Impacts of Menstruation, Community Type, and an Oral Yeast Probiotic on the Vaginal Microbiome

Oerlemans, Eline; Ahannach, Sarah; Wittouck, Stijn; Dehay, Elodie; Boeck, Ilke De; Ballet, Nathalie; Rodríguez, B. Rodríguez; Tuyaerts, I; Lebeer, Sarah

doi:10.1128/msphere.00239-22

Cited by 10 publications

(12 citation statements)

References 51 publications

(81 reference statements)

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“…Importantly, we did not observe significant antagonistic activity between S. cerevisiae CNCM I-3856 and the tested lactobacilli regarding C. albicans inhibition, highlighting that they can be applied together without loss of activity. Furthermore, it is plausible that S. cerevisiae CNCM I-3856 will not have major antagonistic effects with the lactobacilli comprising the natural vaginal microbiome, as we have shown for the vaginal lactobacilli in this paper, and recently demonstrated in healthy women 14 . This is also relevant for oral S. cerevisiae CNCM I-3856 administration, as it has previously been demonstrated that after its oral administration in women S. cerevisiae CNCM I-3856 can be recovered from vaginal samples of at least 18% to 21% of participants, depending on the analysis 14 , 27 .…”

Section: Discussionsupporting

confidence: 54%

“…Several studies show that oral (as a food supplement) and vaginal (generally as a drug, with a more complex regulatory route) intake of different probiotic species and strains is associated with improved clinical signs 11 – 13 . In addition, no side effects have been observed or reported upon probiotic use in healthy women 14 or women suffering from VVC 11 , 15 , 16 .…”

Section: Introductionmentioning

confidence: 96%

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Multifactorial inhibition of Candida albicans by combinations of lactobacilli and probiotic Saccharomyces cerevisiae CNCM I-3856

Spacova,

Allonsius,

De Boeck

et al. 2024

Sci Rep

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Strategies against the opportunistic fungal pathogen Candida albicans based on probiotic microorganisms represent a promising alternative to traditional antifungals. Here, we investigated the effects of Lactobacillaceae isolates from fermented foods or the human vagina, alone or in combination with the probiotic yeast Saccharomyces cerevisiae CNCM I-3856, against C. albicans in vitro. Nine out of nineteen tested strains of Lactobacillaceae inhibited growth of C. albicans with inhibition zones of 1–3 mm in spot assays. Five out of nineteen lactobacilli tested as such or in combination with S. cerevisiae CNCM I-3856 also significantly inhibited C. albicans hyphae formation, including Limosilactobacillus fermentum LS4 and L. fermentum LS5 resulting in respectively 62% and 78% hyphae inhibition compared to the control. Thirteen of the tested nineteen lactobacilli aggregated with the yeast form of C. albicans, with Lactiplantibacillus carotarum AMBF275 showing the strongest aggregation. The aggregation was enhanced when lactobacilli were combined with S. cerevisiae CNCM I-3856. No significant antagonistic effects were observed between the tested lactobacilli and S. cerevisiae CNCM I-3856. The multifactorial activity of Lactobacillaceae strains alone or combined with the probiotic S. cerevisiae CNCM I-3856 against C. albicans without antagonistic effects between the beneficial strains, paves the way for developing consortium probiotics for in vivo applications.

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Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 96%

Multifactorial inhibition of Candida albicans by combinations of lactobacilli and probiotic Saccharomyces cerevisiae CNCM I-3856

Spacova,

Allonsius,

De Boeck

et al. 2024

Sci Rep

Self Cite

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“…During menses, increased diversity (Krog et al, 2022) and decreased Lactobacillus abundances (Song et al, 2020) were observed. During the follicular and luteal phases, the abundance of Lactobacillus is expanded, and the vaginal microbiome of more women becomes dominated by Lactobacillus due to the increase of estradiol (Gajer et al, 2012;Song et al, 2020;Jie et al, 2021;Krog et al, 2022;Oerlemans et al, 2022). In postmenopausal women, species richness was decreased, but species diversity was significantly increased (Kim et al, 2021).…”

Section: Normal Cervicovaginal Microbiomementioning

confidence: 99%

Interactions between microbiota and cervical epithelial, immune, and mucus barrier

Dong

Bai

et al. 2023

Front. Cell. Infect. Microbiol.

