Simultaneous Quantification of Oil and Protein in Cottonseed by Low‐Field Time‐Domain Nuclear Magnetic Resonance

Horn, Patrick J.; Neogi, Purnima B.; Tombokan, Xenia; Ghosh, S. K.; Campbell, B. Todd; Chapman, Kent D.

doi:10.1007/s11746-011-1829-5

Cited by 39 publications

(37 citation statements)

References 18 publications

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“…Cotton is a typical oilseed, storing lipids and proteins in cytosolic organelles of mature seed tissues. In cottonseeds, the amount of stored lipid is ;20% of the seed dry weight (Horn et al, 2011b), making refined cottonseed oil a valuable coproduct of fiber production. However, the antinutritional secondary metabolite, gossypol, must be removed from seed oil extracts through the refining process, and gossypol residues in the protein meal limit its use as a high-value animal feed (Jones and King, 1996).…”

Section: Introductionmentioning

confidence: 99%

Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

et al. 2012

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Advances in mass spectrometry (MS) have made comprehensive lipidomics analysis of complex tissues relatively commonplace. These compositional analyses, although able to resolve hundreds of molecular species of lipids in single extracts, lose the original cellular context from which these lipids are derived. Recently, high-resolution MS of individual lipid droplets from seed tissues indicated organelle-to-organelle variation in lipid composition, suggesting that heterogeneity of lipid distributions at the cellular level may be prevalent. Here, we employed matrix-assisted laser desorption/ ionization-MS imaging (MALDI-MSI) approaches to visualize lipid species directly in seed tissues of upland cotton (Gossypium hirsutum). MS imaging of cryosections of mature cotton embryos revealed a distinct, heterogeneous distribution of molecular species of triacylglycerols and phosphatidylcholines, the major storage and membrane lipid classes in cotton embryos. Other lipids were imaged, including phosphatidylethanolamines, phosphatidic acids, sterols, and gossypol, indicating the broad range of metabolites and applications for this chemical visualization approach. We conclude that comprehensive lipidomics images generated by MALDI-MSI report accurate, relative amounts of lipid species in plant tissues and reveal previously unseen differences in spatial distributions providing for a new level of understanding in cellular biochemistry.

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Section: Introductionmentioning

confidence: 99%

Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

et al. 2012

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“…The use of high-field NMR to screen seeds and grains based on their quality characteristics, that is, protein content and quality, is not always feasible due to complicated procedures required for sample preparation as well as the cost of the required instruments (Gouilleux, Marchand, Charrier, Remaud, & Giraudeau, 2018;Marcone et al, 2013). This technique has been mostly used for quality characterization and screening of oilseeds (Horn et al, 2011;Melchinger et al, 2017Melchinger et al, , 2018Pedersen, Munck, & Engelsen, 2000), although a few studies have been conducted to examine the possibility of using LF-NMR for cereal screening (Brusewitz & Stone, 1987;Rolletschek et al, 2015). This technique has been mostly used for quality characterization and screening of oilseeds (Horn et al, 2011;Melchinger et al, 2017Melchinger et al, , 2018Pedersen, Munck, & Engelsen, 2000), although a few studies have been conducted to examine the possibility of using LF-NMR for cereal screening (Brusewitz & Stone, 1987;Rolletschek et al, 2015).…”

Section: Resonance Spectroscopymentioning

confidence: 99%

“…Using mathematical analysis of the measured spectral data, for example, partial least squares regression (PLSR), the authors derived acceptable prediction models for water and oil content and mentioned the possibility of obtaining good models to predict the protein content in their oilseed samples (Pedersen et al, 2000). Horn et al (2011) developed a fast, nondestructive method for simultaneous determination of oil and protein in cottonseed using a low-field 1 H time-domain (TD) NMR instrument. Horn et al (2011) developed a fast, nondestructive method for simultaneous determination of oil and protein in cottonseed using a low-field 1 H time-domain (TD) NMR instrument.…”

