Clonal propagation of cv. Italy grapes and the generation of genetic divergence among vineyards

Strioto, Danuza Kelly; Pepineli, Afonso Carrasco; Eisele, Tauana Gibim; Marinelli, Giovana Carniatto; Mangolin, Claudete Aparecida; Cantagali, Liriana Belizário; Oliveira-Collet, Sandra Aparecida de; Machado, Maria de Fátima Pires da Silva

doi:10.1016/j.scienta.2018.09.049

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…In the present study, the majority of the clones within a variety revealed no difference and were grouped as one variety cluster when all 492 individuals were subjected to neighbor-joining cluster analysis (cluster not provided). However, some of them such as Sev Areni, Nazeli, Garan Dmak, and Mskhali underwent somatic mutations and showed varying genotypes and, to some extent, varying phenotypes analogous [ 56 , 57 ]. Observed variations of SSR length can occur naturally during vine growth mainly due to diverse types of mutations, which are responsible for intravarietal diversity.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Armenian Grapevine (Vitis vinifera L.) Germplasm: Molecular Characterization and Parentage Analysis

Margaryan

Melyan

Röckel

et al. 2021

Biology

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Armenia is an important country of origin of cultivated Vitis vinifera subsp. vinifera and wild Vitis vinifera subsp. sylvestris and has played a key role in the long history of grape cultivation in the Southern Caucasus. The existence of immense grapevine biodiversity in a small territory is strongly linked with unique relief and diverse climate conditions assembled with millennium-lasting cultural and historical context. In the present in-depth study using 25 nSSR markers, 492 samples collected in old vineyards, home gardens, and private collections were genotyped. For verification of cultivar identity, the symbiotic approach combining genotypic and phenotypic characterization for each genotype was carried out. The study provided 221 unique varieties, including 5 mutants, from which 66 were widely grown, neglected or minor autochthonous grapevine varieties, 49 turned out to be new bred cultivars created within the national breeding programs mainly during Soviet Era and 34 were non-Armenian varieties with different countries of origin. No references and corresponding genetic profiles existed for 67 genotypes. Parentage analysis was performed inferring 62 trios with 53 out of them having not been previously reported and 185 half-kinships. Instability of grapevine cultivars was detected, showing allelic variants, with three and in rare cases four alleles at one loci. Obtained results have great importance and revealed that Armenia conserved an extensive grape genetic diversity despite geographical isolation and low material exchange. This gene pool richness represents a huge reservoir of under-explored genetic diversity.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Armenian Grapevine (Vitis vinifera L.) Germplasm: Molecular Characterization and Parentage Analysis

Margaryan

Melyan

Röckel

et al. 2021

Biology

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“…These intra-varietal special characteristics can be minor changes in morphological traits, such as bunch compactness and canopy thickness, or macroscopic modifications, such as fruit color (e.g., the grey and white variants of Pinot noir) [4][5][6][7]. It is known that such polymorphisms derive from somatic mutations that occur accidentally after reproduction by vegetative propagation [1,8,9]. Moreover, it has been recently ascertained that intra-varietal differences can arise as plant responses to the environment through epigenetic modifications (clone-dependent DNA methylation patterns) that affect gene expression without altering the DNA sequence [10,11].…”

Section: Introductionmentioning

confidence: 99%

Study of Inter- and Intra-varietal Genetic Variability in Grapevine Cultivars

Zombardo

Meneghetti²,

Morreale

et al. 2022

Plants

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Vitis vinifera includes a large number of cultivars that are further distinguished in biotypes and clones, and it is actually hard to differentiate them, even through complex molecular techniques. In this work, the plant materials of 56 putative Sangiovese and 14 putative Montepulciano biotypes, two of the most widespread black-berried Italian cultivars, were collected in different wine-growing areas of Italy distributed in 13 regions, from north to south. Firstly, the samples were analyzed using SSR markers to have proper varietal identification. According to the results, the genotypes belonged to three different cultivars: Sangiovese, Sanforte, and Montepulciano. Subsequently, the samples were investigated using AFLP, SAMPL, M-AFLP, and I-SSR molecular markers to estimate their intra-varietal genetic variability. The DNA marker-based method used turned out to be performing to bring out the geographic differences among the biotypes screened, and it can therefore be considered as a powerful tool available for all the grapevine varieties.

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“…Italia is smaller than the polymorphism detected in microsatellite loci (11.8%) of the cv. Italia vineyards distributed in different regions of the states of São Paulo and Paraná (Maia et al ., 2009), and also smaller than the polymorphism of ISSR markers (12%) (Strioto et al ., 2019). However, IRAP and REMAP retrotransposons may be a promising strategy to analyse DNA-level polymorphism of samples from the five cultivars because 349 sequences have been generated that may be used to compare the samples.…”

Section: Discussionmentioning

confidence: 99%

Development and use of retrotransposons-based markers (IRAP/REMAP) to assess genetic divergence among table grape cultivars

Strioto

Kuhn

Nagata

et al. 2019

Plant Genet. Resour.

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For more than four decades after the introduction of cv. Italia (Vitis vinifera L.) in Brazil, several somatic mutations in the genome of cv. Italia and its somatic mutants gave rise to phenotypes which generated at least five new cultivars of fine table grapes. Since no molecular marker proved to be effective in discriminating cv. Italia (V. vinifera L.) and its coloured mutants (Rubi, Benitaka, Brasil, Black Star), primers for the long terminal repeat (LTR) sequences were developed to analyse Inter Retrotransposon Amplified Polymorphism (IRAP) and Retrotransposon-Microsatellite Amplified Polymorphism (REMAP), and investigate how the coloured cultivars derived from clonal propagations of somatic mutations are genetically structured. Primers for LTR sequences of IRAP and REMAP markers were edited from grape sequence databases available at a GenBank. Twenty-four primers, denominated DKS001–DKS024, were edited. Three hundred and forty-nine DNA segments were amplified by individual DKS primers and DKS/ISSR (Inter Simple Sequence Repeats) primer combinations, at an average of 13.96 amplicons per primer pair. High genetic divergence between the five cultivars was inferred from polymorphism in retrotransposons IRAP and REMAP. The analysis of polymorphism of IRAP and REMAP retrotransposons was crucial to show that clonal propagation of somatic mutations may lead towards the formation of genetically divergent cultivars by the formation of genetically structured vineyards and show the mixture of genomes within each cultivar.

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Clonal propagation of cv. Italy grapes and the generation of genetic divergence among vineyards

Cited by 5 publications

References 38 publications

Genetic Diversity of Armenian Grapevine (Vitis vinifera L.) Germplasm: Molecular Characterization and Parentage Analysis

Genetic Diversity of Armenian Grapevine (Vitis vinifera L.) Germplasm: Molecular Characterization and Parentage Analysis

Study of Inter- and Intra-varietal Genetic Variability in Grapevine Cultivars

Development and use of retrotransposons-based markers (IRAP/REMAP) to assess genetic divergence among table grape cultivars

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