Selection and proliferation of rapid growing cell lines from embryo derived cell cultures of yew tree (Taxus cuspidata Sieb. et Zucc)

Son, Sung Ho; Choi, Sung Mee; Choi, Kum Boo; Lee, Yun Hee; Lee, Dea Sook; Choi, Myung Suk; Park, Young Goo

doi:10.1007/bf02932380

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…Large explants generally survive better and grow more rapidly in comparison to relatively small explants at the initial stage of culture. High inoculum density of 20±30% (v/v) is required to assure minimal lag phase times and a significant growth of plant cells (Son et al, 1999a). The effect of inoculum density on biomass increase varies with the types of culture, plant species and culture period.…”

Section: Physical and Chemical Factors Affecting Cell Growthmentioning

confidence: 99%

“…The optimum bioreactor design for maximum growth and proliferation of different species must be evaluated systematically (Son et al, 1999a, b).…”

Section: Bioreactor Design For Micropropagationmentioning

confidence: 99%

“…Shoots grown in vitro derived from bud culture of Acanthopanox koreanum were used as explants to induce callus. Induced embryogenic determined cell (IEDC) lines were obtained with modified single cell cloning methods reported previously (Son et al, 1999a). Further selection of IEDC lines was followed by collection of high-density cells using the method of a sugar density gradient.…”

Section: Embryogenic Culture In Bioreactorsmentioning

confidence: 99%

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Application of bioreactors for large-scale micropropagation systems of plants

Paek¹,

Hahn²,

Son³

2001

In Vitro Cell.Dev.Biol.-Plant

125

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The application of bioreactor culture techniques for plant micropropagation is regarded as one of the ways to reduce production cost by scaling-up and automation. Recent experiments are restricted to a small number of species that, however, demonstrate the feasibility of this technology. Periodic immersion liquid culture using ebb and flood system and column-type bubble bioreactors equipped with a raft support system to maintain plant tissues at the air and liquid interface were found to be suitable for micropropagation of plants via the organogenic pathway. Balloon-type bubble bioreactors proved to be fit for micropropagation via somatic embryogenesis with less shear stress on cultured cells. Several cultivars of Lilium were successfully propagated using a two-stage culture method in one bioreactor. A large number of small-scale segments were cultured for 4 wk with periodic immersion liquid culture to induce multiple bulblets from each segment, then the bulblet induction medium was changed into bulblet growth medium by employing a submerged liquid bioreactor system. This culture method resulted in a nearly 10-fold increase in bulblet growth compared to conventional culture with solid medium. About 20 000 cuttings of virus-free potato could be obtained from 120 singlenode explants in a 20-liter balloon-type bubble bioreactor after 8 wk of culture. The percentage of ex vitro survival and root induction of the cuttings was more than 95%. Other successful results were obtained from the micropropagation and transplant production of chrysanthemum, sweetpotato, Chinese foxglove. Propagation systems via somatic embryogenesis in Acanthopanax koreanum and thornless Aralia elata were established using a liquid suspension of embryogenic determined cells. More than 500 000 somatic embryos in different stages were harvested from a 10-liter balloon-type bubble bioreactor after a 6-wk culture. Further development of these embryos in solid medium and eventually in the field was successful. The bioreactor system could reduce initial and operational cost for micropropagation, but further development of sophisticated technology might be needed to apply this system to plant micropropagation industries.

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Section: Physical and Chemical Factors Affecting Cell Growthmentioning

confidence: 99%

“…The optimum bioreactor design for maximum growth and proliferation of different species must be evaluated systematically (Son et al, 1999a, b).…”

Section: Bioreactor Design For Micropropagationmentioning

confidence: 99%

Section: Embryogenic Culture In Bioreactorsmentioning

confidence: 99%

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