Improving fed-batch culture efficiency of Rhodiola sachalinensis cells and optimizing flash extraction process of polysaccharides from the cultured cells by BBD–RSM

Hao, Yanling; Zhang, Kexin; Jin, Meizhong; Piao, Xiangfan; Lian, Mei-Lan; Jiang, Jun

doi:10.1016/j.indcrop.2023.116513

Cited by 10 publications

(5 citation statements)

References 51 publications

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“…A nearly circular contour line is found in Figure 6a, indicating a minor interaction between the ethanol concentration and extraction time; by contrast, oval contour lines are found in Figure 6b,c, indicating high interactions between the ethanol concentration and liquid−material ratio and the extraction time and liquid−material ratio [22], which is consistent with Table 4 The response surface plots also exhibited an interaction between both independent variables (Figure 6). A nearly circular contour line is found in Figure 6a, indicating a minor interaction between the ethanol concentration and extraction time; by contrast, oval contour lines are found in Figure 6b,c, indicating high interactions between the ethanol concentration and liquid−material ratio and the extraction time and liquid−material ratio [22], which is consistent with Table 4. The optimal ethanol concentration (63%), extraction time (69 s), and liquid−material ratio (57 mL/g) were obtained by applying the methodology of the desired function, and the predicted flavonoid yield was 7.12%.…”

Section: Flash Extraction Process Optimization Of Flavonoids From Ye-...supporting

confidence: 81%

Improving Flavonoid Accumulation of Bioreactor-Cultured Adventitious Roots in Oplopanax elatus Using Yeast Extract

Jin

Wang

et al. 2023

Plants

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Oplopanax elatus is an endangered medicinal plant, and adventitious root (AR) culture is an effective way to obtain its raw materials. Yeast extract (YE) is a lower-price elicitor and can efficiently promote metabolite synthesis. In this study, the bioreactor-cultured O. elatus ARs were treated with YE in a suspension culture system to investigate the elicitation effect of YE on flavonoid accumulation, serving for further industrial production. Among YE concentrations (25-250 mg/L), 100 mg/L YE was the most suitable for increasing the flavonoid accumulation. The ARs with various ages (35-, 40-, and 45-day-old) responded differently to YE stimulation, where the highest flavonoid accumulation was found when 35-day-old ARs were treated with 100 mg/L YE. After YE treatment, the flavonoid content increased, peaked at 4 days, and then decreased. By comparison, the flavonoid content and antioxidant activities in the YE group were obviously higher than those in the control. Subsequently, the flavonoids of ARs were extracted by flash extraction, where the optimized extraction process was: 63% ethanol, 69 s of extraction time, and a 57 mL/g liquid–material ratio. The findings provide a reference for the further industrial production of flavonoid-enriched O. elatus ARs, and the cultured ARs have potential application for the future production of products.

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Section: Flash Extraction Process Optimization Of Flavonoids From Ye-...supporting

confidence: 81%

Improving Flavonoid Accumulation of Bioreactor-Cultured Adventitious Roots in Oplopanax elatus Using Yeast Extract

Jin

Wang

et al. 2023

Plants

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“…According to statistics, the model's misfit was not significant (p>0.05). The trial's predicted and actual values were determined to be well-matched by the R 2 value of 0.9815. , indicating that this regression equation was reliable and could accurately reflect the relationship of phlorizin-Zn (Ⅱ) complex yield between response values and various experimental factors (Akalın et al, 2015;Cheng et al, 2020;Hao et al, 2023).…”

Section: Optimization Of the Preparation Process By Rsm Experimental ...mentioning

confidence: 77%

Optimization of Preparation Procedures of Phlorizin-Zn (Ⅱ) Complex and Its Antioxidant Activity Study in vitro

