Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma

Nanoparticles in Biomedical Applications and Their Safety Concerns

“…Gold nanoparticles have been the popular choice for near IR emitting nano fluorophores since it is relative biocompatible and easy to synthesize. (Lee, Cha et al 2008;Shang, Yin et al 2009) The surface plasmon resonance is dependent on the size of the nanoparticles that it moves towards red with increasing particle size. Other types of gold nanomaterials such as gold nanorods and gold nanoshells were also popularly used in bioimaging because of its tunable surface plasmon bands and controllable position of the resonance by varying the synthesis conditions.…”

DNA-stabilized bimetallic nanozyme and its application on colorimetric assay of biothiols

“…500 µL human serum samples were firstly incubated with 40 µL HCl (0.2 M) and 20 µL triphenylphosphine (0.4 M) for 15 min, and then mixed with 500 µL acetonitrile for 2 h to precipitate proteins according to previously methods (Shang et al 2009;Jia et al 2015). The obtained supernatant was collected by centrifugation at 4000 rpm for 20 min, and further filtered through a filter with a pore size of 0.2 μm.…”

Sensitive turn-on fluorescence assay of methimazole based on the fluorescence resonance energy transfer between acridine orange and silver nanoparticles

“…As can be seen thiol-containing amino acids such as cysteine have the most remarkable interference effect on the system. However, most of the aminothiols are bound to proteins or other amino acids in the form of disulfide that cannot interfere as long as they do not undergo hydrolysis [40][41][42]. Furthermore, the interference of proteins is simply removed by their precipitation by acetonitrile.…”