Arsenite oxidation by a facultative chemolithoautotrophic<i>Sinorhizobium</i>sp. KGO-5 isolated from arsenic-contaminated soil

Dong, Dan Hong; Ohtsuka, Toshihiko; Dong, Dian Tao; Amachi, Seigo

doi:10.1080/09168451.2014.940276

Cited by 13 publications

(8 citation statements)

References 40 publications

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“…NT-26 and Sinorhizobium sp. KGO-5, where additions of yeast extract enhanced growth and accelerated As III oxidizing significantly ( Santini et al, 2000 ; Dong et al, 2014 ). Although the cell growth was enhanced due to the relatively higher optical densities, no obvious As III oxidization has been observed during the logarithmic phase ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

Chemolithoautotrophic arsenite oxidation by a thermophilic Anoxybacillus flavithermus strain TCC9-4 from a hot spring in Tengchong of Yunnan, China

Jiang

et al. 2015

Front. Microbiol.

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A new facultative chemolithoautotrophic arsenite (AsIII)-oxidizing bacterium TCC9-4 was isolated from a hot spring microbial mat in Tengchong of Yunnan, China. This strain could grow with AsIII as an energy source, CO2–HCO3- as a carbon source and oxygen as the electron acceptor in a minimal salts medium. Under chemolithoautotrophic conditions, more than 90% of 100 mg/L AsIII could be oxidized by the strain TCC9-4 in 36 h. Temperature was an important environmental factor that strongly influenced the AsIII oxidation rate and AsIII oxidase (Aio) activity; the highest Aio activity was found at the temperature of 40∘C. Addition of 0.01% yeast extract enhanced the growth significantly, but delayed the AsIII oxidation. On the basis of 16S rRNA phylogenetic sequence analysis, strain TCC9-4 was identified as Anoxybacillus flavithermus. To our best knowledge, this is the first report of arsenic (As) oxidation by A. flavithermus. The Aio gene in TCC9-4 might be quite novel relative to currently known gene sequences. The results of this study expand our current understanding of microbially mediated As oxidation in hot springs.

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Section: Resultsmentioning

confidence: 99%

Chemolithoautotrophic arsenite oxidation by a thermophilic Anoxybacillus flavithermus strain TCC9-4 from a hot spring in Tengchong of Yunnan, China

Jiang

et al. 2015

Front. Microbiol.

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“…Alphaproteobacterial As(III)-oxidizing bacteria, including Bosea sp. WAO, have frequently been isolated from various environments ( 7 , 11 , 22 , 37 , 39 , 40 ). Many of these bacteria are facultative chemolithoautotrophic As(III) oxidizers and are highly resistant to As(III) levels of more than 5,000 μM.…”

Section: Discussionmentioning

confidence: 99%

“…Our results suggest that exposure to high As(III) levels has a significant impact on the structure and diversity of the As(III)-oxidizing bacterial community in soil, and that certain As(III)-oxidizing bacteria with strong As(III) resistance are enriched specifically under these conditions. The complete oxidation of 5,000 μM As(III) generally yields sufficient metabolic energy for growth under chemolithoautotrophic conditions ( 7 , 22 , 37 , 39 ). Hence, we speculate that Bosea- like As(III)-oxidizing bacteria also increased their biomass in slurry spiked with 5,000 μM As(III).…”