Zhang,

Li,

Yang

et al. 2023

American Journal of Biochemistry and Biotechnology

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Flavonoid metal complexes possess more effective antioxidant properties than flavonoids. However, the preparation of the phlorizin-Zn (Ⅱ) complex and its antioxidant activity has not been reported. In this research, the preparation procedures of the phlorizin-Zn (Ⅱ) complex were enhanced using by single factor test and Response Surface Methodology (RSM). The optimal preparation procedures of the phlorizin-Zn (II) complex were as follows: The time of 4.1 h, the pH of 5.9, the temperature of 48.7°C and the molar ratio (phlorizin: Zn 2+ ) of 1:1. Under these conditions, the predicted preparation yield of phlorizin-Zn (Ⅱ) complex was 79.052%. Additionally, the antioxidant activity of the phlorizin-Zn (Ⅱ) complex was analyzed in this research. 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH), 2, 2'-Azinobis (3-Ethylbenzothiazoline-6-Sulfonic acid) (ABTS), hydroxyl free radical and Ferric ion Reducing Antioxidant Power (FRAP) experiments revealed that phlorizin-Zn (Ⅱ) complex had significant DPPH, ABTS and hydroxyl free radical scavenging abilities and exhibited substantial reducing power in a dose-dependent manner. Moreover, the phlorizin-Zn (Ⅱ) complex displayed stronger in vitro antioxidant capacity compared with phlorizin. The findings demonstrated that the phlorizin-Zn (Ⅱ) complex had great potential in antioxidant applications in the future.

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“…The normal probability plot (NPP) of the residuals is a critical graphical approach for visualizing the residuals’ distribution and determining the model’s adequacy [ 72 ]. The residuals are the difference between the theoretical model’s predicted response values and the experimental response values.…”

Section: Resultsmentioning

confidence: 99%

Identification and statistical optimization of a novel alginate polymer extracted from newly isolated Synechocystis algini MNE ON864447 with antibacterial activity

Saad,

Sidkey,

El-Fakharany

2023

Microb Cell Fact

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Cyanobacteria are a potential source of promising secondary metabolites with different biological activities, including antibacterial, antiviral, antifungal, antiprotozoal, and anticancer activities. To combat the emergence of antibiotic resistance, there is an urgent requirement for new drugs, and cyanobacteria metabolites can constitute alternative new antibacterial medication. The chemical complexity of their exopolysaccharides indicates that they have the potential to be bioactive molecules with many biological activities. The present study aimed to produce and optimise a novel alginate polymer from a newly isolated cyanobacterium, S. algini MNE ON864447, in addition to its promising antibacterial activity. We successfully isolated a new cyanobacterium strain, S. algini MNE ON864447 from the Nile River, which produces alginate as an extracellular polymeric substance. The isolated cyanobacterial alginate was identified using a set of tests, including FTIR, TLC, HPLC, GC–MS, and 1H NMR. Plackett–Burman statistical design showed that working volume (X1), the incubation period (X2), and inoculum size (X3) are the most significant variables affecting the production of alginate. The highest alginate production (3.57 g/L) was obtained using 4% inoculum size in 400 mL medium/L conical flask after 20 days of the incubation period. The extracted alginate showed potent antibacterial activity against both Gram-negative and Gram-positive bacteria and Streptococcus mutants (NCTC10449) are the most sensitive tested pathogen for purified cyanobacterial alginate with inhibition zone diameters of 34 ± 0.1 mm at 10 mg/mL of purified alginate while Vibro cholera (NCTC 8021) the lowest sensitive one and showed inhibition zone diameters of 22.5 ± 0.05 mm at the same cyanobacterial alginate concentration. This antibacterial activity is a critical step in the development of antibacterial drugs and presents a new challenge to fight against multi-resistant bacteria.

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Improving fed-batch culture efficiency of Rhodiola sachalinensis cells and optimizing flash extraction process of polysaccharides from the cultured cells by BBD–RSM

Cited by 10 publications

References 51 publications

Improving Flavonoid Accumulation of Bioreactor-Cultured Adventitious Roots in Oplopanax elatus Using Yeast Extract

Improving Flavonoid Accumulation of Bioreactor-Cultured Adventitious Roots in Oplopanax elatus Using Yeast Extract

Optimization of Preparation Procedures of Phlorizin-Zn (Ⅱ) Complex and Its Antioxidant Activity Study in vitro

Identification and statistical optimization of a novel alginate polymer extracted from newly isolated Synechocystis algini MNE ON864447 with antibacterial activity

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