Secretion of three enzymes for fatty acid synthesis into mouse milk in association with fat globules, and rapid decrease of the secreted enzymes by treatment with rapamycin

Kinetics of milk lipid droplet transport, growth, and secretion revealed by intravital imaging: lipid droplet release is intermittently stimulated by oxytocin

“…We saw no evidence for the rapid expansion of individual LDs (>0.7 μm diameter) in the absence of discrete cytoplasmic BODIPY-stained precursors, for example, through the activity of lipid synthetic enzymes in situ (Fujimoto et al. , 2007; Moriya, Uchida, et al. , 2011) or evidence for the fragmentation of preexisting droplets, that is, droplet expansion appeared to be a unidirectional process.…”

“…, 1984; Deeney et al. , 1985) and possibly by synthetic expansion in situ (Moriya, Uchida, et al. , 2011).…”

Identification of lipid synthesis and secretion proteins in bovine milk

“…This may explain the presence of FASN in MFGM (Cebo et al 2010). Moriya et al (2011) also suggested that in mouse, ACACA and FASN were localised in the ER membrane and the cytoplasmic MFG precursor surface, and therefore are secreted together with MFG to milk to increase the speed and efficiency of triglycerides synthesis. Two components of the FA-desaturase system, Acyl-CoA desaturase (SCD, Table 1) and NAPH-cytochrome b5 reductase (CYB5R3, Table 1), were found in MFGM by using our proteomics analysis.…”

The murine Gcap14 gene encodes a novel microtubule binding and bundling protein

“…Cells were subjected to the complete MT depolymerization described above, lysed with 50 mM Tris–HCl, pH 7.5, containing 0.15 M NaCl, 1% NP‐40, 1 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, 50 μM proteasome inhibitor I (PSI), 10 μg/ml aprotinin, 5 μg/ml leupeptin, and 5 μg/ml pepstatin A, and centrifuged twice at 20 000 g for 15 min at 4 °C. The subsequent procedures were performed using the supernatants, appropriate antibodies, and protein G‐conjugated beads, essentially according to our previous report [12].…”

“…The mammary gland has a unique developmental cycle: cell proliferation in response to pregnancy, differentiation of the epithelial cells for milk secretion, and a regressive phase after weaning (involution), including extensive death of the secretory epithelial cells and tissue remodeling, for returning to a pre‐pregnant state [6–8]. To know the mechanisms underlying this mammary cycle, we have screened murine genes potentially included in these events in the cycle [9–12]. During the course of this screening, we have identified a previously uncharacterized gene, Gcap14.…”