Secondary pollen types characteristic of Louisiana honeys

Lieux, Meredith Hoag

doi:10.1007/bf02866580

Cited by 10 publications

(7 citation statements)

References 3 publications

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“…Taxa of pollen are used to indicate the floral nectar sources utilized by bees to produce honey [ 7 , 8 , 9 , 10 ] and are also an essential tool to indicate the geographic origin of the honey [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Melissopalynological Characterization of North Algerian Honeys

Nair

Meddah

Aoues

2013

Foods

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A pollen analysis of Algerian honey was conducted on a total of 10 honey samples. The samples were prepared using the methodology described by Louveaux et al., that was then further adapted by Ohe et al. The samples were subsequently observed using light microscopy. A total of 36 pollen taxa were discovered and could be identified in the analyzed honey samples. Seventy percent of the studied samples belonged to the group ofmonofloral honeys represented by Eucalyptus globulus, Thymus vulgaris, Citrus sp. and Lavandula angustifolia. Multifloral honeys comprised 30% of the honey samples, with pollen grains of Lavandula stoechas (28.49%) standing out as the most prevalent. Based on cluster analysis, two different groups of honey were observed according to different pollen types found in the samples. The identified pollen spectrum of honey confirmed their botanical origin.

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Section: Introductionmentioning

confidence: 99%

Melissopalynological Characterization of North Algerian Honeys

Nair

Meddah

Aoues

2013

Foods

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“…Pollen analysis of honey is used to determine a honey's floral type, quality, and origin (Barth 1990, Deans 1957, Feller-Demalsy et al 1987a1989, Lieux 1972, 1977, 1978Louveaux 1956, Maurizio 1951Maurizio & Louveaux 1965, White et al 1962. In many countries, import and export laws require three types of certification including verification of the honey's floral type, quality, and precise place of origin.…”

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confidence: 99%

The use of ETOH for the dilution of honey

Jones

Bryant

2004

Grana

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2004. The use of ETOH for the dilution of honey. -Grana 43: 174-182. ISSN 0017-3134.Since the beginning of honey production, certain honey types are preferred because they taste better, are better for cooking, or do not rapidly crystallize. Pollen found in honey is used to determine the honey's type. Techniques used to extract pollen from honey vary in the amount of honey examined, the amount of water used to dilute the honey, and the time and speed of centrifugation. These variations address the disparity in pollen recovery that is due to the specific gravity and sinking rates of the different pollen types. Ethyl alcohol (ETOH) was used as a dilution liquid for honey and compared to two water-dilution techniques, one with a short centrifugation time (1 minute) and the other using a long centrifugation time (10 minutes). The ETOH samples were centrifuged for 3 minutes. All samples were centrifuged at 4000 r.p.m. Significantly higher pollen concentration values were found in the ETOH-diluted samples. Pollen concentration values of the ETOH-diluted samples were 5.26 times greater than water-diluted, short centrifugation samples, and 3.26 times greater than water-diluted, long centrifugation samples. ETOH-diluted samples produced 16% more taxa than the water-diluted short centrifugation technique, and 10% more taxa than the water-diluted long centrifugation technique. The pollen spectra were not consistent across the three techniques. Additional research is needed to determine the efficiency of other ETOH concentrations in recovering pollen from honey. We recommend that the ETOH-dilution technique become the standard technique for the extraction of pollen from honey for pollen analyses because of the improved pollen recovery.

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“…The identification of the pollen types was based on the European and Asian pollen flora catalogs (Punt & Clarke 1974, Lieux 1975-1977-1978, Göschl 2008, Haiqing 2015, among many publications that were consulted and that are part of the collection from the Palynological Research Centre (NPP) at the Institute of Botany, as well as others obtained from the Internet. The "Northwest European Pollen Flora" (Punt & Clarke 1974) is the world´s most detailed pollen morphological study and covers around 130 families and ca.…”

Section: Methodsmentioning

confidence: 99%

“…The samples were evaluated in two ways to identify its origin: 1) among samples, to verify its similarity, including all the pollen types and their absolute value and, 2) with those from other countries, to compare the pollen types found in each sample together with the presence or absence in royal jelly samples from Brazil (Barth 2005, Morgado & Barth 2011, China (Piana et al 2006), France (Stocker 2005), Greece (Dimou et al 2013) and Italy (Piana et al 2006) as well as Louisiana honey, United States (Lieux 1975(Lieux -1977(Lieux -1978. This allowed investigating if a possible illegal event occurred that could pose serious environmental risks due to the possibility of dispersing this exotic pathogen in Brazil.…”

Section: Methodsmentioning

confidence: 99%

Using palynological evidence from royal jelly to mediate the spread of Paenibacillus larvae in Brazil

Luz

Guimarães-Cestaro

Serrão

et al. 2018

Hoehnea

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Royal jelly may contain pollen grains and their presence can be used to determine the phytogeographical origin of the product. This study analyzed the phytogeographical origin of commercial royal jelly samples from São Paulo State, tested as part of the Brazilian Federal Inspection System (SIF), found to be contaminated with spores of the bacterium Paenibacillus larvae, that causes the American Foulbrood Disease. The pollen grains of Castanea had the highest total percentage, with lower percentages of Cirsium/Carduus, Cistus, Parthenocissus, Prunus, Quercus, Robinia, Scrophulariaceae, Taraxacum, Tilia, among others. This pollen spectrum is incompatible with royal jelly samples produced in Brazil. The pollen spectrum resembled that of an imported product, compatible with the Northern Hemisphere origin. Brazilian legislation does not require the phytogeographic origin of imported bee products to be analyzed by palynological procedures, but it is mandatory to have a certificate issued by the country of origin attesting the absence of pathogens, monitored with the objective of preventing the exotic diseases from entering Brazil. Palynology, therefore, proved to be fundamental in detecting imports of this contaminated batches.

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Secondary pollen types characteristic of Louisiana honeys

Cited by 10 publications

References 3 publications

Melissopalynological Characterization of North Algerian Honeys

Melissopalynological Characterization of North Algerian Honeys

The use of ETOH for the dilution of honey

Using palynological evidence from royal jelly to mediate the spread of Paenibacillus larvae in Brazil

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