Section: Discussionmentioning

confidence: 99%

Impact of Arsenite on the Bacterial Community Structure and Diversity in Soil

2016

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The impact of arsenite (As[III]) on the bacterial community structure and diversity in soil was determined by incubating soil slurries with 50, 500, and 5,000 μM As(III). As(III) was oxidized to arsenate (As[V]), and the microbial contribution to As(III) oxidation was 70–100%. PCR-denaturing gradient gel electrophoresis revealed that soil bacterial diversity decreased in the presence of As(III). Bacteria closely related to the family Bacillaceae were predominant in slurry spiked with 5,000 μM As(III). The population size of culturable As(III)-resistant bacteria was 37-fold higher in this slurry than in unspiked slurry (p < 0.01), indicating that high levels of As(III) stimulate the emergence of As(III)-resistant bacteria. As(III)-resistant bacteria isolated from slurry spiked with 5,000 μM As(III) were mainly affiliated with the genus Bacillus; however, no strains showed As(III)-oxidizing capacity. An As(III)-oxidizing bacterial community analysis based on As(III) oxidase gene (aioA) sequences demonstrated that diversity was the lowest in slurry spiked with 5,000 μM As(III). The deduced AioA sequences affiliated with Alphaproteobacteria accounted for 91–93% of all sequences in this slurry, among which those closely related to Bosea spp. were predominant (48–86%). These results suggest that exposure to high levels of As(III) has a significant impact on the composition and diversity of the soil bacterial community, including the As(III)-oxidizing bacterial community. Certain As(III)-oxidizing bacteria with strong As(III) resistance may be enriched under high As(III) levels, while more sensitive As(III) oxidizers are eliminated under these conditions.

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“…Thus, phenomena linked to high concentrations of metals and arsenic may have attenuated the decrease of redox potential linked to oxygen consumption in the saturated level, while OM was provided, compared with less polluted environments. Up to now, all As(III)-oxidizing bacteria isolated from soils were heterotrophs or facultative autotrophs (Inskeep et al, 2007;Bachate et al, 2012;Bahar et al, 2013;Dong et al, 2014). The increasing amount of OM and its quality therefore promoted the growth of As(III)-oxidizing microorganisms.…”

Section: Effect Of Water Saturation and Addition Of Litter On Arsenicmentioning

confidence: 99%

Influence of environmental changes on the biogeochemistry of arsenic in a soil polluted by the destruction of chemical weapons: A mesocosm study

Thouin

Battaglia-Brunet

Norini

et al. 2018

Science of The Total Environment

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Thermal destruction of chemical munitions from World War I led to the formation of a heavily contaminated residue that contains an unexpected mineral association in which a microbial As transformation has been observed. A mesocosm study was conducted to assess the impact of water saturation episodes and input of bioavailable organic matter (OM) on pollutant behavior in relation to biogeochemical parameters. Over a period of about eight (8) months, the contaminated soil was subjected to cycles of dry and wet periods corresponding to water table level variations. After the first four (4) months, fragmented litter from the nearby forest was placed on top of the soil. The mesocosm solid phase was sampled by three rounds of coring: at the beginning of the experiment, after four (4) months (before the addition of OM), and at the end of the experiment. Scanning electron microscopy coupled to energy dispersive X-ray spectroscopy observations showed that an amorphous phase, which was the primary carrier of As, Zn, and Cu, was unstable under water-saturated conditions and released a portion of the contaminants in solution. Precipitation of a lead arsenate chloride mineral, mimetite, in soils within the water saturated level caused the immobilization of As and Pb. Mimetite is a durable trap because of its large stability domain; however, this precipitation was limited by a low Pb concentration inducing that high amounts of As remained in solution. The addition of forest litter modified the quantities and qualities of soil OM. Microbial As transformation was affected by the addition of OM, which increased the concentration of both As(III)-oxidizing and As(V)-reducing microorganisms. The addition of OM negatively impacted the As(III) oxidizing rate, however As(III) oxidation was still the dominant reaction in accordance with the formation of arsenate-bearing minerals.

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Arsenite oxidation by a facultative chemolithoautotrophicSinorhizobiumsp. KGO-5 isolated from arsenic-contaminated soil

Cited by 13 publications

References 40 publications

Chemolithoautotrophic arsenite oxidation by a thermophilic Anoxybacillus flavithermus strain TCC9-4 from a hot spring in Tengchong of Yunnan, China

Chemolithoautotrophic arsenite oxidation by a thermophilic Anoxybacillus flavithermus strain TCC9-4 from a hot spring in Tengchong of Yunnan, China

Impact of Arsenite on the Bacterial Community Structure and Diversity in Soil

Influence of environmental changes on the biogeochemistry of arsenic in a soil polluted by the destruction of chemical weapons: A mesocosm study

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