Selection of high temperature and salinity tolerant Trichoderma isolates with antagonistic activity against Sclerotium rolfsii

Abstract

Trichoderma isolates were collected from varied agro-climatic zones of India and screened for high temperature and salinity tolerance. Among all the isolates tested, T. asperellum, TaDOR673 was highly tolerant to heat shock of 52°C with a mean spore count (log c.f.u/ml) of 4.33. The isolate after recovery from heat shock possessed higher germination rate and biomass production compared to its wild counterpart, upon prolonged exposure to 37°C. Under stress, TaDOR673 accumulated >15% of trehalose and >5% of mannose and raffinose compared to the wild type strain signifying their role in stress tolerance. T. asperellum, TaDOR693 and T. asperellum, TaDORS3 were identified as superior salt-tolerant isolates. Interestingly, TaDOR673 also possessed similar tolerance levels to increasing saline concentrations as indicated by its improved colony growth under stress conditions. T. asperellum, TaDOR673 and T. asperellum, TaDOR7316 effectively controlled the collar rot disease in groundnut by 79.7% when screened in vitro and in vivo. Thus, the study identified a potential thermotolerant and saline tolerant strain of Trichoderma, TaDOR673 that could be used as potential bioagent in stressed soils.

Large‐scale Trichoderma diversity was associated with ecosystem, climate and geographic location

“…asperellum (belonging to the Viride Clade and more abundant in the coast ecosystem in this study, Fig. A) was able to tolerate salt stress at 1 M NaCl compared with about 0.6 M NaCl of seawater (Poosapati et al ., ), which is a possible reason for the enrichment of the Viride Clade in coastal ecosystems in the present study.…”

Short Communication: Viability and environmental effect to conidial germination of antagonistic fungi that potential as biological control of Colletotrichum gloeosporoides caused antracnose disease on chili

“…This indicates that Trichoderma was able to germinate above the optimum temperature. The results of research by Poosapati et al (2014) showed that the Trichoderma asperellum TaDOR673 fungi isolate was highly tolerant to 52C high temperature, and the fungi was still able to produce fairly dense conidial and higher conidial germination compared to other isolates.…”

“…Conidial germination of various antagonistic fungi is strongly influenced by changes in temperature and ultraviolet light exposure. According to Poosapati et al (2014) conidia of Trichoderma spp from different agro climatic zone of India reduced their ability to germinate at temperature 37°C except for T. asperellum-TaDOR673. This fungi isolate was highly tolerant at high temperature 52°C, and was still able to produce fairly dense conidial and higher conidial germination compared to other isolate.…”

Crop Vulnerability to Weather and Climate Risk: Analysis of Interacting Systems and Adaptation Efficacy for Sustainable Crop Production

“…Many Trichoderma species are capable of withstanding low and high temperatures. Two isolates of Trichoderma from soil samples collected from various regions of India withstood a heat shock of 52 • C and prolonged exposure to temperature of 37 • C while maintaining high tolerance to salinity [117]. Information from this study and others may be used to build a resilient tool that combines Trichoderma species based on their temperature and salinity response.…”