Sedimentary arsenite-oxidizing and arsenate-reducing bacteria associated with high arsenic groundwater from Shanyin, Northwestern China

Fan, Haoxin; Su, Chunli; Wang, Yonghu; Yao, Jun; Zhao, Kai Hong; Wang, G.

doi:10.1111/j.1365-2672.2008.03790.x

Cited by 163 publications

(91 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, in the case of Sb(III) adsorbed onto goethite, oxidation only occurs in the light and was pH dependent, increasing at pH values of Ͼ5 (107). Since the Sb(III)-oxidizing bacteria reported so far have all been isolated from neutral-pH-range environments (31,(109)(110)(111)(112)(113)(114)(115), it appears that microbes may play a major role of Sb(III) oxidation at circumneutral pH.…”

Section: Microbial Antimony Cyclementioning

confidence: 99%

Microbial Antimony Biogeochemistry: Enzymes, Regulation, and Related Metabolic Pathways

Wang

Oremland

et al. 2016

Appl Environ Microbiol

152

View full text Add to dashboard Cite

e Antimony (Sb) is a toxic metalloid that occurs widely at trace concentrations in soil, aquatic systems, and the atmosphere. Nowadays, with the development of its new industrial applications and the corresponding expansion of antimony mining activities, the phenomenon of antimony pollution has become an increasingly serious concern. In recent years, research interest in Sb has been growing and reflects a fundamental scientific concern regarding Sb in the environment. In this review, we summarize the recent research on bacterial antimony transformations, especially those regarding antimony uptake, efflux, antimonite oxidation, and antimonate reduction. We conclude that our current understanding of antimony biochemistry and biogeochemistry is roughly equivalent to where that of arsenic was some 20 years ago. This portends the possibility of future discoveries with regard to the ability of microorganisms to conserve energy for their growth from antimony redox reactions and the isolation of new species of "antimonotrophs."

show abstract

Section: Microbial Antimony Cyclementioning

confidence: 99%

Microbial Antimony Biogeochemistry: Enzymes, Regulation, and Related Metabolic Pathways

Wang

Oremland

et al. 2016

Appl Environ Microbiol

152

View full text Add to dashboard Cite

show abstract

“…Some were closely related to AoxB sequences retrieved from various geographically distant areas throughout the world, showing that the related aoxB-carrying bacteria are ubiquitous in As-polluted environments. The exclusive detection of proteobacterial AoxB sequences has been reported for mesophilic environments (9,17,25), while most of the nonproteobacterial AoxB sequences (Chloroflexi, Aquificae, Deinococcus-Thermus, and Crenarchaeota phyla) came from thermophilic strains or geothermal environment surveys (7,14,17).…”

mentioning

confidence: 99%

“…They belong to more than 25 genera, mainly of the Proteobacteria phylum (3,32,38), and are related to organisms unable to oxidize As(III) based on 16S rRNA phylogeny. Diverse primer sets have been successfully developed to specifically target the functional aoxB gene (9,14,17,25,26), encoding the large molybdenum-bearing catalytic subunit of As(III)-oxidase (EC 1.20.98.1), an enzyme of the dimethyl sulfoxide (DMSO) reductase family. Using cloning-sequencing approaches, the aoxB gene has proven to be a reliable molecular marker for diversity studies of the polyphyletic aerobic As(III) oxidizers in As-impacted soil and water systems (17,25).…”

mentioning

confidence: 99%

Population Structure and Abundance of Arsenite-Oxidizing Bacteria along an Arsenic Pollution Gradient in Waters of the Upper Isle River Basin, France

