Reactive oxygen species have a dual role in seed physiology. Understanding the relative importance of beneficial and detrimental effects of ROS provides great scope for the improvement and maintenance of seed vigour and quality, factors that may ultimately increase crop yields.

Endophytes are non-disease causing microbes (bacteria and fungi) surviving in living tissues of plants. Their intimate association and possible coevolution with their plant partners have resulted in them contributing to an array of plant growth benefits ranging from enhanced growth and biomass accumulation, tolerance to abiotic and biotic stresses and in nutrient acquisition. The last couple of decades have witnessed a burgeoning literature on the role of endophytes (Class 3 type) in regulating plant growth and development and their adaptation to abiotic and biotic stresses. Though the underlying mechanisms of plant-endophyte interactions are far from clear, several studies have raised the hope of their potential application in agriculture, especially in mitigating abiotic and biotic stresses. The use of endophytes is envisaged as a route to reduce the production cost and burden on the environment by lessening the dependence on breeding for crop improvement and agrochemicals. Unfortunately, save a few well documented examples of their use, a little of these insights has been translated into actual agricultural applications. Here, we reflect on this paucity and elaborate on some of the important bottlenecks that might stand in way of fully realizing the potential that endophytes hold for crop improvement. We stress the need to study various facets of the endophyte-plant association for their gainful application in agriculture.

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Pre-sowing seed treatment with systemic fungicides is a firmly entrenched practice for most agricultural crops worldwide. The treatment is intended to protect the crop against seed- and soil-borne diseases. In recent years, there is increasing evidence that fungicidal applications to manage diseases might inadvertently also affect non-target organisms, such as endophytes. Endophytes are ubiquitously present in plants and contribute to plant growth and development besides offering resistance to biotic and abiotic stresses. In seeds, endophytes may play a role in seed development, seed germination, seedling establishment and crop performance. In this paper, we review the recent literature on non-target effects of fungicidal applications on endophytic fungal community and discuss the possible consequences of indiscriminate seed treatment with systemic fungicide on seed endophytes. It is now well recognized that endophytes are ubiquitously present in all parts of the plant, including the seeds. They may be transmitted vertically from seed to seed as in many grasses and/or acquired horizontally from the soil and the environment. Though the origins and evolution of these organisms in plants are a matter of conjecture, numerous studies have shown that they symbiotically aid in plant growth and development, in nutrient acquisition as well in protecting the plants from abiotic and biotic stresses. Against this background, it is reasonable to assume that the use of systemic fungicides in seed treatment may not only affect the seed endophytes but also their attendant benefits to seedling growth and establishment. While there is evidence to indicate that fungicidal applications to manage plant diseases also affect foliar endophytes, there are only few studies that have documented the effect of seed treatment on seed-borne endophytes. Some of the convincing examples of the latter come from studies on the effect of fungicide application on rye grass seed endophyte AR37. More recently, experiments have shown that removal of seed endophytes by treatment with systemic fungicides leads to significant loss of seedling vigour and that such losses could be partially restored by enriching the seedlings with the lost endophytes. Put together, these studies reinforce the importance of seed endophytes to seedling growth and establishment and draw attention on how to trade the balance between the benefits of seed treatments and the direct and indirect costs incurred due to loss of endophytes. Among several approaches, use of reduced-risk fungicides and identifying fungicide-resistant endophytes are suggested to sustain the endophyte contribution to early seedling growth.

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In two field experiments conducted over five successive sugarcane crops (including four ratoon crops), yield decline was associated with a decrease in the organic carbon content of the soil. Reduction in cane yield was minimized by the combined application of farmyard manure and urea, which resulted in an increase in soil organic carbon of 0.023% over the initial status within five years. Incorporation of farm waste materials such as cane trash, rice straw and legume husk into the soil before cane planting also increased the soil organic carbon content. Maximum benefits, however, were produced by the incorporation of legume husk with 150 kg N ha~'. A judicious mixture of chemical fertilizer and organic manures is therefore suggested as a means of sustaining the productivity of sugarcane. Materias organicas en el sueloy el rendimiento de cana de azucar RESUMENEn dos experimentos de campo realizados con cinco cosechas sucesivas de cana de azucar (incluyendo cuatro cosechas de retonos), la reduccion en el rendimiento de cultivo estuvo asociada con la reduccion en el contenido de carbono organico en el suelo. La reduccion en el rendimiento de cana fue minimizada por la aplicacion combinada de urea y abono de corral, lo cual resulto en un incremento del 0,023% en el contenido de carbono organico del suelo en relation con el estado inicial dentro de los cinco anos. La incorporacion al suelo de materiales de desecho agricola, tal como bagazo de cana, paja de arroz, y hollejos de legumbres tambien incremento el contenido de carbono organico del mismo. No obstante, el maximo beneficio se produjo con la incorporacion de hollejos de legumbres con 150 kg de N ha~'. Por lo tanto, se sugiere una mezcla razonable de fertilizante qufmico y abonos organicos para metodo para mantener la productividad de la cana de azucar.