Seed Bio-priming for Biotic and Abiotic Stress Management

Prasad, S. Rajendra; Kamble, Umesh; Sripathy, K. V.; Bhaskar, K. Udaya; Singh, D. P.

doi:10.1007/978-81-322-2647-5_12

Cited by 28 publications

(16 citation statements)

References 90 publications

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“…Besides the nature of crops and environmental conditions, the methods of inoculation influence the survival, multiplication, and colonization potential of microbial inoculants applied [51]. Among different methods of inoculation, seed treatments, seedlings treatments, foliar application, and soil treatments were widely used and found effective [52][53][54][55]. Seed bio-priming is a pre-sowing treatment which leads to a physiological state that enables microbial inoculants to establish a close contact with the seed [56,57].…”

Section: Introductionmentioning

confidence: 99%

Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.)

Singh

Malviya

et al. 2020

IJERPH

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Plant growth promoting rhizobacteria Pseudomonas aeruginosa strain MF-30 isolated from maize rhizosphere was characterized for several plant growth stimulating attributes. The strain MF-30 was also evaluated for antifungal properties against Rhizoctonia solani causing banded leaf and sheath blight in maize (Zea mays L.) under in vitro conditions and was found to have higher mycelial growth suppression in the culture suspension (67.41%) followed by volatile organic compounds (62.66%) and crude extract (51.20%) in a dual plate assay. The endophytic and epiphytic colonization ability was tested using Green Fluorescent Protein (GFP)-tagging. Visualization through confocal scanning laser microscope clearly indicated that strain MF-30 colonizes the root and foliar parts of the plants. Further, the effects of seed bio-priming with P. aeruginosa MF-30 was evaluated in the induction and bioaccumulation of defense-related biomolecules, enzymes, natural antioxidants, and other changes in maize under pot trial. This not only provided protection from R. solani but also ensured growth promotion under pathogenic stress conditions in maize. The maximum concentration of hydrogen peroxide (H2O2) was reported in the root and shoot of the plants treated with R. solani alone (8.47 and 17.50 mmol mg−1 protein, respectively) compared to bioagent, P. aeruginosa MF-30 bio-primed plants (3.49 and 7.50 mmol mg−1 protein, respectively). Effects on total soluble sugar content, total protein, and total proline were also found to enhanced significantly due to inoculation of P. aeruginosa MF-30. The activities of anti-oxidative defense enzymes phenylalanine ammonia lyase (PAL), ascorbate peroxidase, peroxidase, superoxide dismutase, and catalase increased significantly in the plants bio-primed with P. aeruginosa MF-30 and subsequent foliar spray of culture suspension of MF-30 compared to pathogen alone inoculated plants. qRT-PCR analysis revealed that seed bio-priming and foliar application of P. aeruginosa MF-30 significantly increased the expression of PR-1 and PR-10 genes with the simultaneous decrease in the disease severity and lesion length in the maize plants under pathogenic stress conditions. A significant enhancement of shoot and root biomass was recorded in MF-30 bio-primed plants as compared to untreated control (p < 0.05). Significant increase in plant growth and antioxidant content, as well as decreased disease severity in the P. aeruginosa MF-30 bio-primed plants, suggested the possibility of an eco-friendly and economical means of achieving antioxidants-rich, healthier maize plants.

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Section: Introductionmentioning

confidence: 99%

Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.)

Singh

Malviya

et al. 2020

IJERPH

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“…Biopriming is conceptualized as a technique of seed priming using living bacterial inoculum which allows bacterial adherence and acclimatization to the seeds in the prevalent conditions [ 18 ]. Seed biopriming provides a lot of benefits to plants especially in enhancing seed viability, germination, seed vigor, growth, and yield [ 19 ]. Bioprimed seeds have been proven to significantly enhance the germination percentage and the germination rate of seeds even under an induced environmental stress circumstance such as osmotic stress conditions [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

