Selection for improved resistance to Aeromonas hydrophila in Indian major carp Labeo rohita: Survival and innate immune responses in first generation of resistant and susceptible lines

A linkage map of transcribed single nucleotide polymorphisms in rohu (Labeo rohita) and QTL associated with resistance to Aeromonas hydrophila

“…Mortalities occur quickly (sometimes within 30 hours after experimental challenge, [4]) and differences in the challenge infection procedure are believed to affect expression of the genetic potential to survive this disease. Even so, one generation of divergent selection based on challenge test data has been shown to result in significantly higher average rates of survival (73.3 ± 3.3% versus 16.7 ± 3.3%), blood phagocyte respiratory burst activity, serum myeloperoxidase activity and ceruloplasmin level in resistant compared to susceptible line rohu [5]. A major limitation to selective breeding is the inability to directly test highly valuable broodstock by challenging them to the disease.…”

Effects of dietary supplementation of potential probiotic Pseudomonas aeruginosa VSG-2 on the innate immunity and disease resistance of tropical freshwater fish, Labeo rohita

“…The global production of L. rohita was approximately 1.2 million tons in 2005, out of which nearly 1 million tons was contributed by India [2]. Bacterial infections are one of the most important causes of disease problems in Indian aquaculture [3]. Aeromonas hydrophila is the most common pathogen, and it can easily spread through accidental abrasions [4].…”

Analysis of immune-related ESTs and differential expression analysis of few important genes in lines of rohu (Labeo rohita) selected for resistance and susceptibility to Aeromonas hydrophila infection

“…First 15 families with higher survival record are considered as resistant line and last ten families having the lowest survival were considered as susceptible line. Offspring from both the lines were produced and challenged with same dose of A. hydrophila [14]. The second generations of resistant and susceptible lines were generated from first generation respective lines by taking 20 males and 20 females from each line.…”

“…Furthermore, limited sequence information was available for some immune-related genes such as complement component 3 (C3), G and C-type lysozymes, interleukin 1b (IL1b), b-2 microglobulin (b-2 M), CXCa, tumor necrosis factor a (TNFa), inducible nitric oxide synthase (iNOS), few tolllike receptors (TLRs), nucleotide binding and oligomerisation domain 2 (NOD 2) and transferrin, etc. [12][13][14][15] until the high throughput Illumina mRNA-seq carried out in our earlier study [16] from comparison of first generation selected resistant and susceptible lines of rohu raised against Aeromonas hydrophila infection. The methods used to generate these lines were described in Sahoo et al [14].…”

“…[12][13][14][15] until the high throughput Illumina mRNA-seq carried out in our earlier study [16] from comparison of first generation selected resistant and susceptible lines of rohu raised against Aeromonas hydrophila infection. The methods used to generate these lines were described in Sahoo et al [14]. Gene ontology terms broadly grouped 3,419 (7.7 %) contigs as involved in stress response, while 1,939 (4.4 %) were categorised as involved in immune system processes.…”