Analysis of immune-related ESTs and differential expression analysis of few important genes in lines of rohu (Labeo rohita) selected for resistance and susceptibility to Aeromonas hydrophila infection

Das, Satyajit; Chhottaray, Chiranjibi; Mahapatra, Kanta Das; Saha, Jatindra Nath; Baranski, Matthew; Robinson, Nick; Sahoo, P.K.

doi:10.1007/s11033-014-3625-4

Cited by 15 publications

(7 citation statements)

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“…Primer sequences of Hsp90, Hsp70, and Hsp30 were obtained from our previous study (Das et al 2014). β-actin was used as the reference gene (Robinson et al 2012).…”

Section: Quantitative Pcr (Qpcr)mentioning

confidence: 99%

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Das

Mohapatra

Sahoo

2015

Cell Stress and Chaperones

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“…Primer sequences of Hsp90, Hsp70, and Hsp30 were obtained from our previous study (Das et al 2014). β-actin was used as the reference gene (Robinson et al 2012).…”

Section: Quantitative Pcr (Qpcr)mentioning

confidence: 99%

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Das

Mohapatra

Sahoo

2015

Cell Stress and Chaperones

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“…The Indian major carp Labeo rohita (Hamilton), a cyprinid is a leading candidate species for freshwater aquaculture not only in India but also in the whole sub-continent of South-East Asia with the annual production of 1.5 million tonnes in 2012 [ 1 ]. Due to its immense economic, ecological and cultural importance it received lot of research interest in different areas including culture [ 2 ], breeding [ 3 ] immunology [ 4 ], disease ecology [ 5 ], reproductive physiology [ 6 ] and nutrition [ 7 ]. One of the major problem in this species is highly monsoon dependent breeding habit [ 8 ] and inability to breed in confined pond water without hormonal induction [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Although literatures on different aspects of reproduction and breeding are available [ 3 ], the genetic mechanism underlying gonad maturation and seasonal breeding in tropical climate has not been fully understood. Genetic studies have been performed in the past decade, which focused on selective breeding [ 15 ], development of genetic markers [ 16 ], development of linkage map [ 17 ] and collection of immune related genes [ 4 ]. Reproduction of fish is centrally controlled by brain, pituitary liver and gonad (BPGL axis) [ 18 ] and multiple genes are involved in this process.…”

Section: Introductionmentioning

confidence: 99%

Construction, De-Novo Assembly and Analysis of Transcriptome for Identification of Reproduction-Related Genes and Pathways from Rohu, Labeo rohita (Hamilton)

et al. 2015

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Rohu is a leading candidate species for freshwater aquaculture in South-East Asia. Unlike common carp the monsoon breeding habit of rohu restricts its seed production beyond season indicating strong genetic control over spawning. Genetic information is limited in this regard. The problem is exacerbated by the lack of genomic-resources. We identified 182 reproduction-related genes previously by Sanger-sequencing which were less to address the issue of seasonal spawning behaviour of this important carp. Therefore, the present work was taken up to generate transcriptome profile by mRNAseq. 16GB, 72bp paired end (PE) data was generated from the pooled-RNA of twelve-tissues from pre-spawning rohu using IlluminaGA-II-platform. There were 64.97 million high-quality reads producing 62,283 contigs and 88,612 numbers of transcripts using velvet and oases programs, respectively. Gene ontology annotation identified 940 reproduction-related genes consisting of 184 mainly associated with reproduction, 223 related to hormone-activity and receptor-binding, 178 receptor-activity and 355 embryonic-development related-proteins. The important reproduction-relevant pathways found in KEGG analysis were GnRH-signaling, oocyte-meiosis, steroid-biosynthesis, steroid-hormone biosynthesis, progesterone-mediated oocyte-maturation, retinol-metabolism, neuroactive-ligand-receptor interaction, neurotrophin-signaling and photo-transduction. Twenty nine simple sequence repeat containing sequences were also found out of which 12 repeat loci were polymorphic with mean expected-&-observed heterozygosity of 0.471 and 0.983 respectively. Quantitative RT-PCR analyses of 13-known and 6-unknown transcripts revealed differences in expression level between preparatory and post-spawning phase. These transcriptomic sequences have significantly increased the genetic-&-genomic resources for reproduction-research in Labeo rohita.

