Section III: High magnification scanning electron microscopy

Peters, Klaus‐Ruediger

doi:10.1002/jemt.1060040205

Cited by 10 publications

(9 citation statements)

References 29 publications

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“…Cryo-HRSEM was employed to evaluate the morphology of hydrated electrospun fiber networks [18, 21, 40]. By raising the specimen stage temperature from −170 to −85°C, water was sublimated from the surface of the specimen.…”

Section: Resultsmentioning

confidence: 99%

A Permanent Change in Protein Mechanical Responses Can be Produced by Thermally-Induced Microdomain Mixing

Sallach

Leisen

Caves

et al. 2009

Journal of Biomaterials Science, Polymer Edition

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Electrospinning was employed to fabricate three dimensional fiber networks from a recombinant amphiphilic elastin-mimetic triblock protein polymer and the effects of moderate thermal conditioning (60°C, 4h) on network mechanical responses investigated. Significantly, while cryo-high resolution scanning electron microscopy (cryo-HRSEM) revealed that macroscopic and microscopic morphology of the network structure was unchanged, solid-state 1H NMR spectroscopy demonstrated enhanced interphase mixing of hydrophobic and hydrophilic blocks. Significantly, thermal annealing triggered permanent changes in network swelling behavior (28.75 ± 2.80 non-annealed vs. 13.55 ± 1.39 annealed; p < 0.05) and uniaxial mechanical responses, including Young’s modulus (0.170 ± 0.010 MPa non-annealed vs. 0.366 ± 0.05 MPa annealed; p < 0.05) and ultimate tensile strength (0.079 ± 0.008 MPa vs 0.119 ± 0.015 MPa; p < 0.05). To our knowledge, these investigations are the first to note that mechanical responses of protein polymers can be permanently altered through a temperature-induced change in microphase mixing.

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Section: Resultsmentioning

confidence: 99%

A Permanent Change in Protein Mechanical Responses Can be Produced by Thermally-Induced Microdomain Mixing

Sallach

Leisen

Caves

et al. 2009

Journal of Biomaterials Science, Polymer Edition

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“…3) the shadow-casting is made at an oblique angle, which means that the topology of the specimen surface can be obscured because of variations in thickness of the Pt-C-film (Huyat 1972, Koehler 1973, Peters 1987). An advantage, however, with the replica-technique is the use of freeze-dried tissue being less shrunken than critial point dried samples (Boyde 1978).…”

Section: Discussionmentioning

confidence: 99%

TEM‐, SEM‐ and STEM‐studies of sputter‐coated cytoskeletons

1987

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A technique for sputter coating of cytoskeletons from detergent extracted cells is described. The method allows their study in high resolution TEM, SEM and STEM. The use of cytoskeletons is also a valuable model system for the evaluation of metal coating techniques, allowing studies of deposited metal on fine filamentous structures of varying thicknesses.Pt and W were sputtered at 1 to 5 nm, and comparisons were made with reference to contrast and granularity. Both metals gave good topographical contrast, but Pt showed a coarse structure and a greater decorating tendency than W.The method provides a simple system for studies of the three-dimensional cytoskeletal organization, without the use of cumbersome replica-techniques.

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“…Research has shown that Cr deposited from a cold Penning ionization source provides the most conformal coatings [42] and this approach has also been used to improve the imaging of catalytic materials [43]. The smallest resolvable distance between two Au islands is considered the microscope resolution.…”

Section: F Scanning Electron Microscopymentioning

confidence: 99%

Electron Microscopy and Diffraction

Datye

Hansen

Helveg

2008

Handbook of Heterogeneous Catalysis

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The sections in this article are Introduction Imaging Electron Diffraction Electron Beam Microanalysis In Situ Electron Microscopy Summary and Conclusions Acknowledgments

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Section III: High magnification scanning electron microscopy

Cited by 10 publications

References 29 publications

A Permanent Change in Protein Mechanical Responses Can be Produced by Thermally-Induced Microdomain Mixing

A Permanent Change in Protein Mechanical Responses Can be Produced by Thermally-Induced Microdomain Mixing

TEM‐, SEM‐ and STEM‐studies of sputter‐coated cytoskeletons

Electron Microscopy and Diffraction

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