et al. 2020

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Limited information is available that seed biopriming with plant growth-promoting Enterobacter spp. play a prominent role to enhance vegetative growth of plants. Contrary to Enterobacter cloacae, Enterobacter hormaechei is a less-studied counterpart despite its vast potential in plant growth-promotion mainly through the inorganic phosphorus (P) and potassium (K) solubilization abilities. To this end, 18 locally isolated bacterial pure cultures were screened and three strains showed high P-and K-solubilizing capabilities. Light microscopy, biochemical tests and 16S rRNA gene sequencing revealed that strains 15a1 and 40a were closely related to Enterobacter hormaechei while strain 38 was closely related to Enterobacter cloacae (Accession number: MN294583; MN294585; MN294584). All Enterobacter spp. shared common plant growth-promoting traits, namely nitrogen (N 2) fixation, indole-3-acetic acid production and siderophore production. The strains 38 and 40a were able to produce gibberellic acid, while only strain 38 was able to secrete exopolysaccharide on agar. Under in vitro germination assay of okra (Abelmoschus esculentus) seeds, Enterobacter spp. significantly improved overall germination parameters and vigor index (19.6%) of seedlings. The efficacy of root colonization of Enterobacter spp. on the pre-treated seedling root tips was confirmed using Scanning Electron Microscopy (SEM). The pot experiment of bioprimed seeds of okra seedling showed significant improvement of the plant growth (> 28%) which corresponded to the increase of P and K uptakes (> 89%) as compared to the uninoculated control plants. The leaf surface area and the SPAD chlorophyll index of bioprimed plants were increased by up to 29% and 9% respectively. This report revealed that the under-explored species of P-and K-solubilizing Enterobacter hormaechei sp. with multiple plant beneficial traits presents a great potential sustainable approach for enhancement of soil fertility and P and K uptakes of plants.

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“…The unlikeliness of a regulatory framework would also hamper the development of products based on microbial consortia that exhibit complementary and synergistic effects, through re-assembling strains with differing modes of action into small communities, thereby providing more consistent protection or growth promotion than with the application of single strains, which are now starting to gain attention as a possible strategy to widen the application of bioproducts ( Reddy and Saravanan, 2013 ; Vassilev et al, 2015 ; De Vrieze et al, 2018 ). At the same time, the potential use of bioproducts for alleviating other abiotic stresses ( Hassen et al, 2016 ; Rajendra Prasad et al, 2016 ), particularly relevant in the world-wide experienced climate change conditions, would also face difficulties due to lack of clear rules for their registration and marketing. The current regulatory framework in EU as well as that of other countries where bioproducts are highly promoted (see several articles in Singh et al, 2016 ) could be perceived as frustrating the researchers efforts in finding the best solutions to exploit microbial inocula, considering that plants (and animals) are no longer viewed as autonomous entities, but rather as “holobionts” ( Bordenstein and Theis, 2015 ).…”

Section: Regulatory Future Perspectives Of Multifunctional Bioproductmentioning

confidence: 99%

Field Exploitation of Multiple Functions of Beneficial Microorganisms for Plant Nutrition and Protection: Real Possibility or Just a Hope?

et al. 2020

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Bioproducts, i.e., microbial based pesticides or fertilizers (biopesticides and biofertilizers), should be expected to play an ever-increasing role and application in agricultural practices worldwide in the effort to implement policies concerned with sustainable agriculture. However, several microbial strains have proven the capacity to augment plant productivity by enhancing crop nutrition and functioning as biopesticides, or vice-versa. This multifunctionality is an issue that is still not included as a concept and possibility in any legal provision regarding the placing on the market of bioproducts, and indicates difficulties in clearly classifying the purpose of their suitability. In this review, we overview the current understanding of the mechanisms in plant-microbe interactions underlining the dual function of microbial strains toward plant nutrition and protection. The prospects of market development for multifunctional bioproducts are then considered in view of the current regulatory approach in the European Union, in an effort that wants to stimulate a wider adoption of the new knowledge on the role played by microorganisms in crop production.

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Seed Bio-priming for Biotic and Abiotic Stress Management

Cited by 28 publications

References 90 publications

Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.)

Seed Biopriming with Microbial Inoculant Triggers Local and Systemic Defense Responses against Rhizoctonia solani Causing Banded Leaf and Sheath Blight in Maize (Zea mays L.)

Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

Field Exploitation of Multiple Functions of Beneficial Microorganisms for Plant Nutrition and Protection: Real Possibility or Just a Hope?

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