Selective enrichment of aminothiols using polysorbate 20-capped gold nanoparticles followed by capillary electrophoresis with laser-induced fluorescence

Shen, Chien‐Chih; Tseng, Wei‐Lung; Hsieh, Ming‐Mu

doi:10.1016/j.chroma.2008.11.044

Cited by 60 publications

(43 citation statements)

References 30 publications

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“…For example, Cys deficiency correlates with slow growth, hair depigmentation, edema, and other body disorders , while Hcy is highly associated with cardiovascular and Alzheimer's disease . Currently determination of these thiols can be achieved by gas chromatography with mass spectroscopy (Huang and Chang, 2006;Sass and Endres, 1997), high performance liquid chromatography (Cao et al, 2004;Lu et al, 2007), or capillary electrophoresis Shen et al, 2009) in conjunction with fluorimetric/electrochemical detector. However, these methods suffer from poor reproducibility, requirement of expensive and sophisticated instrumentation or complicated sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma

Yin

et al. 2009

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma

Yin

et al. 2009

Biosensors and Bioelectronics

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“…Shen et al [90] reported an interesting method for the selective extraction of thiols, using gold nanoparticles (AuNPs), as a result of the formation of Au-S bonds. The AuNPs were coupled with non-ionic surfactant Tween 20, providing high selectivity for aminothiols with respect to previously reported use of AuNPs capped with citrate [91].…”

Section: Fluorescence Detectionmentioning

confidence: 99%

Capillary electrophoresis in the evaluation of aminothiols in body fluids

Carlucci

Tabucchi

2009

Journal of Chromatography B

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“…The research groups of Xu et al and Zhang et al have prepared monolithic capillary columns modified with AuNPs for the selective capture of cysteine‐containing peptides from the mixtures . Some other groups used AuNPs to selectively extract thiol‐containing compounds in human urine and plasma …”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry combined with affinity probes for the identification of CP4 EPSPS in genetically modified soybeans

Devi

Chu

et al. 2019

J Mass Spectrom

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Sample preparation methods used for genetically modified organisms (GMOs) analysis are often time consuming, require extensive manual manipulation, and result in limited amounts of purified protein, which may complicate the detection of low‐abundance GM protein. A robust sample pretreatment method prior to mass spectrometry (MS) detection of the transgenic protein (5‐enolpyruvylshikimate‐3‐phosphate synthase [CP4 EPSPS]) present in Roundup Ready soya is investigated. Liquid chromatography‐multiple reaction monitoring tandem MS (nano LC‐MS/MS‐MRM) was used for the detection and quantification of CP4 EPSPS. Gold nanoparticles (AuNPs) and concanavalin A (Con A)‐immobilized Sepharose 4B were used as selective probes for the separation of the major storage proteins in soybeans. AuNPs that enable the capture of cysteine‐containing proteins were used to reduce the complexity of the crude extract of GM soya. Con A‐sepharose was used for the affinity capture of β‐conglycinin and other glycoproteins of soya prior to enzymatic digestion. The methods enabled the detection of unique peptides of CP4 EPSPS at a level as low as 0.5% of GM soya in MRM mode. Stable‐isotope dimethyl labeling was further applied to the quantification of GM soya. Both probes exhibited high selectivity and efficiency for the affinity capture of storage proteins, leading to the quantitative detection at 0.5% GM soya, which is a level below the current European Union's threshold for food labeling. The square correlation coefficients were greater than 0.99. The approach for sample preparation is very simple without the need for time‐consuming protein prefractionation or separation procedures and thus presents a significant improvement over existing methods for the analysis of the GM soya protein.

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Selective enrichment of aminothiols using polysorbate 20-capped gold nanoparticles followed by capillary electrophoresis with laser-induced fluorescence

Cited by 60 publications

References 30 publications

Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma

Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma

Capillary electrophoresis in the evaluation of aminothiols in body fluids

Mass spectrometry combined with affinity probes for the identification of CP4 EPSPS in genetically modified soybeans

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