. On the other hand, the enriched genus Collinsella, biomarker in symptomatic atherosclerosis patients, might be associated with the high morbidity of cardiovascular and cerebrovascular diseases in Mongolian adults. At the functional level, a unique microbial metabolic pathway profile was present in Mongolian's gut which mainly distributed in amino acid metabolism, carbohydrate metabolism, energy metabolism, lipid metabolism, glycan biosynthesis and metabolism. We can attribute the specific signatures of Mongolian gut microbiome to their unique genotype, dietary habits and living environment.The gut microbiota (GM) is recognized as a human co-evolutionary partner that facilitates nutritional acquisition and immune modulation, and helps maintain host homeostasis in response to profound lifestyle changes 1-3 . Researchers have delineated the structural and functional configurations of gut metagenomes in different world populations by next generation sequencing [4][5][6][7] . The functional GM layout may represent host genotypic characteristics and reflect an adaptive ecosystem response to the host diet and cultural habits.The microbiota of the Mongolian population is of particular interest to researchers, because Mongolia encompasses a uniquely wide range of environmental conditions and ethnogeographical cohorts. Moreover, the Mongol Empire was the world's largest contiguous empire, exerted a major influence that greatly enhanced the cultural exchange between Mongolia and Europe that took place during the Middle Ages, when Mongolians intermingled with various populations in the Eurasian continent. Importantly, it has been reported that genetically, more than 20% people in the world are related to Mongolians 8 . Mongolians living in pastureland, such as Khentii Province, maintains a traditional nomadic lifestyle (Fig. 1A), whereas those in urban areas, such as Ulan Bator (the capital of Mongolia) and TUW Province (the suburbs of the capital), have adopted an urban lifestyle due to rapid modernization and economic development. Chinese Mongolians living in Hohhot and Xilingol have inter-married with ethic Han Chinese, which may generate some differences fr...

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Section: Discussionsupporting

confidence: 71%

Unique Features of Ethnic Mongolian Gut Microbiome revealed by metagenomic analysis

Liu

Zhang

Wu³

et al. 2016

Sci Rep

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“…Studies have also shown the benefits of the uptake of adequate amounts of Se due to its anticarcinogenic, antimutagenic, antioxidative, antipathogenic, and antiinflammatory properties (Shen et al, 2010;Fairweather-Tait et al, 2011;Ren et al, 2011;Rayman, 2012;Kieliszek and Błazejak, 2013;Yazdi et al, 2013;Alfthan et al, 2015). Discoveries regarding the importance of Se for human health have aroused interest in research involving the bioaccumulation of this trace element in different LAB species with probiotic potential and their use in creating enriched and functional foods (Fisinin et al, 2009;Alzate et al, 2010;Pieniz et al, 2011;Palomo et al, 2014;Vodnar and Socaciu, 2014;Deng et al, 2015;Pusztahelyi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Assessment of selenium bioaccumulation in lactic acid bacteria

Mörschbächer

Dullius

et al. 2018

Journal of Dairy Science

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Selenium is an essential micronutrient for living beings, as it helps to maintain the normal physiological functions of the organism. The numerous discoveries involving the importance of this element to the health of human beings have fostered interest in research to develop enriched and functional foods. The present study evaluated the potential for bacterial strains of Enterococcus faecalis (CH121 and CH124), Lactobacillus parabuchneri (ML4), Lactobacillus paracasei (ML13, ML33, CH135, and CH139), and Lactobacillus plantarum (CH131) to bioaccumulate Se in their biomass by adding different concentrations of sodium selenite (30 to 200 mg/L) to the culture medium. Quantification of Se with UV and visible molecular absorption spectroscopy showed that the investigated bacteria were able to bioaccumulate this micromineral into their biomass. Two of the L. paracasei strains (ML13 and CH135) bioaccumulated the highest Se concentrations (38.1 ± 1.7 mg/g and 40.7 ± 1.1 mg/g, respectively) after culture in the presence of 150 mg/L of Se. This bioaccumulation potential has applications in the development of dairy products and may be an alternative Se source in the diets of humans and other animals.

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“…The dry weight of the bacteria is 400 μg/g and the organic selenium conversion rate is 80%.The highest conversion rate of strains studied by Ghezzi, Karathanasis, Matocha, Unrine, and Thompson (2014) was 76.5%, while the conversion rate of inorganic selenium by Bifidobacterium studied by Jin, Zhang, and Ping‐La (2009) was only 28%. Bacterial cells are less tolerant to selenite stress and the ability to reduce high concentrations of selenite to organic selenium is limited (Pusztahelyi, Kovács, Pócsi, & Prokisch, 2009). In this study, the strain of L. rhamnosus L20 had the highest conversion rate of inorganic Se of 87.65%.…”

Section: Discussionmentioning

confidence: 99%

Screening, identification, and application of selenium‐enriched Lactobacillus in goat milk powder and tablet

Shu

Mei

Chen

et al. 2020

J Food Process Preserv

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In this study, Lactobacillus L20 was screened by measuring the tolerance to the inorganic selenium and the resistance to the gastrointestinal fluid of 11 Lactobacillus preserved in the laboratory. Next, the selenium‐enriched L20 was produced into the corresponding selenium‐enriched L20 goat milk powder and milk tablet. The storage stability of the prepared powder and tablet were investigated by the accelerated test. The results indicated that the conversion rate of inorganic selenium of L20 was 87.65% and the viable count was 2.925 × 108 CFU/ml. The results of 16S rDNA homology of partial sequences of molecular characterization suggested that Lactobacillus L20 was Lactobacillus rhamnosus. More notably, the inactivation rate constants and the predicted shelf life of selenium‐enriched L. rhamnosus L20 goat milk powder and milk tablet were 2.40 × 10–4 and 1.90 × 10–4 day–1 and 285 and 406 days under 25°C, respectively, exhibiting a considerable long‐term stability. Practical applications Selenium is one of the necessary microelements in human metabolism. Lactic acid bacteria can transform the inorganic selenium into organic selenium and selenium‐enriched Lactic acid bacteria can supply organic selenium and play its probiotic function. In this study, Lactobacillus rhamnosus L20 with strong selenium‐enriching ability was obtained and freeze‐dried powder was prepared. Selenium‐enriched probiotic goat milk powder and goat milk tablets were developed. The shelf life was predicted and prepared for selenium‐enriched probiotic powder. This work also provides a technical support for the subsequent industrialization of Se‐enriched probiotic goat milk powder and tablets.

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Selenite-stress selected mutant strains of probiotic bacteria for Se source production

Cited by 34 publications

References 32 publications

Unique Features of Ethnic Mongolian Gut Microbiome revealed by metagenomic analysis

Unique Features of Ethnic Mongolian Gut Microbiome revealed by metagenomic analysis

Assessment of selenium bioaccumulation in lactic acid bacteria

Screening, identification, and application of selenium‐enriched Lactobacillus in goat milk powder and tablet

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