Seleção deprimerspara análise deinter simple sequence repeatsna cultivar ‘Itália’ deVitis viníferaL.

Pepineli, Afonso Carrasco; Strioto, Danuza Kelly; Marinelli, Giovana Carniatto; Mangolin, Claudete Aparecida; Machado, Maria de Fátima Pires da Silva

doi:10.1051/ctv/20142902081

Cited by 2 publications

(1 citation statement)

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“…Advancing the genetic breeding programs of the species may be achieved using molecular markers. Our evaluation of nanche accessions of the Embrapa Eastern Amazon BAG using ISSR markers revealed the need to identify specific temperatures for each of the primers used, as has previously been observed in other species (Pessoni, 2007;Souza et al, 2013;Pepineli et al, 2014). In a similar study conducted for Platonia insignis, 72 genotypes from the Embrapa Mid-North germplasm collection were evaluated using 18 primers.…”

Section: Discussionmentioning

confidence: 83%

Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers

Rodrigues¹,

Moura²,

Ramos³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Native of the Amazon, the nanche (Byrsonima crassifolia) is a fruit cultivated by family farmers and used in cooking; as such, it represents an opportunity for regional agribusiness. The Embrapa Eastern Amazon set up an active germplasm bank (BAG) consisting of 22 accessions sampled in 11 municipalities of Pará State. Due to its economic potential, there is an interest to advance the genetic breeding program of this species. The aim of this study was to characterize the BAG nanche collection using inter-simple sequence repeat (ISSR) markers. Accessions were genotyped using 23 preselected ISSR primers resulting in 109 amplified polymorphic and 51 monomorphic bands. With eight polymorphic bands each, the most polymorphic primers were UBC 809 and UBC 848. An unweighted pair-group method with arithmetic average cluster analysis based on Jaccard's coefficient indicated that the individuals clustered into two distinct groups. Accessions Igarapé Açu-2 and Augusto Corrêa-Pl 1 were most similar. The genetic dissimilarity values ranged from 0.10 to 0.59. We conclude that the ISSR markers were efficient in detecting polymorphisms in the nanche accessions, and that it is possible to infer the genetic variability among accessions of the collection. This demonstrate the importance of using molecular markers in poorly studied species and the advantages that this information can bring to the genetic improvement of such species.

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Section: Discussionmentioning

confidence: 83%