The murine Gcap14 gene encodes a novel microtubule binding and bundling protein

Hosono, Hitomi; Yamaguchi, Nobuhisa; Oshima, Kenzi; Matsuda, Tsukasa; Nadano, Daita

doi:10.1016/j.febslet.2012.04.008

Cited by 3 publications

(2 citation statements)

References 23 publications

(38 reference statements)

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“…Given that the deficiency of Gcap14 reduced the mobile microtubule fraction without changing the half-maximal time, as shown in our FRAP analysis ( Fig. 3 E – I ), and that Gcap14 also directly binds to unpolymerized tubulin and microtubule ( 34 ), it is likely that Gcap14 takes part in tethering tubulins to microtubules to enhance the pool of dynamic microtubules in the multiple steps of neurodevelopment.…”

Section: Discussionmentioning

confidence: 58%

“…Additionally, according to the Biological General Repository for Interaction Datasets and STRING ( 32 , 33 ), the majority of Gcap14 interacting partners are functionally related to brain development and regulation of the microtubule cytoskeleton. Gcap14 has been reported to be highly expressed in the brain and binds to microtubules ( 34 ), but its neural functions remain unknown. Here, we identified that Gcap14 is a +TIP that functions as a regulator of microtubule dynamics, thereby modulating neocortical development.…”

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confidence: 99%

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Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule–actin crosstalk during neurodevelopment

Mun

Goo

Suh

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Regulation of microtubule dynamics is required to properly control various steps of neurodevelopment. In this study, we identified granule cell antiserum-positive 14 (Gcap14) as a microtubule plus-end-tracking protein and as a regulator of microtubule dynamics during neurodevelopment. Gcap14 knockout mice exhibited impaired cortical lamination. Gcap14 deficiency resulted in defective neuronal migration. Moreover, nuclear distribution element nudE-like 1 (Ndel1), an interacting partner of Gcap14, effectively corrected the downregulation of microtubule dynamics and the defects in neuronal migration caused by Gcap14 deficiency. Finally, we found that the Gcap14–Ndel1 complex participates in the functional link between microtubule and actin filament, thereby regulating their crosstalks in the growth cones of cortical neurons. Taken together, we propose that the Gcap14–Ndel1 complex is fundamental for cytoskeletal remodeling during neurodevelopmental processes such as neuronal processes elongation and neuronal migration.

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Section: Discussionmentioning

confidence: 58%

mentioning

confidence: 99%

Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule–actin crosstalk during neurodevelopment

Mun

Goo

Suh

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Lyar, a cell growth-regulating zinc finger protein, was identified to be associated with cytoplasmic ribosomes in male germ and cancer cells

et al. 2014

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Translational control is a basic mechanism for gene regulation in cells and important for tissue growth and development in mammals. Deregulation of the mechanism thus causes diseases such as cancer. Considering the importance of the ribosome as a factory of polypeptide synthesis, some new factors have been expected to be associated with the ribosome and involved in translational control. Our proteomic survey for these factors identified a zinc finger protein, Lyar, in cytoplasmic ribosomes of the rodent testis. Subcellular fractionation of the testis provided data supporting association of Lyar with ribosomes. Lyar was then suggested to be included in the 60S large subunit, but not in polysomes, by ultracentrifugation of testicular ribosomes. While analysis of tissue distribution of Lyar has indicated its testis-predominant expression, Lyar mRNA was expressed in the cancer cells originated from tissues other than testis, and Lyar promoted proliferation of NIH-3T3 cells. Furthermore, translation was increased by Lyar in vitro, pointing out the first experimental link between this protein and translation. Taken together, Lyar seems to be a new player in translational control and a potential target for cancer therapy.

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Characterization of human Ccser2 as a protein tracking the plus-ends of microtubules

Shirai,

Okuda,

Oshima

et al. 2023

BMC Res Notes

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Objective Microtubules, which are closely related to cell proliferation, have been the promising therapeutic target of cancer. Therefore, it is necessary to understand the intracellular control mechanisms of microtubules, the whole picture of which is still unclear though. Intracellular dynamics of microtubules are regulated by various microtubule-associated proteins, one group of which is microtubule plus-end-tracking proteins (+ TIPs), localizing to the extending tips of microtubules. Here, we report the identification and analysis of Ccser2 as a new + TIP in human breast cancer MCF-7 cells. Results Ccser2 was found to be a member of + TIPs by microscopic observations including time-lapse imaging. The C-terminal region of Ccser2, including two SxIP motifs, was likely to be important for the tracking function. In MCF-7 cells, endogenous Ccser2 was mainly detected in the peripheral regions of microtubule fibers, suggesting that Ccser2 functions in cell projections.

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The murine Gcap14 gene encodes a novel microtubule binding and bundling protein

Cited by 3 publications

References 23 publications

Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule–actin crosstalk during neurodevelopment

Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule–actin crosstalk during neurodevelopment

Lyar, a cell growth-regulating zinc finger protein, was identified to be associated with cytoplasmic ribosomes in male germ and cancer cells

Characterization of human Ccser2 as a protein tracking the plus-ends of microtubules

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