Seleção assistida com uso de marcador molecular para resistência a potyvírus em pimentão

Abstract

Resumo -O objetivo deste trabalho foi determinar a presença do alelo Pvr4, que confere resistência contra o PepYMV (Pepper yellow mosaic virus), em genótipos de pimentão comunmente encontrados no mercado brasileiro, com uso de um marcador molecular codominante tipo CAPS. A resistência ao PepYMV, nos genótipos CM-334-INRA, Myr-29 e em genótipos derivados do híbrido comercial Mônica-R, foi detectada como associada à banda de 444 pb, ligada ao alelo de resistência Pvr4. As plantas resistentes homozigotas (Pvr4/Pvr4) mostraram uma banda de 444 pb, as suscetíveis (Pvr4 Marker-assisted selection for resistance to potyvirus in sweet pepperAbstract -The objective of this work was to determine the presence of the Pvr4 allele, which controls the resistance to the PepYMV (Pepper yellow mosaic virus), in sweet pepper genotypes commonly available in the Brazilian market, using a CAPS codominant molecular marker. The resistance to PepYMV, in the genotypes CM-334-INRA, Myr-29, and in genotypes derived from the hybrid Mônica-R, was found to be associated with the 444 bp band linked to the resistance allele Pvr4. Homozygous resistant plants (Pvr4/Pvr4) showed a single band of 444 bp, the susceptible ones (Pvr4showed a band of 458 bp, and the heterozygous resistant plants (Pvr4 + /Pvr4) showed both bands. However, in the resistant accession CM-334-UFV, and in the hybrids Magali-R and Martha-R, as well as in populations derived from this accession and these hybrids, the resistance to PepYMV was not associated to the CAPS marker. The accession CM-334-UFV ('Criollo de Morelos-334' from Viçosa, MG, Brazil) was distinct from CM-334-INRA ('Criollo de Morelos-334', from France); although both accessions were resistant to PepYMV, the association of resistance with the 444 bp band was found only in CM-334-INRA.

Research Article Multiple genetic resistances in Capsicum spp.

“…For resistance to anthracnose, we only used the susceptible cultivar, 'Ikeda', as a control since there are no C. gloeosporioides resistant genotypes (Diao et al, 2017). For resistance to PepYMV, Criollo de Morelos 334 (CM-334) was used as a resistance standard (Nogueira et al, 2012), and 'Ikeda' as a susceptible standard.…”

“…Seven genic loci have already been identified in pvr series imparting resistance to the Potyvirus genus in Capsicum plants. Among them,five are recessive (pvr1,pvr2,pvr3,pvr5,and pvr6) and two are dominant (Pvr4 and Pvr7) (Parrela et al, 2002;Nogueira et al, 2012). Identifying and manipulating these or other new resistance genes have great importance for the development of new resistant cultivars.…”

A new resistance gene to PepYMV (Pepper yellow mosaic virus) in Capsicum annuum L.

“…The main resistance sources to potyviruses carry one of the genes from pvr series, denominated as pvr1 , pvr2 , pv r3 , Pvr4 , pvr5 , pvr6 and Pvr7 (Caranta, Thabuis, & Palloix, ; Grube, Blauth, Arnedo Andrés, Caranta, & Jahn, ; Parrella et al., ). Most of these genes are effective against two or more species of potyvirus (Nogueira et al., ). From the pvr series described by Grube, Blauth et al.…”

“…() , only Pvr4 and Pvr7 have monogenic dominant inheritance. These two genes are located on chromosome 10 of Capsicum annuum , approximately 2 cM apart (Grube, Blauth, et al., ; Nogueira et al., ). Recently, Venkatesh, An, Kang, Jahn, and Kang () through sequencing analysis using SNPs delimited the Pvr7 gene region at a 258Kb interval, and Pvr4 was found within the same region, therefore they concluded that Pvr4 and Pvr7 should be considered the same gene.…”

“…This marker is 2.1 ± 0.8 cM of the Pvr4 gene and generates a 458 bp amplicon in the susceptible and a 444 bp amplicon in the resistant genotypes. The resistant genotype CM‐334 used in this identification was from INRA ( Institut National de la Recherche Agronomique ) (CM‐334‐INRA) (Nogueira et al., ).…”