Capillary electrophoresis in the evaluation of aminothiols in body fluids

Carlucci, Filippo; Tabucchi, Antonella

doi:10.1016/j.jchromb.2009.07.030

Cited by 53 publications

(39 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The choice of the derivatization reagent is important not only for the sensitive detection but also for the stabilization of thiols, improvement of chromatographic properties and ionization responses (electrospray ionization-mass spectrometry), or introduction of a charge (CE). Several reviews on analytical methods for thiols including those with derivatization step were published in recent years [1][2][3][4][5][6][7][8][9]. In this report application of ultraviolet derivatization reactions in HPLC and CE analysis of hydrophilic thiols are reviewed.…”

Section: Need For Derivatizationmentioning

confidence: 99%

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Kuśmierek

Chwatko

Głowacki

et al. 2011

Journal of Chromatography B

111

View full text Add to dashboard Cite

Section: Need For Derivatizationmentioning

confidence: 99%

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Kuśmierek

Chwatko

Głowacki

et al. 2011

Journal of Chromatography B

111

View full text Add to dashboard Cite

“…[1][2][3] Many approaches based on highperformance liquid chromatography (HPLC) and capillary electrophoresis (CE) have been developed to determine these aminothiols. [4][5][6][7] Because aminothiols do not contain chromophore groups, derivatization with fluorescent detection is involved in many methods. A few rather sensitive HPLC approaches (LOD < 1 μM) were also proposed in which the application of reagents, such as 2-chloro-1-methylquinoline tetrafluoroborate (CMQT), 8 1,1′-thiocarbonyldiimidazole (TCDI), 9 4,4′-dithiodipyridine 10 and 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) 11 made it possible to use conventional UV-detectors.…”

Section: Introductionmentioning

confidence: 99%

Determination of Blood Plasma Aminothiols Using Derivatization-enhanced Capillary Transient Isotachophoresis

et al. 2018

View full text Add to dashboard Cite

Experimental Reagents and chemicalsAcetonitrile (ACN; 99.9%; Lab-Scan, Gliwice, Poland); CHCl3 (>99.8% for LC; Merck, Darmstadt, Germany); L-Cys 97%, dimethyl sulfoxide (DMSO, >99.8%), dithiothreitol (DTT, >99%), DTNB, formic acid 98%, D,L-Hcy >95%, GSH reduced >98%, N-(2-mercaptopropionyl)glycine (MPG), NaCl 99.5%, A sensitive capillary electrophoresis method was developed for the determination of aminothiol (cysteine, homocysteine, and glutathione) total levels in human blood plasma. Analytes were derivatized with Ellman's reagent (5,5′-dithiobis(2-nitrobenzoic acid)) after reduction with dithiothreitol. Liquid-liquid extraction was applied to purify the samples and concentrate the analytes. Total analysis time was 7.5 min using a silica capillary (50 μm i.d.; effective separation length 23.5 cm). Electrophoretic separation was performed using 50 mM citric acid with 20 mM triethanolamine (pH 3) containing 2% Ficoll 400. Detection limit was 0.8 μM for glutathione and 0.3 μM for both cysteine and homocysteine. Accuracy was 94 -107%, repeatability and reproducibility were ca. 2.7 -3.5 and 2.5 -6.5%, respectively.

show abstract

“…Past attempts have been made to detect the amino acids through sophisticated analytical methods like gas chromatography, high-performance liquid chromatography (HPLC), or through simpler techniques like capillary electrophoresis, electrochemistry, spectrofluorimetry and chemiluminescence [6,7]. However, these techniques have several shortcomings like less cost effectiveness, involvement of hefty lab procedures, and cross reactivity with the other amino acids.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Citrate-Capped Gold Nanoparticles with the Selected Amino Thiols for Sensing Applications

Rajeshwari

Garg

Elavarasi

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

View full text Add to dashboard Cite

The amino thiols, methionine, cysteine, and homocysteine are considered to be important biomarkers for various diseases. In the present study, the authors have demonstrated that the interactions of synthesized citratecapped gold nanoparticles with these amino thiols at different pH values can be used to develop simple colorimetric methods for their detection in aqueous solutions. The aggregation of the gold nanoparticles by interactions with the thiol group was found to depend strongly on the pH of the amino thiols. At pH 10, the detection of cysteine was possible in the presence of homocysteine at low concentration and similarly homocysteine could be detected in the presence of cysteine at low concentration by thiol-induced aggregation of the gold nanoparticles. Cysteine and methionine were specifically detected by monitoring the aggregation of the gold nanoparticles through the UV-Visible spectra at pH 7 and pH 10 respectively. The increase in the mean hydrodynamic diameter of the particles confirmed the thiol-induced aggregation of the particles both at pH 7 and pH 10. The specific pH-based interactions can form a basis to design simple colorimetric sensors for the amino thiols using as-synthesized citrate-capped gold nanoparticles without further functionalization.

show abstract

Capillary electrophoresis in the evaluation of aminothiols in body fluids

Cited by 53 publications

References 98 publications

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Determination of Blood Plasma Aminothiols Using Derivatization-enhanced Capillary Transient Isotachophoresis

Interaction of Citrate-Capped Gold Nanoparticles with the Selected Amino Thiols for Sensing Applications

Contact Info

Product

Resources

About