Research Article Multiple genetic resistances in Capsicum spp.

Bento, Cíntia dos Santos; Souza, A G de; Sudré, Cláudia Pombo; Pimenta, Samy; Rodrigues, Rosana

doi:10.4238/gmr16039789

Cited by 28 publications

(12 citation statements)

References 46 publications

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“…UENF 1381 is a Capsicum annuum var annuum accession from the UENF genebank, it is pungent and identified as resistant to bacterial spot [9] and anthracnose. ‘Ikeda’ is a very traditional sweet pepper ( Capsicum annuum ) genotype that has been used by small farmers for a long time, and it is susceptible to bacterial spot and also to anthracnose [10].…”

Section: Methodsmentioning

confidence: 99%

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

et al. 2019

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There are several phytosanitary problems that have been causing serious damage to the Capsicum crops, including anthracnose. Upon attack by certain pathogens, various protein molecules are produced, which are known as proteins related to pathogenesis (PR proteins), including antimicrobial peptides such as protease inhibitors, defensins and lipid transfer proteins (LTPs). The objective of this work is to identify antimicrobial proteins and/or peptides of two genotypes from Capsicum annuum fruits infected with Colletotrichum gloeosporioides. The fungus was inoculated into Capsicum fruits by the deposition of a spore suspension (106 conidia ml−1), and after 24 and 48 h intervals, the fruits were removed from the humid chamber and subjected to a protein extraction process. Protein analysis of the extracts was performed by tricine gel electrophoresis and Western blotting. The distinctive bands between genotypes in the electrophoresis profiles were subjected to mass spectrometry sequencing. Trypsin inhibition assays, reverse zymographic detection of protease inhibition and β-1,3-glucanase activity assays were also performed and extracts were also tested for their ability to inhibit the growth of C. gloeosporioides fungi ‘in vitro’. There were several low molecular weight proteins in all treated samples, and some treatments in which antimicrobial peptides such as defensin, lipid transfer protein (LTP) and protease inhibitor have been identified. It was shown that the green fruits are more responsive to infection, showing the production of antimicrobial peptides in response to injury and inoculation of the fungus, what did not occur in ripe fruits under any treatment.

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Section: Methodsmentioning

confidence: 99%

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

et al. 2019

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“…Previous studies identifying sources of resistance in Capsicum species in response to Colletotrichum spp. infection showed that ripe fruits are more resistant to anthracnose compared to unripe www.nature.com/scientificreports/ fruits 17,[40][41][42] . This could be explained by the rapidly induced expression of CaEAS and CaEAH genes in response to C. scovillei in ripe pepper fruits, while in unripe fruits a late and high upregulation of capsidiol biosynthetic genes were observed.…”

Section: Discussionmentioning

confidence: 99%

“…Seeds of Capsicum annuum accession from GBUEL103 (susceptible) and GBUEL104 (resistant to bacterial spot, pepper yellow mosaic virus, and anthracnose 41 ) were obtained from the Universidade Estadual de Londrina (UEL) seed germplasm. Samples were sown on a tray with organic plant substrates and, after the emergence of two pairs of true leaves, seedlings were transferred individually to plastic pots containing a mixture of soil and substrate (2:1, w:w ratio).…”

Section: Methodsmentioning

confidence: 99%

Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages

Baba

Powell

Ivamoto

et al. 2020

Sci Rep

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Capsicum annuum is one of the most important horticultural crops worldwide. Anthracnose disease (Colletotrichum spp.) is a major constraint for chili production, causing substantial losses. Capsidiol is a sesquiterpene phytoalexin present in pepper fruits that can enhance plant resistance. The genetic mechanisms involved in capisidiol biosynthesis are still poorly understood. In this study, a 3′ RNA sequencing approach was used to develop the transcriptional profile dataset of C. annuum genes in unripe (Uf) and ripe fruits (Rf) in response to C. scovillei infection. Results showed 4,845 upregulated and 4,720 downregulated genes in UF, and 2,560 upregulated and 1,762 downregulated genes in RF under fungus inoculation. Four capsidiol-related genes were selected for RT-qPCR analysis, two 5-epiaristolochene synthase (CA12g05030, CA02g09520) and two 5-epi-aristolochene-1,3-dihydroxylase genes (CA12g05070, CA01g05990). CA12g05030 and CA01g05990 genes showed an early response to fungus infection in RF (24 h post-inoculation-HPI), being 68-fold and 53-fold more expressed at 96 HPI, respectively. In UF, all genes showed a late response, especially CA12g05030, which was 700-fold more expressed at 96 HPI compared to control plants. We are proving here the first high-throughput expression dataset of pepper fruits in response to anthracnose disease in order to contribute for future pepper breeding programs. Chili peppers originate from the Americas and they are among the oldest cultivated plants, dating from before 3400 BC. Capsicum annuum L. is the most commercially important species, due to its usefulness in the human diet, medicines, beverages, and as ornamentals. Pepper fruits have several nutraceutical benefits for human health, due to a variety of antioxidant, anti-inflammatory, antimicrobial, anti-carcinogenic, and cardio-protective properties 1. Anthracnose is caused by Colletotrichum spp., and represents the major disease of chili fruit worldwide, leading to significant postharvest yield loss and reducing marketability 2. Twenty-four Colletotrichum species have been identified as pathogens of chili anthracnose, with the three main pathogenic species being C. scovillei (previously identified as C. acutatum), C. truncatum (syn. C. capsici), and C. siamense (previously identified as C. gloeosporioides) 3 .

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“…against to anthracnose caused by Colletotrichum spp. (Parey et al, 2013;Begum et al, 2015;Arunakumara and Satyanarayana, 2016;Bento et al, 2017;Maracahipes et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Screening of chilli pepper genotypes against anthracnose (Colletotrichum brevisporum)

Souza¹,

Assis²,

Catarino³

et al. 2020

Emir J Food Agric

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Anthracnose is the most important disease of chili pepper pimenta-de-cheiro (Capsicum chinense Jacq.). This species is widely cultivated in dryland areas in the Amazon, presenting high genetic diversity. Therefore, it presents a high potential for use in breeding. The objective of this study was to select pepper genotypes with potential resistance to anthracnose (Colletotrichum brevisporum). For this purpose, ripe fruits of pepper were acquired from the producing farms in the municipalities of Iranduba, Manacapuru, Rio Preto da Eva and Presidente Figueiredo. The experiments were conducted in seedlings (Phase I) and in mature and immature fruits (Phase II). The evaluation of the disease severity was performed using a scale of scores. The morphoagronomic characterization of the genotypes considered the fruits in the immature and mature stages and plant growth habit. The injured areas in the immature fruits ranged from 0.3 to 9.7 cm2 and in the mature areas, it ranged from 0.2 to 9.9 cm2. The genotypes RPE41 and MPU29 indicated resistance to anthracnose in both stages. The morphoagronomic characterization of the fruits revealed variability for mass (5.58 to 13.74 g), length [C] (4.08 to 8.16 cm), diameter [D] (1.74 to 2.54 cm), L/D ratio (1.88 to 4.70) and color of the fruit.

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Research Article Multiple genetic resistances in Capsicum spp.

Cited by 28 publications

References 46 publications

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages

Screening of chilli pepper genotypes against anthracnose (Colletotrichum brevisporum)

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