Quéméneur

Cébron

Billard

et al. 2010

Appl Environ Microbiol

View full text Add to dashboard Cite

Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR (qPCR) were successfully developed to monitor functional aoxB genes as markers of aerobic arsenite oxidizers. DGGE profiles showed a shift in the structure of the aoxB-carrying bacterial population, composed of members of the Alpha-, Beta-and Gammaproteobacteria, depending on arsenic (As) and E h levels in Upper Isle River Basin waters. The highest aoxB gene densities were found in the most As-polluted oxic surface waters but without any significant correlation with environmental factors. Arsenite oxidizers seem to play a key role in As mobility in As-impacted waters.Arsenic (As) occurs naturally as a local geological constituent of the soils surrounding the Upper Isle River Basin (Massif Central, France) due to natural geochemical anomalies but is also released from Au/As deposits of disused gold mines (4,11,12,33). Important variations in dissolved As concentrations are found in the Isle River and depend on the hydrogeological season, with maximum values in spring and summer generally detected during low-flow conditions (12,22), and probably on temperature-controlled microbial As(V) reduction and/or microbial dissolution of solid As carrier phases (22). Two toxic inorganic forms of As are usually detected in aquatic system: arsenite, As(III), which is found mainly under anaerobic conditions and is more mobile than arsenate, As(V), which typically occurs under aerobic conditions and tends to associate with oxyhydroxides and clay minerals (11,34). Although bacteria are known to play a key role in speciation, mobility, and bioavailability of As in the environment, they have never been considered in previous studies of As mobility in the Isle River system. Indeed, former investigations of As cycling were focused on geochemical studies (4,11,12,22,33).As(III)-oxidizing bacteria can contribute to a natural attenuation of As pollution by decreasing its bioavailability and can help remove As from mine wastewaters through bioprocessing (1, 2). Many As(III) oxidizers have been isolated from various environments, especially mesophilic ecosystems (3,5,8,16,25,27,32,38). They belong to more than 25 genera, mainly of the Proteobacteria phylum (3, 32, 38), and are related to organisms unable to oxidize As(III) based on 16S rRNA phylogeny. Diverse primer sets have been successfully developed to specifically target the functional aoxB gene (9,14,17,25,26), encoding the large molybdenum-bearing catalytic subunit of As(III)-oxidase (EC 1.20.98.1), an enzyme of the dimethyl sulfoxide (DMSO) reductase family. Using cloning-sequencing approaches, the aoxB gene has proven to be a reliable molecular marker for diversity studies of the polyphyletic aerobic As(III) oxidizers in As-impacted soil and water systems (17, 25). The genetic fingerprinting denaturing gradient gel electrophoresis (DGGE) technique is one useful tool for spatial, temporal, and geographical monitoring of complex bacterial population structure (23, 24). Quantitative real-time PCR (qPCR) p...

show abstract

“…Most of these strains are facultative chemolithoautotrophic arsenite oxidizers, and were isolated from arsenic-contaminated environments such as gold mine, 17,[31][32][33] hot creek, 34) and sediment. 35) In this study, we have newly isolated a facultative arsenite-oxidizing bacterium strain KGO-5 from arsenic-contaminated industrial soil, and it was phylogenetically closely related with Sinorhizobium meliloti with 16S rRNA gene sequence similarity of 99.3%. Although arsenite-oxidizing bacteria related with Sinorhizobium spp.…”

Section: Discussionmentioning

confidence: 96%

Arsenite oxidation by a facultative chemolithoautotrophicSinorhizobiumsp. KGO-5 isolated from arsenic-contaminated soil

Dong

Ohtsuka

Dong

et al. 2014

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

A chemolithoautotrophic arsenite-oxidizing bacterium, designated strain KGO-5, was isolated from arsenic-contaminated industrial soil. Strain KGO-5 was phylogenetically closely related with Sinorhizobium meliloti with 16S rRNA gene similarity of more than 99%, and oxidized 5 mM arsenite under autotrophic condition within 60 h with a doubling time of 3.0 h. Additions of 0.01–0.1% yeast extract enhanced the growth significantly, and the strain still oxidized arsenite efficiently with much lower doubling times of approximately 1.0 h. Arsenite-oxidizing capacities (11.2–54.1 μmol h−1 mg dry cells−1) as well as arsenite oxidase (Aio) activities (1.76–10.0 mU mg protein−1) were found in the cells grown with arsenite, but neither could be detected in the cells grown without arsenite. Strain KGO-5 possessed putative aioA gene, which is closely related with AioA of Ensifer adhaerens. These results suggest that strain KGO-5 is a facultative chemolithoautotrophic arsenite oxidizer, and its Aio is induced by arsenic.

show abstract

Sedimentary arsenite-oxidizing and arsenate-reducing bacteria associated with high arsenic groundwater from Shanyin, Northwestern China

Cited by 163 publications

References 33 publications

Microbial Antimony Biogeochemistry: Enzymes, Regulation, and Related Metabolic Pathways

Microbial Antimony Biogeochemistry: Enzymes, Regulation, and Related Metabolic Pathways

Population Structure and Abundance of Arsenite-Oxidizing Bacteria along an Arsenic Pollution Gradient in Waters of the Upper Isle River Basin, France

Arsenite oxidation by a facultative chemolithoautotrophicSinorhizobiumsp. KGO-5 isolated from arsenic-contaminated soil

Contact Info

Product

Resources

About