Selection of potential Indonesian plant species for antioxidant

Abstract

Diabetic foot ulcer (DFU) is commonly complication of diabetes mellitus. Antimicrobial and antioxidant herbs can be used as complementary therapy in DFU and these properties will be strengthened by silver nanoparticles (AgNPs). This study was performed for screening of Indonesian herbs promising as antioxidant which can be subsequently involved in the green synthesis of AgNPs. Antioxidant of eight herbs extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl method. Their total group of compounds were quantified as well. Phyllanthus niruri and Orthosiphon stamineus exhibited the strongest antioxidant power with IC50 of 102 and 133 μg/ml, followed by Curcuma domestica, Stelechocarpus burahol, and Curcuma xanthorriza with the IC50 of 363, 481, and 540 μg/ml, respectively. Sonchus arvensis, Apium graveolens, Centella asiatica did not have antioxidants activity. Total phenolics from the highest is: O. stamineus > C. domestica > C. xanthorrizha > P. niruri > S. burahol > S. arvensis > A. graveolens > C. asiatica, whereas total flavonoids order is O. stamineus > S. burahol > P. niruri > A. graveolens > C. asiatica > S. arvensis > C. xanthorrizha > C. domestica. We concluded that O. stamineus was the most suitable herb to be used as a bioreductor in the green synthesis of AgNPs.

Process Optimization for Green Synthesis of Silver Nanoparticles Using Indonesian Medicinal Plant Extracts

“…Our previous study showed that among the three plant extracts, PN was the strongest antioxidant, followed by OS and then CL. This was indicated by their IC 50 value on the DPPH scavenging method, i.e., 102, 133, and 363 µg/mL, respectively [14]. In the biosynthesis of metal nanoparticles using plant extract, metal ions are reduced and then the reduced metal atoms undergo nucleation [23].…”

“…Our previous studies on seven native Indonesian plant species showed that the three plants commonly used in herbal medicines, namely Phyllanthus niruri (PN), Orthosiphon stamineus (OS), and Curcuma longa (CL), had the highest total phenolic and flavonoid contents and exhibited antioxidant activities [13,14]. These plants also demonstrated antibacterial activities against various pathogenic gram-positive and gram-negative bacteria [15][16][17][18][19][20].…”

Sosialisasi Jamu Saintifik Untuk Panyakit Diabetes Melitus

“…Penelitian praklinis dilakukan pada hewan percobaan di laboratorium farmakologi eksperimental. Bahan botani dipilih oleh Komisi Ilmiah Nasional untuk Pengobatan Herbal (Kartini et al, 2020). Bahan-bahan tersebut diproduksi berdasarkan bahan-bahan empiris yang telah ditinjau dalam literatur untuk kesesuaian penggunaan sosial.…”

Antioxidant Activity of Temulawak (Curcuma xanthorrhiza Roxb.) and its Classification with Chemometrics

“…Metode ini didasarkan pada pembentukan kompeks antara aluminium klorida dengan gugus keto pada atom C-4 dan gugus hidroksi pada atom C-3 atau C-5 yang bertetangga dari golongan flavon dan flavonol [20]. Beberapa penelitan yang menggunakan metode ini yaitu [20][21][22][23][24]. Nilai TFC dinyatakan sebagi mg ekuivalen rutin/g (mg RE/g) yaitu jumlah kesetaraan mg rutin dalam 1 g sampel.…”

“…Belum ditemukan penelitian terkait penentuan nilai TFC ekstrak metanol temulawak dengan metode sama untuk dibandingkan. Namun, beberapa penelitian terkait temulawak yang menentukan nilai TFC yaitu Sukweenadhi et al [25] untuk ekstrak etanol (34,62 ± 1,58 mg QE/100g) dan [24] untuk ekstrak air-etanol (2,77 ± 2,58 g QE/100g). QE adalah quercetin equivalent.…”