Identification of lipid synthesis and secretion proteins in bovine milk

Lü, Jing; Hooijdonk, Toon van; Boeren, Sjef; Vervoort, Jacques; Hettinga, Kasper

doi:10.1017/s0022029913000642

Cited by 23 publications

(14 citation statements)

References 57 publications

(78 reference statements)

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“…Many of these minor proteins are post-translational modified proteins and have their own biological functions. [28][29][30] Although very low in quantity, they may provide significant beneficial activities for infant in immune system and cognitive functions. 28,31,32…”

Section: Resultsmentioning

confidence: 99%

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

et al. 2019

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BACKGROUND Xinong Saanen goat milk is a raw material for goat milk‐based infant formula production. This study aims to analyze digestion properties of Xinong Saanen goat colostrum and mature milk by simulating infant gastrointestinal digestion. Zeta potential, particles size, protein profile and peptides composition of these two kinds of milk during the digestion process were studied. RESULTS Zeta‐potential values of the digested colostrum were lower than those of mature milk through the whole digestion. Absolute zeta potential of colostrum duodenal digestion samples showed a decrease from 16.63 ± 2.08 to 11.80 ± 2.03 mV while that of mature milk decreased sharply and then increased (P < 0.05). Colostrum had a larger particle size than mature milk and both milks showed decreased particle size with increasing digestion time but an increase for the last 30 min. Colostrum showed more high molecular weight (MW) proteins which cannot be hydrolyzed completely compared with mature milk. Digested peptides (< 10 kDa) were characterized using liquid chromatography combined with tandem mass spectrometry (LC–MS/MS). The casein‐derived peptides identified in digested colostrum and mature milk accounted for 76.67% and 59.53%, respectively. β‐Casein was the most abundant in colostrum while that in mature milk was αs1‐casein. Enterotoxin‐binding glycoprotein PP20K, butyrophilin subfamily 1 member A1 (BTN1A1) and perilipin (PLIN) were only detected in digested mature milk. CONCLUSION Differences in digestion properties between goat colostrum and mature milk were mainly shown in duodenal digestion phase. Data may provide useful information about utilization of goat milk for infant formula formulation. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

et al. 2019

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“…The higher number of quantified MFGM proteins than milk serum proteins in both human and bovine (data set 1) is consistent with the numbers of identified proteins reported previously [ 8 ]. It is not surprising, as MFGM represent the epithelial cell, the place where the milk fat is synthesised and secreted [ 26 , 27 ]. The low amount of transport proteins in human milk can be mainly related to the absence of the major transport protein β-lactoglobulin (LGB) in human milk [ 28 ], which is the most abundant protein in bovine milk serum.…”

Section: Discussionmentioning

confidence: 99%

An interactomics overview of the human and bovine milk proteome over lactation

2016

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BackgroundMilk is the most important food for growth and development of the neonate, because of its nutrient composition and presence of many bioactive proteins. Differences between human and bovine milk in low abundant proteins have not been extensively studied. To better understand the differences between human and bovine milk, the qualitative and quantitative differences in the milk proteome as well as their changes over lactation were compared using both label-free and labelled proteomics techniques. These datasets were analysed and compared, to better understand the role of milk proteins in development of the newborn.MethodsHuman and bovine milk samples were prepared by using filter-aided sample preparation (FASP) combined with dimethyl labelling and analysed by nano LC LTQ-Orbitrap XL mass spectrometry.ResultsThe human and bovine milk proteome show similarities with regard to the distribution over biological functions, especially the dominant presence of enzymes, transport and immune-related proteins. At a quantitative level, the human and bovine milk proteome differed not only between species but also over lactation within species. Dominant enzymes that differed between species were those assisting in nutrient digestion, with bile salt-activated lipase being abundant in human milk and pancreatic ribonuclease being abundant in bovine milk. As lactation advances, immune-related proteins decreased slower in human milk compared to bovine milk. Notwithstanding these quantitative differences, analysis of human and bovine co-expression networks and protein-protein interaction networks indicated that a subset of milk proteins displayed highly similar interactions in each of the different networks, which may be related to the general importance of milk in nutrition and healthy development of the newborn.ConclusionsOur findings promote a better understanding of the differences and similarities in dynamics of human and bovine milk proteins, thereby also providing guidance for further improvement of infant formula.Electronic supplementary materialThe online version of this article (doi:10.1186/s12953-016-0110-0) contains supplementary material, which is available to authorized users.

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“…The MFGM contains a unique composition of polar lipids and membrane proteins which not only is intriguing as a model to study membrane lipids and proteins but function as markers of biological processes of the cow’s udder cells [ 139 ]. For example, Reinhardt et al [ 49 ] reported accumulation of host defense proteins and presence of NETs in MFGM preparations indicative of biology and immune function of the infected mammary gland [ 49 , 140 – 143 ]. Similarly, comparative profiling of milk lipids and proteins in healthy versus disease conditions showed contrasting expression of serpin A3-1, vitronectin-like protein and complement factor H [ 8 , 144 ].…”

Section: Alternate Diagnostic Body Fluidmentioning

confidence: 99%

Challenges and opportunities of bovine milk analysis by mass spectrometry

Verma

Ambatipudi

2016

Clin Proteom

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Bovine milk and its products (e.g. cheese, yoghurt) are an important part of human diet with beneficial effects for all ages. Although analyses of different milk components (e.g. proteins, lipids) pose huge challenges, the use of mass spectrometric (MS)-based techniques is steadily improving our understanding of the complexity of the biological traits that effect milk yield and its components to meet the global demand arising from population growth. In addition, different milk constituents have various applications in veterinary research and medicine, including early disease diagnosis. The aim of the review is to present an overview of the progress made in MS-based analysis of milk, and suggest a multi-pronged MS strategy to better explore different milk components for translational and clinical utilities.

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Identification of lipid synthesis and secretion proteins in bovine milk

Cited by 23 publications

References 57 publications

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

Protein digestion properties of Xinong Saanen goat colostrum and mature milk usingin vitrodigestion model

An interactomics overview of the human and bovine milk proteome over lactation

Challenges and opportunities of bovine milk analysis by mass spectrometry

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