Screening, identification, and application of selenium‐enriched Lactobacillus in goat milk powder and tablet

Abstract

In this study, Lactobacillus L20 was screened by measuring the tolerance to the inorganic selenium and the resistance to the gastrointestinal fluid of 11 Lactobacillus preserved in the laboratory. Next, the selenium‐enriched L20 was produced into the corresponding selenium‐enriched L20 goat milk powder and milk tablet. The storage stability of the prepared powder and tablet were investigated by the accelerated test. The results indicated that the conversion rate of inorganic selenium of L20 was 87.65% and the viable count was 2.925 × 108 CFU/ml. The results of 16S rDNA homology of partial sequences of molecular characterization suggested that Lactobacillus L20 was Lactobacillus rhamnosus. More notably, the inactivation rate constants and the predicted shelf life of selenium‐enriched L. rhamnosus L20 goat milk powder and milk tablet were 2.40 × 10–4 and 1.90 × 10–4 day–1 and 285 and 406 days under 25°C, respectively, exhibiting a considerable long‐term stability. Practical applications Selenium is one of the necessary microelements in human metabolism. Lactic acid bacteria can transform the inorganic selenium into organic selenium and selenium‐enriched Lactic acid bacteria can supply organic selenium and play its probiotic function. In this study, Lactobacillus rhamnosus L20 with strong selenium‐enriching ability was obtained and freeze‐dried powder was prepared. Selenium‐enriched probiotic goat milk powder and goat milk tablets were developed. The shelf life was predicted and prepared for selenium‐enriched probiotic powder. This work also provides a technical support for the subsequent industrialization of Se‐enriched probiotic goat milk powder and tablets.

Development of Selenized Lactic Acid Bacteria and their Selenium Bioaccummulation Capacity

“…In the present study, lower selenite concentrations did not accelerate the growth of either strain, unlike the effects reported in some previous studies [16,39]. Moreover, in LAB strains tested by Shu et al [14], viable counts decreased with the increase in sodium selenite concentration. In comparison, our results suggested that CCDM 144 may have a higher tolerance to sodium selenite than CCDM 922A because no reduction in viable counts was observed in the former strain even at the highest selenite concentration used (50 mg/L).…”

“…LAB also represent a promising target group for selenization [14][15][16], as these microbes produce various antimicrobial compounds such as organic acids, hydrogen peroxide, and bacteriocins, which possess antimicrobial activity by means of the different metabolic substances generated [17]. However, as Se-enriched LAB are not yet legislatively permitted for use in foods, it is necessary to obtain additional information regarding their safety, functionality, and properties.…”

Effects of Selenium- and Zinc-Enriched Lactobacillus plantarum SeZi on Antioxidant Capacities and Gut Microbiome in an ICR Mouse Model

“…The selenium concentration in the medium is highly linked to bacterial selenium level, but the growth of most bacterial isolates from the human gut can be inhibited by the addition of inorganic selenium into the medium [ 20 ]. However, some LAB strains have been reported to be capable of resisting selenium oxyanions at high concentrations during cultivation [ 21 , 22 , 23 ]. Especially, Lactobacillus plantarum has been suggested as Se-enriched lactobacilli for food applications [ 24 ].…”

Production and use of eco‐friendly selenium nanoparticles in the fortification of yoghurt

“…Finally, Shu et al. (2020) produced Se‐enriched probiotic goat milk as powder and tablets. Lactobacillus has the potential to bio‐convert Se from inorganic to organic forms, such as selenocysteine (Alzate et al., 2007; Alzate et al., 2010; Morschbacher et al., 2018; Xu et al., 2020), which is safer and has higher nutritional value and bioactivity.…”

“…The method used in this study considered encapsulated method by using skimmed milk as a carrier for Se in NPs form (Hassan et al, ,2014;Hassan et al, ,2011;Salama et al, 2015). Finally, Shu et al (2020) produced Se-enriched probiotic goat milk as powder and tablets. Lactobacillus has the potential to bio-convert Se from inorganic to organic forms, such as selenocysteine (Alzate et al, 2007;Alzate et al, 2010;Morschbacher et al, 2018;Xu et al, 2020), which is safer and has higher nutritional value and bioactivity.…”