No abstract

Honey bees are responsible for pollinating many native and cultivated plant species. These insects can be affected by many pathogens, including fungi and bacteria, both of which can form spores that are easily dispersed within the colony by means of the stored products, among other routes. The objective of this study was to develop a method to detect spores of the honey bee pathogens Nosema apis, Nosema ceranae, Ascosphaera apis and Paenibacillus larvae in samples of honey, bee pollen and royal jelly. The method was standardized for each product individually, and then analyzed by monoplex and multiplex PCR, which showed the same detection thresholds: 1.25 spores/mL of honey for N. ceranae; 7.5 spores/mL of honey for A. apis; and 0.4 spore/mL of honey for P. larvae, respectively. The standardized technique was effective and rapid for the detection of these pathogens in bee products and can be used for the establishment of official methods of sanitary control of bee products, considering the growing national and international trade of these products and the movement or migration of colonies between regions.

No abstract

Nosemosis is an important disease that affects honey bees (Apis mellifera Lineu), caused by obligate intracellular parasites, Nosema  apis  and/or  Nosema  ceranae. Since the initial detection of N. ceranae in A. mellifera coincided with recent large-scale losses of bee colonies worldwide, the impacts of this parasite under field conditions are of great interest. Here we test two hypotheses, the first one, whether the climatic variables (temperature, air humidity and precipitation) influence the intensity of infection of the microsporidium Nosema spp. in Africanized honey bees (Apis mellifera), and the second, whether the local of hive installation (outdoor or roofed) influences the intensity of infection of these spores in Africanized honey bees. Between August 2013 and August 2016, samples of Africanized bees were collected weekly from 20 colonies, of which ten were located in an open area (outdoor apiary) and ten under a roof on a concrete floor (roofed apiary). N.  ceranae was the only species present. The type of apiary did not influence (p > 0.05) the number of spores of N. ceranae in Africanized bees. However, the infection intensities of the roofed apiary colonies were lower in the autumn. Regarding the meteorological parameters, there was a negative correlation between the winter infection intensities and the minimum temperature in the roofed apiary and the humidity in the outdoor apiary. The highest infection intensities occurred in both apiaries in the spring and summer, which may be related to higher pollen production. On average, the infection intensity was 16.19 ± 15.81 x 105, ranging from zero to 100.5 x105, and there were no records of collapse during the three years.

No abstract

Royal jelly may contain pollen grains and their presence can be used to determine the phytogeographical origin of the product. This study analyzed the phytogeographical origin of commercial royal jelly samples from São Paulo State, tested as part of the Brazilian Federal Inspection System (SIF), found to be contaminated with spores of the bacterium Paenibacillus larvae, that causes the American Foulbrood Disease. The pollen grains of Castanea had the highest total percentage, with lower percentages of Cirsium/Carduus, Cistus, Parthenocissus, Prunus, Quercus, Robinia, Scrophulariaceae, Taraxacum, Tilia, among others. This pollen spectrum is incompatible with royal jelly samples produced in Brazil. The pollen spectrum resembled that of an imported product, compatible with the Northern Hemisphere origin. Brazilian legislation does not require the phytogeographic origin of imported bee products to be analyzed by palynological procedures, but it is mandatory to have a certificate issued by the country of origin attesting the absence of pathogens, monitored with the objective of preventing the exotic diseases from entering Brazil. Palynology, therefore, proved to be fundamental in detecting imports of this contaminated batches.