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“…Recently, a number of studies have generated abundant transcriptome data from blue catfish ( Ictalurus furcatus )19, blunt snout bream ( Megalobrama amblycephala )20, grass carp2122, and rohu ( Labeo rohita )23 after being infected with A. hydrophila . These studies have primarily focused on identifying immune-related genes and substantially improved our understanding of the host immune defense mechanisms against bacterial infection.…”

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confidence: 99%

A transcriptome analysis focusing on inflammation-related genes of grass carp intestines following infection with Aeromonas hydrophila

Song

Sun

et al. 2017

Sci Rep

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Inflammation is a protective response that is implicated in bacterial enteritis and other fish diseases.The inflammatory mechanisms behind Aeromonas hydrophila infections in fish remain poorly understood. In this study, we performed a de novo grass carp transcriptome assembly using Illumina's Solexa sequencing technique. On this basis we carried out a comparative analysis of intestinal transcriptomes from A. hydrophila-challenged and physiological saline solution (PSS/mock) -challenged fish, and 315 genes were up-regulated and 234 were down-regulated in the intestines infected with A. hydrophila. The GO enrichment analysis indicated that the differentially expressed genes were enriched to 12, 4, and 8 GO terms in biological process, molecular function, and cellular component, respectively. A KEGG analysis showed that 549 DEGs were involved in 165 pathways. Moreover, 15 DEGs were selected for quantitative real-time PCR analysis to validate the RNA-seq data. The results confirmed the consistency of the expression levels between RNA-seq and qPCR data. In addition, a time-course analysis of the mRNA expression of 12 inflammatory genes further demonstrated that the intestinal inflammatory responses to A. hydrophila infection simultaneously modulated gene expression variations. The present study provides intestine-specific transcriptome data, allowing us to unravel the mechanisms of intestinal inflammation triggered by bacterial pathogens.The grass carp (Ctenopharyngodon idella) is an intensively cultured and economically important herbivorous fish species in China. In grass carp aquaculture, the fish are often infected by bacterial, viral and parasitic pathogens. Aeromonas hydrophila, a Gram-negative aquatic bacterium, is regarded as the major bacterial pathogen causing intestinal inflammation in animal taxa, including farmed fish 1-4 . A. hydrophila-induced enteritis is probably the most widespread disease in grass carp, especially under intensive rearing conditions, which results in huge economic losses annually due to reduced growth and high mortality rates [5][6][7] . The molecular mechanisms and pathways that regulate bacterial-induced inflammatory processes in humans and other mammalian species are relatively well understood [8][9][10] . In fish, several inflammation-related genes, including chemokine (C-X-C motif) ligand 2 (CXCL2) 11 , CXCL10 12 , cyclooxygenase-2 (COX-2) 13 , interleukin-1β (IL-1β ) 4,13,14 , IL-8 15,16 , IL-17A/F2 17 , and tumor necrosis factor (TNF)-α 13,18 , have already been identified and characterized with regard to their involvement in inflammatory responses to bacterial infections. However, the definitive mechanisms responsible for the bacterial-induced intestinal inflammation in fish are still unclear. Therefore, further studies are required to identify more molecular components that mediate inflammatory reactions in fish.In the past few years, transcriptome sequencing technology has found broad applications in gene discovery and gene expression profiling. Recently, a number of studi...

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Analysis of immune-related ESTs and differential expression analysis of few important genes in lines of rohu (Labeo rohita) selected for resistance and susceptibility to Aeromonas hydrophila infection

Cited by 15 publications

References 55 publications

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Construction, De-Novo Assembly and Analysis of Transcriptome for Identification of Reproduction-Related Genes and Pathways from Rohu, Labeo rohita (Hamilton)

A transcriptome analysis focusing on inflammation-related genes of grass carp intestines following infection with Aeromonas hydrophila

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