Seed oil determination by pulsed nuclear magnetic resonance without weighing and drying seeds

Tiwari, P. N.; Burk, Wolfgang

doi:10.1007/bf02678819

Cited by 31 publications

(10 citation statements)

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“…The N, P and K uptake in plant samples was worked out by multiplying their content with the corresponding yield. The oil content in mustard seed was estimated by Pulse Nuclear Magnetic Resonance (NMR) Technique (Tiwari and Burk, 1980). Economic analysis of data was done based on the prevailing cost of inputs/operations and price of produce.…”

Section: Methodsmentioning

confidence: 99%

Productivity, Nutrient Uptake and Profitability of Sweet Sorghum -Mustard Cropping System under Different Levels of Nitrogen

Miri¹,

Rana²

2014

AJAST

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A field experiment was conducted during summer and winter seasons of 2009-10 and 2010-11 at Research Farm of Division of Agronomy, Indian Agricultural Research Institute, New Delhi to study the effects of nitrogen levels applied to sweet sorghum genotypes on productivity, nutrient uptake and profitability of sweet sorghum-mustard cropping system. Pooled analysis of data indicated that there was significant increase in green biomass, stalk, juice, brix (%), fermentable sugar and estimated ethanol yield due to application of nitrogen up to 100 kg N ha -1 over control. The highest grain yield (2.7 t ha -1 ) and fodder yield (15.6 t ha -1 ) were recorded with application of 150 kg N ha -1 . Pooled analysis of data showed that hybrid 'CSH 22 SS' recorded the highest stalks (85.6 t ha -1 ), juice (30 t ha -1 ) , fermentable sugar (4.6 t ha -1 ) and estimated ethanol yield ( 2.6 kl ha -1 ). On an average grain yield (2.5 t ha -1 ) of hybrid 'CSH 22 SS' was 31.5% and 45.8% higher than 'RSSV 9' and 'SSV 84', respectively. Application of 150 kg N ha -1 to preceding sweet sorghum produced the highest values of plant height, number of branches plant -1 , number of siliqua plant -1 , number of seeds siliqua -1 , siliqua length, seed yield, biological yield and oil yield. Oil percent in mustard declined as nitrogen dose applied to preceding sweet sorghum increased. Residual effect of sweet sorghum genotypes on performance of succeeding mustard was not significant. Productivity, nutrient uptake and economics of sweet sorghum-mustard cropping system significantly increased due to application of nitrogen up to 150 kg ha -1 . The highest sorghum equivalent yield (6.2 t ha -1 ), net returns ( 66300= US $ 1326) and net benefit cost ratio (2.8) were recorded when 150 kg N ha -1 was applied. Among genotypes, including of CSH 22 SS hybrid significantly enhanced productivity, nutrient uptake and economics of system.

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Section: Methodsmentioning

confidence: 99%

Productivity, Nutrient Uptake and Profitability of Sweet Sorghum -Mustard Cropping System under Different Levels of Nitrogen

Miri¹,

Rana²

2014

AJAST

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“…d Determined by means of NMR (nuclear magnetic resonance) on the basis of 6% moisture (Tiwari & Burk, 1980) . e Determined by the Palladium test (Thies, 1989).…”

Section: Variation Within CC Hispanicamentioning

confidence: 99%

Variation for agronomic characteristics in Crambe hispanica, a wild relative of Crambe abyssinica

Jc¹,

Mastebroek²

1996

Euphytica

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In order to assess the potential of Crambe hispanica, either in breeding programmes of C . abyssinica or as an oilseed crop in itself, 36 accessions of C. hispanica (29 of var. hispanica and 7 of var. glabrata) were grown in a greenhouse and evaluated for morphological characteristics, earliness, plant habit, seed characteristics and fatty acid composition . Four lines of C. abyssinica were included for reference . The 29 accessions of var . hispanica showed significant variation for all observed characteristics . Besides morphological characteristics, large variation was found for earliness, number of primary branches, seed yield, 1000 seed weight and volume, and linoleic, linolenic and erucic acid content . Morphological characteristics, earliness and plant habit did not show any high correlations with seed characteristics or fatty acid composition, except for seed hull (pericarp) mottling, which was related to a high oil and erucic acid content . The seven accessions of var. glabrata showed little variation . The large genetic variation in combination with promising figures for several characteristics, found in C . hispanica, may be useful in breeding programmes of the oilseed crop C . abyssinica, for which the available genetic variation is limited : Prospects of selection for high-erucic acid genotypes are discussed . Compared to C. abyssinica, both botanical varieties of C. hispanica are characterized by a cordate shape of the basal leaves, lack of seed retention and a lower DNA content. Plants of var. glabrata differed from var. hispanica in a sparsely hispid upper leaf surface and round stems and branches covered with a waxy layer . These clear differences and lack of success in intercrossing both varieties of C. hispanica strongly suggest that their taxonomic classification should be reconsidered .

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“…The Hahn spin-echo signal has no information that can be used to predict seed protein content directly or indirectly from oil measurements (recent literature has shown there is at best a weak negative correlation between protein and oil in cottonseed [12,13]). Other studies have used the FID, FID-CPMG or CPMG signal regions to develop prediction models to measure seed reserve content [7][8][9]. Initially we attempted to use CPMG-based models for seed protein and oil quantification using a reference set of values from transgenic cottonseed lines (BnFAD2) [27] that covered a broad range of protein and oil values.…”

Section: Modified Combined Relaxation Protein and Oil Quantificationmentioning

confidence: 99%

“…NMR is based on the absorption of energy by an atomic nucleus changing its spin orientation in a magnetic field [6]. Manipulation of all the hydrogen nuclei within the entire bulk of the oilseed samples using radiofrequency pulse sequences [time-domain nuclear magnetic resonance (TD-NMR), also known as low-field NMR] produces characteristic relaxation signals for protein, carbohydrate, moisture, and oil [7]. This technique is not affected by the color or opacity of the samples and thus provides true bulk estimation of compositional information even for large samples.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Quantification of Oil and Protein in Cottonseed by Low‐Field Time‐Domain Nuclear Magnetic Resonance

Horn

Neogi

Tombokan

et al. 2011

J Americ Oil Chem Soc

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Modification of cottonseed quality traits is likely to be achieved through a combination of genetic modification, manipulation of nutrient allocation, and selective breeding. Oil and protein stores account for the majority of mass of cottonseed embryos. A more comprehensive understanding of the relationship between lint quality, lint yield, and embryo reserve accumulation will assist breeders in their efforts to improve seed value. Here we report the development of a rapid, nondestructive, simultaneous method for quantifying oil and protein levels within cottonseed by low-field 1 H time-domain nuclear magnetic resonance (TD-NMR). This approach is suitable for a minimal amount of seed and represents an accurate (oil R 2 = 0.998, protein R 2 = 0.95), noninvasive alternative to conventional, time-consuming chemical extractions. We demonstrate the value of this approach by surveying seed reserve content, identifying extremes of either protein and/or oil, in two sets of diverse germplasm.

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Seed oil determination by pulsed nuclear magnetic resonance without weighing and drying seeds

Cited by 31 publications

References 1 publication

Productivity, Nutrient Uptake and Profitability of Sweet Sorghum -Mustard Cropping System under Different Levels of Nitrogen

Productivity, Nutrient Uptake and Profitability of Sweet Sorghum -Mustard Cropping System under Different Levels of Nitrogen

Variation for agronomic characteristics in Crambe hispanica, a wild relative of Crambe abyssinica

Simultaneous Quantification of Oil and Protein in Cottonseed by Low‐Field Time‐Domain Nuclear Magnetic Resonance

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