Selective antimicrobial activity of cell lytic enzymes in a bacterial consortium

Bhagwat, Amala; Collins, Cynthia H.; Dordick, Jonathan S.

doi:10.1007/s00253-019-09955-0

Cited by 21 publications

(11 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While the latter can be well solved by lysostaphin deimmunization, the former may be overcome by enzyme engineering, coadministration of certain adjuvants, or highly efficient targeted delivery of the enzyme. Second, the enzyme may be degraded by proteases. , This receives little attention and is worth investigation. Third, the peptidoglycan structure of S. aureus may be modified in vivo , rendering it less susceptible to lysostaphin .…”

Section: Limitations and Future Perspectivesmentioning

confidence: 99%

Lysostaphin: Engineering and Potentiation toward Better Applications

Zha

Zheng

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Lysostaphin is a potent bacteriolytic enzyme with endopeptidase activity against the common pathogen Staphylococcus aureus. By digesting the pentaglycine crossbridge in the cell wall peptidoglycan of S. aureus including the methicillin-resistant strains, lysostaphin initiates rapid lysis of planktonic and sessile cells (biofilms) and has great potential for use in agriculture, food industries, and pharmaceutical industries. In the past few decades, there have been tremendous efforts in potentiating lysostaphin for better applications in these fields, including engineering of the enzyme for higher potency and lower immunogenicity with longer-lasting effects, formulation and immobilization of the enzyme for higher stability and better durability, and recombinant expression for low-cost industrial production and in situ biocontrol. These achievements are extensively reviewed in this article focusing on applications in disease control, food preservation, surface decontamination, and pathogen detection. In addition, some basic properties of lysostaphin that have been controversial and only elucidated recently are summarized, including the substrate-binding properties, the number of zinc-binding sites, the substrate range, and the cleavage site in the pentaglycine crossbridge. Resistance to lysostaphin is also highlighted with a focus on various mechanisms. This article is concluded with a discussion on the limitations and future perspectives for the actual applications of lysostaphin.

show abstract

Section: Limitations and Future Perspectivesmentioning

confidence: 99%

Lysostaphin: Engineering and Potentiation toward Better Applications

Zha

Zheng

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…etc., were found to be involved in the biosynthesis of IAA through the formation of indole-3-pyruvic acid and indole-3-acetic aldehyde [59][60][61][62][63]. Growth improvement through enzyme activity such as proteases, chitinases, cellulases, phosphatases, etc., is another mechanism used by plant growth-promoting (PGP) bacteria via attacking pathogens through the excretion of cell wall degenerating enzymes [56,[64][65][66][67]. KSB (Grz) isolate was noteworthy as it showed outstanding performance regarding the solubilization of insoluble K, and can be utilized as a K biofertilizer in colder regions.…”

Section: Production Of Plant Growth-promoting Substancesmentioning

confidence: 99%

Psychrotolerant Mesorhizobium sp. Isolated from Temperate and Cold Desert Regions Solubilizes Potassium and Produces Multiple Plant Growth Promoting Metabolites

et al. 2021

View full text Add to dashboard Cite

Soil potassium (K) supplement depends intensively on the application of chemical fertilizers, which have substantial harmful environmental effects. However, some bacteria can act as inoculants by converting unavailable and insoluble K forms into plant-accessible forms. Such bacteria are an eco-friendly approach for enhancing plant K absorption and consequently reducing utilization of chemical fertilization. Therefore, the present research was undertaken to isolate, screen, and characterize the K solubilizing bacteria (KSB) from the rhizosphere soils of northern India. Overall, 110 strains were isolated, but only 13 isolates showed significant K solubilizing ability by forming a halo zone on solid media. They were further screened for K solubilizing activity at 0 °C, 1 °C, 3 °C, 5 °C, 7 °C, 15 °C, and 20 °C for 5, 10, and 20 days. All the bacterial isolates showed mineral K solubilization activity at these different temperatures. However, the content of K solubilization increased with the upsurge in temperature and period of incubation. The isolate KSB (Grz) showed the highest K solubilization index of 462.28% after 48 h of incubation at 20 °C. The maximum of 23.38 µg K/mL broth was solubilized by the isolate KSB (Grz) at 20 °C after 20 days of incubation. Based on morphological, biochemical, and molecular characterization (through the 16S rDNA approach), the isolate KSB (Grz) was identified as Mesorhizobium sp. The majority of the strains produced HCN and ammonia. The maximum indole acetic acid (IAA) (31.54 µM/mL) and cellulase (390 µM/mL) were produced by the isolate KSB (Grz). In contrast, the highest protease (525.12 µM/mL) and chitinase (5.20 µM/mL) activities were shown by standard strain Bacillus mucilaginosus and KSB (Gmr) isolate, respectively.

show abstract

“…Dispersin B is a biofilmreleasing enzyme from Aggregatibacter actinomycetemcomitans and has been used to eliminate biofilms in pig skin infected by S. epidermidis and S. aureus [73]. Lysostaphin is a glycylglycine endopeptidase bacteriocin that cleaves the pentaglycine cross-bridge in Staphylococcal peptidoglycan for disorganization of the biofilm matrix [74].…”

Section: Treatment Strategies For Biofilm Eradicationmentioning

confidence: 99%

The Antibiofilm Nanosystems for Improved Infection Inhibition of Microbes in Skin

et al. 2021

View full text Add to dashboard Cite

Biofilm formation is an important virulence factor for the opportunistic microorganisms that elicit skin infections. The recalcitrant feature of biofilms and their antibiotic tolerance impose a great challenge on the use of conventional therapies. Most antibacterial agents have difficulty penetrating the matrix produced by a biofilm. One novel approach to address these concerns is to prevent or inhibit the formation of biofilms using nanoparticles. The advantages of using nanosystems for antibiofilm applications include high drug loading efficiency, sustained or prolonged drug release, increased drug stability, improved bioavailability, close contact with bacteria, and enhanced accumulation or targeting to biomasses. Topically applied nanoparticles can act as a strategy for enhancing antibiotic delivery into the skin. Various types of nanoparticles, including metal oxide nanoparticles, polymeric nanoparticles, liposomes, and lipid-based nanoparticles, have been employed for topical delivery to treat biofilm infections on the skin. Moreover, nanoparticles can be designed to combine with external stimuli to produce magnetic, photothermal, or photodynamic effects to ablate the biofilm matrix. This study focuses on advanced antibiofilm approaches based on nanomedicine for treating skin infections. We provide in-depth descriptions on how the nanoparticles could effectively eliminate biofilms and any pathogens inside them. We then describe cases of using nanoparticles for antibiofilm treatment of the skin. Most of the studies included in this review were supported by in vivo animal infection models. This article offers an overview of the benefits of nanosystems for treating biofilms grown on the skin.

show abstract

Selective antimicrobial activity of cell lytic enzymes in a bacterial consortium

Cited by 21 publications

References 58 publications

Lysostaphin: Engineering and Potentiation toward Better Applications

Lysostaphin: Engineering and Potentiation toward Better Applications

Psychrotolerant Mesorhizobium sp. Isolated from Temperate and Cold Desert Regions Solubilizes Potassium and Produces Multiple Plant Growth Promoting Metabolites

The Antibiofilm Nanosystems for Improved Infection Inhibition of Microbes in Skin

Contact Info

Product

Resources

About