Assessment of selenium bioaccumulation in lactic acid bacteria

Mörschbächer, Ana Paula; Dullius, Anja; Dullius, Carlos Henrique; Brandt, Cassiano Ricardo; Kuhn, Daniel; Brietzke, Débora Tairini; Kuffel, Fernando José Malmann; Etgeton, Henrique Pretto; Altmayer, Taciélen; Gonçalves, Tamara Engelmann; Schweizer, Ytan Andreine; Oreste, Eliézer Quadro; Ribeiro, Anderson Schwingel; Lehn, Daniel Neutzling; Souza, Cláucia Fernanda Volken de; Hoehne, Lucélia

doi:10.3168/jds.2018-14852

Cited by 22 publications

(12 citation statements)

References 52 publications

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“…thermophilus has not been reported. However, the critical concentration of inhibition calculated in this study is higher than those reported for some LAB (Xia et al, 2007;González-Olivares et al, 2016;Mörschbächer et al, 2018) and lower than some growth concentrations reported for some lactobacilli (Xia et al, 2007).…”

Section: Critical Concentration Of Inhibitioncontrasting

confidence: 81%

Serine-enriched minimal medium enhances conversion of selenium into selenocysteine by Streptococcus thermophilus

Castañeda‐Ovando

Segovia-Cruz

Flores-Aguilar

et al. 2019

Journal of Dairy Science

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Selenium is included in selenoprotein sequences, which participate in enzymatic processes necessary to preserve optimal health. Some lactic acid bacteria carry out the biotransformation of inorganic selenium in their metabolism. The complete biochemical mechanism of selenium biotransformation is still unknown; however, it is known that both the selenocysteine synthesis process and its subsequent incorporation into selenoproteins include serine as part of the action of seryl-RNAt synthetase. Therefore, the aim of this work was to determine the effect of serine during the biotransformation of selenium and the subsequence growth of Streptococcus thermophilus in a minimal medium. Two culture media were prepared, one enriched with the minimum inhibitory concentration of selenite (as Na 2 SeO 3 ) and the other as a mixture of the minimum inhibitory concentration of selenite and serine. The absorbed selenium concentration was measured by inductively coupled plasma, and the selenocysteine identification was performed by reverse-phase HPLC. In the second culture medium, decreases in both times, the adaptation and the logarithmic phase, were observed. According to the results, it was possible to establish that the presence of serine allowed the biotransformation of selenite into selenocysteine by Strep. thermophilus.

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Section: Critical Concentration Of Inhibitioncontrasting

confidence: 81%

Serine-enriched minimal medium enhances conversion of selenium into selenocysteine by Streptococcus thermophilus

Castañeda‐Ovando

Segovia-Cruz

Flores-Aguilar

et al. 2019

Journal of Dairy Science

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“…It was found that lactic acid bacteria cultured for 24 h in MRS medium with sodium selenite (IV) at a concentration in the range between 0.5 and 4 mg/L bound large amounts of selenium (0.17-1.89 mg/g). Mörschbächer et al [36] reported that the concentration of accumulated selenium in the biomass of two strains of Lactobacillus paracasei (ML13 and CH135) were approximately 38 and 40 mg/g in a culture exposed to 150 mg Se 4+ /L in the MRS medium. The results obtained indicate the ability of C. utilis ATCC 9950 yeast cells to accumulate selenium not only in the logarithmic growth phase, but also during the stationary phase, as evidenced by the increase in the content of this element in the biomass of the yeast cells.…”

Section: Determination Of Selenium Content In Yeastmentioning

confidence: 99%

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

et al. 2020

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Selenium is considered an essential component of all living organisms. Studies on the enrichment of yeast cells with selenium, using the ability of cell biomass to bind this element, are being reported more and more. Yeast cultures were cultivated in YPD medium enriched with Na2SeO3 salts for 72 h at 28 °C on a shaker utilizing reciprocating motion. Selenium in cell biomass was determined with the use of ICP–MS. It was observed that the addition of selenium to the experimental medium (in the range of 4–100 mg/L) increased the content of this element in the yeast cell biomass. During the extension of cultivation time, the number of yeast cells and biomass yield exhibited a decreasing trend. Based on the obtained results, it was concluded that yeast cells exhibited the ability to accumulate selenium in both logarithmic and stationary growth phases. The dose of 20 and 30 mg/L of selenium in the culture medium meets the expectations in terms of both the content of selenium bound to yeast cells (1944 ± 110.8 μg/g dry weight) under 48-h cultivation. The obtained results confirmed that the Candida utilis ATCC 9950 strain exhibits the ability to bind selenium, which means that the biomass of these yeasts may be used as a natural source of selenium in the diet of humans and animals.

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“…Those results were lower than the accumulation of calcium ions in cells of L. rhamnosus B 442. Mörschbächer et al [30], in a study on the estimation of the potential of strains of lactic acid bacteria for bioaccumulation of selenium, observed that L. paracasei (ML13 and CH135) bio-accumulated the highest concentrations of Se (38.1 ± 1.7 mg/g and 40.7 ± 1.1 mg/g, respectively) in the presence of 150 mg/l sodium selenite. The bioaccumulation results obtained were notably higher than those presented in the above paper.…”

Section: Discussionmentioning

confidence: 99%

Influence of Pulsed Electric Field on Accumulation of Calcium in Lactobacillus rhamnosus B 442

Góral¹,

Pankiewicz²,

Sujka³

et al. 2020

Journal of Microbiology and Biotechnology

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Calcium is an element that performs many important functions in the human body. A study was conducted on the use of a pulsed electric field (PEF) to enrich cells of Lactobacillus rhamnosus B 442 in calcium ions. The highest concentration of calcium ions in bacterial cells (7.30 mg/g d.m.) was obtained at ion concentration of 200 µg/ml of medium and with the use of the following PEF parameters: field strength 3.0 kV/cm, exposure time 10 min, pulse width 75 ms and 20 h of culturing after which bacteria were treated with the field. Cell biomass varied in the range from 0.09 g/g d.m. to 0.252 g/g d.m., and the total number of bacteria ranged from 10 1 0 CFU/ml to 10 1 2 CFU/ml. Microscope photographs prove that calcium ions were situated within the cells of the bacteria, and electroporation contributed to an increase in the effectiveness of the ion bioaccumulation process. Samples containing calcium and subjected to electroporation displayed intensive fluorescence. The significance of this research was the possibility of using probiotic bacteria enriched with calcium ions for the production of functional food in subsequent studies.

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Assessment of selenium bioaccumulation in lactic acid bacteria

Cited by 22 publications

References 52 publications

Serine-enriched minimal medium enhances conversion of selenium into selenocysteine by Streptococcus thermophilus

Serine-enriched minimal medium enhances conversion of selenium into selenocysteine by Streptococcus thermophilus

Accumulation of Selenium in Candida utilis Growing in Media of Increasing Concentration of this Element

Influence of Pulsed Electric Field on Accumulation of Calcium in Lactobacillus rhamnosus B 442

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