Section: Resonance Spectroscopymentioning

confidence: 99%

“…Weir et al (2005) reported the development of a rapid, nondestructive method for indirect protein content determination of soybean seeds based on NMR spectroscopy and claimed an adequate prediction ability of their method for breeders searching for high-protein soybean varieties. The authors reported that the oil and protein contents of cottonseed are negatively correlated, and they predicted the protein content of their samples using this weak negative correlation (Horn et al, 2011). The authors reported that the oil and protein contents of cottonseed are negatively correlated, and they predicted the protein content of their samples using this weak negative correlation (Horn et al, 2011).…”

Section: Resonance Spectroscopymentioning

confidence: 99%

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Advancing the science of wheat quality evaluation using nuclear magnetic resonance (NMR) and ultrasound‐based techniques

et al. 2018

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Background and objectives The need for cost‐effective, fast, and reliable techniques to evaluate wheat protein content and quality has led to the modification of available methods as well as the development of new methods. This review provides information on two emerging methods, that is, low‐field nuclear magnetic resonance (LF‐NMR) and low‐intensity ultrasound, with potential for evaluating wheat protein content and quality. Findings New techniques with additional capabilities have been developed over the years to provide wheat researchers with information that could not be acquired by the use of conventional methods. LF‐NMR and low‐intensity ultrasound are two emerging techniques in the world of cereal science whose speed, ease of operation, and reliability could promote their applications to wheat screening research studies. This review of these two potential wheat protein quality evaluation methods highlights the necessity of developing such cutting‐edge tools which are advantageous over the conventional tools. Conclusions Easy‐to‐use LF‐NMR and low‐intensity ultrasound seem to have the potential to provide nondestructive, fast evaluation methods that are superior to the currently used techniques in terms of accuracy and cost, respectively. However, further investigations to achieve complete development of such methods are still required. Significance and novelty The potential of LF‐NMR and low‐intensity ultrasound as fast, accurate, and cost‐effective wheat screening tools was confirmed through critical discussions and comparisons of a variety of most widely used wheat quality evaluation techniques.

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“…Mature intact seeds were loaded into the tubes at 20°C, and the spin-echo signal was averaged over 16 measurements. The signal height of the spin echo is proportional to lipid content in seeds Horn et al, 2011b), given that seeds are dry. The proportionality constant was estimated from a range of samples with known lipid content.…”

Section: High-throughput Quantification Of Oil Content Using Low-fielmentioning

confidence: 99%

A Noninvasive Platform for Imaging and Quantifying Oil Storage in Submillimeter Tobacco Seed

et al. 2012

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While often thought of as a smoking drug, tobacco (Nicotiana spp.) is now considered as a plant of choice for molecular farming and biofuel production. Here, we describe a noninvasive means of deriving both the distribution of lipid and the microtopology of the submillimeter tobacco seed, founded on nuclear magnetic resonance (NMR) technology. Our platform enables counting of seeds inside the intact tobacco capsule to measure seed sizes, to model the seed interior in three dimensions, to quantify the lipid content, and to visualize lipid gradients. Hundreds of seeds can be simultaneously imaged at an isotropic resolution of 25 mm, sufficient to assess each individual seed. The relative contributions of the embryo and the endosperm to both seed size and total lipid content could be assessed. The extension of the platform to a range of wild and cultivated Nicotiana species demonstrated certain evolutionary trends in both seed topology and pattern of lipid storage. The NMR analysis of transgenic tobacco plants with seed-specific ectopic expression of the plastidial phosphoenolpyruvate/phosphate translocator, displayed a trade off between seed size and oil concentration. The NMR-based assay of seed lipid content and topology has a number of potential applications, in particular providing a means to test and optimize transgenic strategies aimed at the manipulation of seed size, seed number, and lipid content in tobacco and other species with submillimeter seeds.

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Simultaneous Quantification of Oil and Protein in Cottonseed by Low‐Field Time‐Domain Nuclear Magnetic Resonance

Cited by 39 publications

References 18 publications

Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

Spatial Mapping of Lipids at Cellular Resolution in Embryos of Cotton

Advancing the science of wheat quality evaluation using nuclear magnetic resonance (NMR) and ultrasound‐based techniques

A Noninvasive Platform for Imaging and Quantifying Oil Storage in Submillimeter Tobacco Seed

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