Selection, identification and application of a DNA aptamer against Listeria monocytogenes

Duan, Nuo; Ding, Xiaoxiao; He, Liangxing; Wu, Shijia; Wei, Yunxiao; Wang, Zhouping

doi:10.1016/j.foodcont.2013.03.011

Cited by 78 publications

(43 citation statements)

References 24 publications

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“…Aptamers are single-stranded nucleic acid molecules which derived from random single-stranded nucleic acid sequence pools and selected via the process called SELEX (Systematic Evolution of Ligands by Exponential Enrichment) (Ellington & Szostak, 1990;Robertson & Joyce, 1990;Tuerk & Gold, 1990). These selected aptamers can bind a wide range of targets, ranging from small molecules like ochratoxin A (Cruz-Aguado & Penner, 2008) to proteins (Cox & Ellington, 2001) as well as whole cells (Duan et al 2013) with high affinity and specificity. Owing to their high selectivity, stability, versatile target binding and easy regeneration capabilities over antibodies, aptamers have received considerable research attention as recognition probes in biodetections.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles

et al. 2015

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“…Whole cell SELEX is considered to be a useful tool for aptamer selection (Duan et al 2013;Moon et al 2015;Suh et al 2014). Since the entirety of the cell surface targets in their native three-dimensional conformations will be available in these SELEX processes (Dwivedi et al 2013;Shamah et al 2008), aptamers derived in this way would be a greater success in targeting pathogen cells than those generated from purified targets (Guo et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Identification and application of ssDNA aptamers against H37Rv in the detection of Mycobacterium tuberculosis

Aimaiti

Qin

Cao

et al. 2015

Appl Microbiol Biotechnol

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Microscopy of direct smear with the Ziehl-Neelsen stain is still broadly used in tuberculosis diagnosis. However, this method suffers from low specificity and is difficult to distinguish Mycobacterium tuberculosis (MTB) from nontuberculosis mycobacterial (NTM), since all mycobacterial species are positive in Ziehl-Neelsen stain. In this study, we utilized whole cell SELEX to obtain species-specific aptamers for increasing the specificity of MTB detection. Whole cell SELEX was performed in MTB reference strain H37Rv by two selection processes based on enzyme-linked plate or Eppendorf tube, respectively. To increase success rate of generating aptamers, the selection processes were systematically monitored to understand the dynamic evolution of aptamers against complex structure of target bacteria. Two preponderant groups and ten high-affinity aptamers were obtained by analyzing the dynamic evolution. Preponderant aptamer MA1 from group I showed relatively high binding affinity with apparent dissociation constant (KD value) of 12.02 nM. Sandwich ELISA assay revealed five aptamer combinations effectively bound MTB strains in preliminary evaluation, especially the combination based on aptamer MA2 (another preponderant aptamer from group II) and MA1. Further evaluated in many other strains, MA2/MA1 combination effectively identified MTB from NTM or other pathogenic bacteria, and displayed the high specificity and sensitivity. Binding analysis of aptamer MA1 or MA2 by fluorescence microscopy observation showed high binding reactivity with H37Rv, low apparent cross-reactivity with M. marinum, and no apparent cross-reactivity with Enterobacter cloacae. Taken together, this study provides attractive candidate species-specific aptamers to effectively capture or discriminate MTB strains.

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“…Moreover, we achieved the whole cell in vitro selection on L. monocytogenes and selected aptamer showed a considerable affinity (Kd = 48.74 ± 3.11 nM). A fluorescent binding bioassay was developed to confirm the high specific binding of these MREs towards targets demonstrated a LOD of 75 cfu/ mL (Duan et al 2013a). In summary, both bare and modified aptamers have significantly contributed in improved detection of pathogens and at the same time noticeably reduced the cost incurred for detection method(s) when compared to antibodies.…”

Section: Listeria Speciesmentioning

confidence: 98%

“…These oligonucleotides are able to form a specific hairpin loop allowing them to recognize a variety of targets even for closely related pathogenic bacteria (Table 1). Though all improvements, aptamer technology still needs refinements to obtain aptamers with target specificity and strong binding properties in different environmental Cell surface-associated proteins, teichoic acid recognition Abbaspour et al 2015;Baumstummler et al 2014;Chang et al 2013a;Moon et al 2015 Yersinia pestis Whole cell Duan et al 2016;Yuan et al 2014 Listeria monocytogenes ssDNA/ssDNA-digoxigenin ATCC pure culture Lipopolysaccharide, lipoprotein or outer membrane proteins Duan et al 2013a;Suh and Jaykus 2013 Pseudomonas aeroginosa FITC/QD-ssDNA Water 1-10 3 Whole-cell recognition Kim et al 2013a;Wang et al 2011;Wang et al 2012 Campylobacter jejuni FAM-ssDNA ssDNA-Biotin-qPCR Strains conditions like pH variation, nuclease presence, salt concentrations, and temperature Tibor Hianik 2007). Hence, this review aims to cover comprehensively current implemented bare and conjugated aptamer-based multiplex techniques in detection of food pathogens.…”

Section: Introductionmentioning

confidence: 99%

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

et al. 2017

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Foodborne ailments constitute a public health challenge and pose an incredible economic burden in healthcare system around the globe. This dilemma has urged authorities and other entities working in field of food quality control and supply chain to play a pivotal role in ensuring food safety. Analytical strategies have been developed using numerous systematic evolution of ligands by exponential enrichment (SELEX) methods to assure food safety. High-affinity and high-sensitivity ssDNA and RNA aptamers against pathogens have emerged as a novel strategy, as compared to the more resource-demanding and complicated biochemical test-based approaches. Thus, this review aims to focus on some methods used in the selection of specific bare, modified, and conjugated aptamers and on the further analysis of selected aptamers using flow cytometer or post-SELEX modifications for enhanced detection of frequently diagnosed foodborne bacteria such as Bacillus sp., Campylobacter jejuni, Escherichia sp., Salmonella sp., Staphylococcus aureus, Shigella sp., Listeria monocytogenes, and Streptococcus pyogenes and/or targeting their cell components towards attaining fast, sensitive, and selective methods for the detection of pathogens in food(s) or other sources.

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Selection, identification and application of a DNA aptamer against Listeria monocytogenes

Cited by 78 publications

References 24 publications

Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles

Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles

Identification and application of ssDNA aptamers against H37Rv in the detection of Mycobacterium tuberculosis

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

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