Selective targeting of skin pathobionts and inflammation with topically applied lactobacilli

Lebeer, Sarah; Oerlemans, Eline; Claes, Ingmar; Henkens, Tim; Delanghe, Lize; Wuyts, Sander; Spacova, Irina; Broek, Marianne F.L. van den; Tuyaerts, I; Wittouck, Stijn; Boeck, Ilke De; Allonsius, Camille Nina; Kiekens, Filip; Lambert, Julien

doi:10.1016/j.xcrm.2022.100521

Cited by 26 publications

(29 citation statements)

References 65 publications

(99 reference statements)

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“…Therefore, if the desired beneficial activities are dependent on a high concentration of yeast and/or a direct interaction between the yeast and the vaginal mucosa or microbiota, topical application should be considered. However, an indirect effect of the orally supplemented yeast on the vaginal microbiome, e.g., through immunomodulation (34), should not be dismissed, along with the possibility that even at low concentrations, the supplemented yeast might have a relevant beneficial effect, in accordance with the concept of keystone microbes (39) and as our group has observed in other niches (40,41).…”

Section: Discussionmentioning

confidence: 63%

Impacts of Menstruation, Community Type, and an Oral Yeast Probiotic on the Vaginal Microbiome

Oerlemans

Ahannach

Wittouck

et al. 2022

mSphere

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Section: Discussionmentioning

confidence: 63%

Impacts of Menstruation, Community Type, and an Oral Yeast Probiotic on the Vaginal Microbiome

Oerlemans

Ahannach

Wittouck

et al. 2022

mSphere

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“…Some of the Lactobacillus strains used in the current study (LGG, KCA1 and WCFS1) are known to possess both antimicrobial and immunomodulating properties, leading to the alleviation of other inflammatory diseases 22 – 24 . These properties might make these translationally useful in the management of periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms

Holm

Carvalho

Delanghe

et al. 2023

npj Biofilms Microbiomes

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Several oral diseases are characterized by a shift within the oral microbiome towards a pathogenic, dysbiotic composition. Broad-spectrum antimicrobials are often part of patient care. However, because of the rising antibiotic resistance, alternatives are increasingly desirable. Alternatively, supplying beneficial species through probiotics is increasingly showing favorable results. Unfortunately, these probiotics are rarely evaluated comparatively. In this study, the in vitro effects of three known and three novel Lactobacillus strains, together with four novel Streptococcus salivarius strains were comparatively evaluated for antagonistic effects on proximal agar growth, antimicrobial properties of probiotic supernatant and the probiotic’s effects on in vitro periodontal biofilms. Strain-specific effects were observed as differences in efficacy between genera and differences within genera. While some of the Lactobacillus candidates were able to reduce the periodontal pathobiont A. actinomycetemcomitans, the S. salivarius strains were not. However, the S. salivarius strains were more effective against periodontal pathobionts P. intermedia, P. gingivalis, and F. nucleatum. Vexingly, most of the Lactobacillus strains also negatively affected the prevalence of commensal species within the biofilms, while this was lower for S. salivarius strains. Both within lactobacilli and streptococci, some strains showed significantly more inhibition of the pathobionts, indicating the importance of proper strain selection. Additionally, some species showed reductions in non-target species, which can result in unexpected and unexplored effects on the whole microbiome.

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“…In this study, we use several key evaluation criteria for beneficial lactobacilli strains as candidates for URT application based on not only their functionality against respiratory viral disease but also considering their strain safety and applicability in the formulation of interest (Figure 1B ). Of note, similar criteria have previously allowed us to develop a multi‐strain probiotic formulation for topical skin application against acne (Lebeer et al, 2022 ). Our goal was to develop a safe and readily applicable live biotherapeutic formulation for the URT with several lactobacilli that together are potentially capable of providing multifactorial activity against respiratory viruses.…”

Section: Introductionmentioning

confidence: 94%

Development of a live biotherapeutic throat spray with lactobacilli targeting respiratory viral infections

Spacova

Boeck

Cauwenberghs

et al. 2022

Microbial Biotechnology

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Respiratory viruses such as influenza viruses, respiratory syncytial virus (RSV), and coronaviruses initiate infection at the mucosal surfaces of the upper respiratory tract (URT), where the resident respiratory microbiome has an important gatekeeper function. In contrast to gut-targeting administration of beneficial bacteria against respiratory viral disease, topical URT administration of probiotics is currently underexplored, especially for the prevention and/or treatment of viral infections. Here, we report the formulation of a throat spray with live lactobacilli exhibiting several in vitro mechanisms of action against respiratory viral infections, including induction of interferon regulatory pathways and direct inhibition of respiratory viruses. Rational selection of Lactobacillaceae strains was based on previously documented beneficial properties, up-scaling and industrial production characteristics, clinical safety parameters, and potential antiviral and immunostimulatory efficacy in the URT demonstrated in this study. Using a three-step selection strategy, three strains were selected and further tested in vitro antiviral assays and in formulations: Lacticaseibacillus casei AMBR2 as a promising endogenous candidate URT probiotic with previously reported barrier-enhancing and anti-pathogenic properties and the two well-studied model strains Lacticaseibacillus rhamnosus GG and Lactiplantibacillus plantarum WCFS1 that display immunomodulatory capacities. The three strains and their combination significantly reduced the cytopathogenic effects of RSV, influenza A/H1N1 and B viruses, and HCoV-229E coronavirus in co-culture models with bacteria, virus, and host cells. Subsequently, these strains were formulated in a throat spray and human monocytes were employed to confirm the formulation process did not reduce the interferon regulatory pathway-inducing capacity. Administration of the throat spray in healthy volunteers revealed that the lactobacilli were capable of temporary colonization of the throat in a metabolically active form. Thus, the developed spray with live lactobacilli will be further explored in the clinic as a potential broad-acting live biotherapeutic strategy against respiratory viral diseases.

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Selective targeting of skin pathobionts and inflammation with topically applied lactobacilli

Cited by 26 publications

References 65 publications

Impacts of Menstruation, Community Type, and an Oral Yeast Probiotic on the Vaginal Microbiome

Impacts of Menstruation, Community Type, and an Oral Yeast Probiotic on the Vaginal Microbiome

Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms

Development of a live biotherapeutic throat spray with lactobacilli targeting respiratory viral infections

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