Selective GLC Determination of Epinephrine, Isoproterenol, and Phenylephrine in Pharmaceutical Dosage Forms

Watson, James R.; Lawrence, R. C.

doi:10.1002/jps.2600660426

Cited by 27 publications

(3 citation statements)

References 24 publications

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“…It can also be an effective cardiac stimulant for the prevention of heart block and in the treatment of cardiac arrhythmias. [14] Electrochemical procedures [15 -17] and flow injection procedures [3 -5,12] for the determination of isoproterenol have also been reported. The isoproterenol is better absorbed when dispensed by inhalation.…”

Section: Isoproterenol or Isoprenaline 4-[1-hydroxy-2-[(1-methylethylmentioning

confidence: 99%

Flow Injection Spectrophotometric Determination of Isoproterenol with an On‐Line Solid‐Phase Reactor Containing Immobilized Manganese Dioxide

2004

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This work reports the application of a simple, sensitive, and rapid flow injection system for the spectrophotometric determination of isoproterenol by using a solid-phase reactor containing MnO 2 (s) immobilized in a polyester resin. The method is based on the oxidation of isoproterenol to produce isoproterochrome, which was monitored at 492 nm. The analytical curve was linear in the isoproterenol concentration range from 1.0 Â 10 25 -2.0 Â 10 24 mol L 21 , with a detection limit of 1.7 Â 10 26 mol L 21 . The relative standard deviation (RSD) was 0.9%,

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Section: Isoproterenol or Isoprenaline 4-[1-hydroxy-2-[(1-methylethylmentioning

confidence: 99%

Flow Injection Spectrophotometric Determination of Isoproterenol with an On‐Line Solid‐Phase Reactor Containing Immobilized Manganese Dioxide

2004

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“…2 It is mostly used to treat bradycardia, heart block, chronic obstructive pulmonary diseases and rare in asthma, and as an active bronchodilator. [3][4][5] The literature review on availability of analytical methods to identify isoproterenol hydrochloride and its metabolites in biological samples such as high performance liquid chromatography, liquid chromatography-mass spectrometry (LC-MS) 6,7 gas-liquid chromatography 8 and poly vinylchloride membrane selective electrode 9 , selective separation using bismuth silicate ion-exchanger 10 , chemiluminescence determination using luminol-diperiodatoargentate(III) 11 and characterization of new process related impurity using HPLC, LC/ESI-MS, NMR. 12 A sensitive spectrophotometric method recently has been developed for the determination of isopropyl amine, a core moiety of isoproterenol hydrochloride, at the trace level in pharmaceutical drug substances.…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating Method Development and Validation for the Quantification of Isoproterenol HCL in Bulk and Its Formulation by Rp-HPLC Using Pda Detection

Muneer¹,

Ahad²,

Bonnoth³

2018

J. Pharm. Sci. Innov.

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The objective of the present study was to develop an accurate, precise stability indicating reverse phase liquid chromatographic method to quantify Isoproterenol HCl in bulk and its formulation using PDA detection. The chromatographic separation of the analyte from the degradants was achieved on Phenomenex Luna column with mobile phase composition of Methanol and 0.1% Triethyl amine [pH 7.0], [20:80% v/v] at flow rate of 1.0 ml/min. The analyte separation was monitored using PDA detection at 279nm. The method was linear in the concentration range of 10-60 μg/ml. The method shown acceptable percent relative deviations for the Inter-day (0.58%) and Intra-day (0.65%) precisions. The mean percent recovery for accuracy study was within the limit. From the stability studies method has proven specificity to quantify Isoproterenol HCl in presence of its degradation products. The method shown acceptance as according to ICH guidelines.

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“…Several methods have been reported to identify isoproterenol hydrochloride and its metabolites in biological samples such as high performance liquid chromatography (HPLC), liquid chromatography–mass spectrometry (LC–MS) [5] , [6] , gas-liquid chromatography (GLC) [7] and poly(vinylchloride) (PVC) membrane selective electrode [8] , selective separation using bismuth silicate ion-exchanger [9] and chemiluminescence determination using luminol-diperiodatoargentate(III) [10] . A sensitive spectrophotometric method recently has been developed for the determination of isopropyl amine, a core moiety of isoproterenol hydrochloride, at the trace level in pharmaceutical drug substances [11] .…”

Section: Introductionmentioning

confidence: 99%

Synthesis, isolation, identification and characterization of new process-related impurity in isoproterenol hydrochloride by HPLC, LC/ESI-MS and NMR

Kumar

Devineni

Gajjala

et al. 2017

Journal of Pharmaceutical Analysis

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One unknown impurity (Imp-II) during the analysis of laboratory batches of isoproterenol hydrochloride was detected in the level ranging from 0.04% to 0.12% by high performance liquid chromatography with UV detection. The unknown impurity structure was proposed as 4-[2-(propan-2-ylamino)ethyl]benzene-1,2-diol (Imp-II) using the liquid chromatography--mass spectrophotometry (LC--MS) analysis. Imp-II was isolated by semi-preparative liquid chromatography from the impurity-enriched reaction crude sample. Its proposed structure was confirmed by nuclear magnetic spectroscopy such as 1H, 13C, DEPT (1D NMR), HSQC (2D NMR) and infrared spectroscopy (IR), and retention time and purity with HPLC followed by the chemical synthesis. Due to less removable nature of Imp-II during the purification, the synthetic process was optimized proficiently to control the formation of Imp-II below to the limit<0.12% in the course of reaction. The new chemical route was developed for the preparation of this impurity in required quantity with purity to use as reference standard. The most probable mechanism for the formation of Imp-II was discussed in details.

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Selective GLC Determination of Epinephrine, Isoproterenol, and Phenylephrine in Pharmaceutical Dosage Forms

Cited by 27 publications

References 24 publications

Flow Injection Spectrophotometric Determination of Isoproterenol with an On‐Line Solid‐Phase Reactor Containing Immobilized Manganese Dioxide

Flow Injection Spectrophotometric Determination of Isoproterenol with an On‐Line Solid‐Phase Reactor Containing Immobilized Manganese Dioxide

Stability Indicating Method Development and Validation for the Quantification of Isoproterenol HCL in Bulk and Its Formulation by Rp-HPLC Using Pda Detection

Synthesis, isolation, identification and characterization of new process-related impurity in isoproterenol hydrochloride by HPLC, LC/ESI-MS and NMR

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