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The female reproductive tract harbours hundreds of bacterial species and produces numerous metabolites. The uterine cervix is located between the upper and lower parts of the female genital tract. It allows sperm and birth passage and hinders the upward movement of microorganisms into a relatively sterile uterus. It is also the predicted site for sexually transmitted infection (STI), such as Chlamydia, human papilloma virus (HPV), and human immunodeficiency virus (HIV). The healthy cervicovaginal microbiota maintains cervical epithelial barrier integrity and modulates the mucosal immune system. Perturbations of the microbiota composition accompany changes in microbial metabolites that induce local inflammation, damage the cervical epithelial and immune barrier, and increase susceptibility to STI infection and relative disease progression. This review examined the intimate interactions between the cervicovaginal microbiota, relative metabolites, and the cervical epithelial-, immune-, and mucus barrier, and the potent effect of the host-microbiota interaction on specific STI infection. An improved understanding of cervicovaginal microbiota regulation on cervical microenvironment homeostasis might promote advances in diagnostic and therapeutic approaches for various STI diseases.

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“…4 On the other hand, L. iners seems to resist and dominate the vaginal environment when in the presence of microbiota disturbances such as menstruation. 23,24 However, it should be considered that CSTIII has been associated with several sexually transmitted infections 25,26 because infections caused by C. trachomatis and by human papillomavirus (HPV) are reinforcing the concerns about the protective role of L. iners in vaginal microbiota. The findings of the current study show that women with CSTIII who include milk and/or dairy products in their diet have increased L. crispatus RAs.…”

Section: Discussionmentioning

confidence: 99%

“…The CSTIII has been associated with a higher temporal instability because it can alternate to CSTIV and vice-versa in a short period 4 . On the other hand, L. iners seems to resist and dominate the vaginal environment when in the presence of microbiota disturbances such as menstruation 23,24 . However, it should be considered that CSTIII has been associated with several sexually transmitted infections 25,26 because infections caused by C. trachomatis and by human papillomavirus (HPV) are reinforcing the concerns about the protective role of L. iners in vaginal microbiota.…”

Section: Discussionmentioning

confidence: 99%

Milk and Dairy Consumption and Its Relationship With Abundance of Lactobacillus crispatus in the Vaginal Microbiota: Milk Intake and Vaginal Lactobacillus

Moura

Silva

Marconi

2023

J Low Genit Tract Dis

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ObjectivesDiet habits, such as low milk and dairy intake, have been associated with bacterial vaginosis. Thus, the authors compared vaginal Lactobacillus crispatus abundances in women with different molecularly defined community state types (CSTs) according to the consumption of milk and/or dairy products.MethodsA total of 516 women from the 5 geographic regions of Brazil were included. Participants were interviewed with a structured questionnaire for assessment of milk and/or dairy intake. Vaginal samples were used for sequencing of V3-V4 regions of the 16S ribosomal RNA gene for further determination of L. crispatus relative abundance (RA) and clustering into 1 of the 5 CSTs (CSTI–CSTV), as firstly described by Ravel et al. (2011). The nonparametric Mann-Whitney test was used to compare L. crispatus RA within the most representative CSTs (L. crispatus-dominant CSTI, Lactobacillus iners-dominant CSTIII, and Lactobacillus-depleted CSTIV) in this population, according to the frequency of milk and/or dairy intake.ResultsThe prevalence of CSTI was 33.3% (n = 172), CSTIII was 39% (n = 201), and CSTIV was 27.7% (n = 143). Among the participants with CSTIII, higher L. crispatus RA was observed for those who reported milk/dairy intake (median = 0.02; interquartile range = 0.01–0.09) than those with no consumption (median = 0.01; interquartile range = 0–0.03) (p = .03). Such difference was not observed for participants with CSTI and CSTIV.ConclusionsWomen with vaginal microbiota dominated by L. iners who consume milk and/or dairy present increased abundances of L. crispatus. Therefore, they could benefit from L. crispatus protective properties conferring greater temporal microbiota stability and, consequently, increased protection against infections.

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Impacts of Menstruation, Community Type, and an Oral Yeast Probiotic on the Vaginal Microbiome

Abstract: How to define and promote a healthy state of the vaginal microbiome is not well understood. Knowledge of which underlying factors shape the microbial community composition of the vagina and how to modulate them will contribute to vaginal disease prevention and improve fertility.

Cited by 10 publications

References 51 publications

Multifactorial inhibition of Candida albicans by combinations of lactobacilli and probiotic Saccharomyces cerevisiae CNCM I-3856

Multifactorial inhibition of Candida albicans by combinations of lactobacilli and probiotic Saccharomyces cerevisiae CNCM I-3856

Interactions between microbiota and cervical epithelial, immune, and mucus barrier

Milk and Dairy Consumption and Its Relationship With Abundance of Lactobacillus crispatus in the Vaginal Microbiota: Milk Intake and Vaginal Lactobacillus

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