Secondary Metabolite Production in Callus Cultures of &lt;i&gt;Stevia rebaudiana&lt;/i&gt; Bertoni

Janarthanam, B.; Gopalakrishnan, M.; Sekar, T.

doi:10.3329/bjsir.v45i3.6532

Cited by 30 publications

(12 citation statements)

References 15 publications

(2 reference statements)

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“…Therefore, it can be concluded that in vitro culture is more suitable for the purpose of mucilage production. These findings are in synchrony with those of Janarthanam et al [18] and Rajkumar et al [19], who have obtained large amount of bioactive compound in undifferentiated callus culture than field grown plants. Similar studies have been conducted in Solanum nigrum [20], wherein the authors have reported that the solasodine content from the in vitro callus extracts was much higher than extracts of field grown leaves.…”

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confidence: 94%

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Gupta

Kour

Kaul

et al. 2014

Natl. Acad. Sci. Lett.

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The seed husk of Plantago ovata known as psyllium (Isabgol) yields medicinally important mucilage. The amount of mucilage produced is about 25 % (by weight) of the total seed yield. In the present study, an attempt was made to increase the amount of mucilage through callus cultures of P. ovata. The first step involved establishment of callus cultures in P. ovata. Leaf explants from 10 to 20 day old seedlings were cultured on MS basal medium supplemented with various concentrations of different plant growth regulators. The highest rate of callus induction (89 %) was obtained on MS medium containing 0.5 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg l -1 Kinetin. The mucilage content was estimated from the callus obtained in different media. The best mucilage production was obtained in the MS medium supplemented with 0.25 mg l -1 2,4-D and 0.25 mg l -1 thidiazuron. Significant differences with regard to the total mucilage content were recorded. Overall, the callus produced nearly five times more mucilage than the seeds. The present technology provides an alternative route to production of large quantities of mucilage without plants.The seed husk of Plantago ovata Forsk, is an effective laxative. Seeds of P. ovata release mucilage in water, which is an anionic polysaccharide of L-arabinose, D-xylose and Dgalacturonic acid [1], and is used to treat diverticulitis [2], constipation, diarrhoea, haemorrhoids, bladder problems, irritable bowel syndrome, high blood pressure, high cholesterol and type 2 diabetes [3]. Mucilage of plant origin is in high demand nowadays, and it is very difficult to fulfil the entire demand by field grown plants only. For this purpose, a striking and very promising alternative system for commercial exploitation is the plant tissue culture by using cell suspension culture systems [4]. However, so far any attempts to increase mucilage have not yielded positive results in P. ovata. Studies along these lines have been conducted in Plantago lanceolata where in media having different combinations of plant growth regulators (PGRs) have been used to obtain mucilage from callus [5]. It has been reported that callus cultures are relatively rich in mucilage which commonly make up between 8-10 and 0.2-3.7 % of dry weight in some plants [6,7]. To the best of our knowledge, there are no reports on in vitro mucilage production from the callus cultures of P. ovata. Therefore, the present study was aimed to explore the possibility of mucilage production through in vitro culture.Seeds of P. ovata were available in the collection center of School of Biotechnology, University of Jammu, Jammu, India. The seeds were sterilized first with 70 % V/V ethanol for 1 min, then with 5 % w/v sodium-hypochlorite solution for 20 min, finally washed five times with sterile distilled water. The sterilized seeds were aseptically germinated on an agar-solidified MS basal medium [8] and incubated at a temperature of 25 ± 1°C. Leaf segments (1.5 cm) were excised from 10 to 20 day-old seedlings and were used as explants ...

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supporting

confidence: 94%

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Gupta

Kour

Kaul

et al. 2014

Natl. Acad. Sci. Lett.

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“…Similar results have been reported for the increased extraction of solasodine in Solanum nigrum (Yogananth et al, 2009), taxol in Taxus baccata (Khosroushahi et al, 2005), artemisinin in artemisia annua (Pu et al, 85 2009), nitidine in Toddalia asiatica (Rajkumar et al, 2010), stevioside in stevia rebaudiana (Janarthanam et al, 2010), ajmalicine and indole alkaloide in Catharanthus roseus (Zhao et al, 2001;Namdeo, 2002), jaceosidin and syringin in Saussurea medusa (Yu et al, 2006), and artemisinin in Artemisia annua (Pu et al, 2009) from in vitro cell cultures, all of which were much higher than the extracts obtained from field grown plants. The chemical structures of secondary metabolites are usually complex and their production is costly, but plant cell tissue cultures could help overcome these limitations.…”

Section: Genotypic Assaysupporting

confidence: 73%

Mucilage synthesis by in vitro cell culture in different species of Alyssum

Afshar¹,

Golkar²

2016

bta

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Mucilage is a class of polysaccharides found in some plants that have pharmaceutical effects as anti-hemorrhoids.Alyssum is one such species; its seeds produce mucilage that possesses pharmaceutical properties. The aim of this study was to optimize the conditions for callus production and mucilage synthesis in Alyssum species in a tissue culture procedure. In the study presented here, callus initiation in different genotypes of Alyssum species (A. inflatum, A. lepidium, and A. strigosum) has been investigated for the first time. Different combinations of 2,4 Dichlorophenoxy acetic acid (2,4 D), Kinetin (Kin) and Benzyl amino purin (BAP) were used to optimize callus initiation frequency and callus growth rates (CGR) in hypocotyl explants. The highest rates of callus induction (%) and callus growth rates (CGR) were achieved with 2.5 mg @ l Alyssum sp. using in vitro cell culture.

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“…were founded inter-nodal segments initiated callus formation earlier than node and leaf explants [20]. Leaf explants showed better callus initiation than nodal explants [21]. Different explants including leaf, nodal and internodal segments explants on different concentrations of 2,4-D (2, 3, 4 and 5 mg/l) in MS medium assayed by Uddin et al (2006) that callus induction was observed from MS medium with 3.0 mg/l 2,4-D (2,4 Dichlorophenoxy acetic acid) [22].…”

Section: Tissue Culturementioning

confidence: 98%

Biotechnological approaches in Stevia rebaudiana and its Therapeutic Applications

Karimi¹

2017

Adv. Biomed. Pharma

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Stevia rebaudiana (Bertoni) is a high value and economic medicinal plant because of its pharmaceutically active compounds throughout the world. Stevia acts as a non-caloric natural-source with therapeutic applications including anti-cariogenic, antimicrobial, anticancer, antioxidant and scavenge free radical and antidiabetic properties. Biotechnological techniques offer novel approaches to the industrial production, propagation, conservation and manipulation of Stevia. The aim of this paper is to describe Stevia rebaudiana (Bertoni) biotechnological approaches and its application in foods and medicine.

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Secondary Metabolite Production in Callus Cultures of <i>Stevia rebaudiana</i> Bertoni

Cited by 30 publications

References 15 publications

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Mucilage synthesis by in vitro cell culture in different species of Alyssum

Biotechnological approaches in Stevia rebaudiana and its Therapeutic Applications

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