Seborrheic keratosis of conjunctiva simulating a malignant melanomaan immunocytochemical study with impression cytology

Tseng, Sung Huei; Chen, Ying Ting; Huang, Fu Chin; Jin, Ying

doi:10.1016/s0161-6420(99)90446-3

Cited by 25 publications

(21 citation statements)

References 18 publications

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“…4 This technique was later used in the preoperative diagnosis of seborrhoeic keratosis of the conjunctiva simulating a malignant melanoma. 17 Thiel et al used a biopore membrane device to collect ocular surface specimens and apply immunopathological methods to diagnose superficial viral infections like herpes simplex virus, varicella zoster virus, and adenovirus. 5 More recently impression cytology has been used to demonstrate conjunctival metaplasia as a result of the use of topical antiglaucoma drugs.…”

Section: Microscopy and Clinical Applicationsmentioning

confidence: 99%

Impression cytology of the ocular surface

Singh

Joseph²,

Umapathy³

et al. 2005

British Journal of Ophthalmology

180

110

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Impression cytology refers to the application of a cellulose acetate filter to the ocular surface to remove the superficial layers of the ocular surface epithelium. These cells can then be subjected to histological, immunohistological, or molecular analysis. Proper technique is essential as the number of cells sampled can vary considerably. Generally two to three layers of cells are removed in one application but deeper cells can be accessed by repeat application over the same site. Applications for impression cytology include diagnosing a wide range of ocular surface disorders, documenting sequential changes in the conjunctival and corneal surface over time, staging conjunctival squamous metaplasia, and monitoring effects of treatment. It is also a useful investigational tool for analysing ocular surface disease with immunostaining and DNA analysis. It is non-invasive, relatively easy to perform, and yields reliable information about the area sampled with minimal discomfort to the patient. Major ophthalmic centres should develop and introduce this technique into routine clinical practice. This is best achieved with a team approach including the ophthalmologist, pathologist, microbiologist, and the immunologist.

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Section: Microscopy and Clinical Applicationsmentioning

confidence: 99%

Impression cytology of the ocular surface

Singh

Joseph²,

Umapathy³

et al. 2005

British Journal of Ophthalmology

180

110

View full text Add to dashboard Cite

show abstract

“…One of these changes is a pathological transition to a keratinized non-secretory corneal and conjunctival surface, characterized by goblet cell depletion. 3,4 A range of methods have been applied to support the dry eye syndrome diagnosis, including the Ocular Surface Disease Index (OSDI) survey, comprising 12 questions on ocular surface symptomatology divided into three categories (ocular discomfort, functionality and effect of environmental conditions). 5 The survey provides an estimation of dry eye severity degree.…”

Section: Introductionmentioning

confidence: 99%

Conjunctival impression cytology in patients with normal and impaired OSDI scores

Zuazo

López-Ponce

Salinas-Toro

et al. 2014

Archivos de la Sociedad Española de Oftalmología (English Editi

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“…IC disclosed basaloid cells intermixing with squamoid cells, and these cells demonstrated positive immunoreactivity to cytokeratin and no reactivity to HMB-45 and therefore were proven to represent an epithelium-derived tumor despite of being pigmented. This report illustrated that IC combined with immunocytochemical staining may be a valuable diagnostic aid in the differentiation of pigmented conjunctival tumors prior to treatment (15) . The published correlation rate with IC for predicting the subsequent histological findings ranged between 77% (55/71) and 80% (20/25), and both cellulose acetate (16) and Biopore membranes (17) have been successfully used.…”

Section: Application Of Ic In the Evaluation Of Lesions Of Epithelialmentioning

confidence: 85%

“…This combination of IC and immunocytochemistry was first described by Krenzer and Freddo in normal human conjunctiva in 1997, enabling the simultaneous evaluation of IC specimens for immunoreactivity to cytokeratin and morphological details (45) . Nevertheless, as indicated in this review, there was only a single case (15) using this combined technique in the evaluation of an ocular surface tumor. Thus, the sensitivity and reliability of IC combined with immunocytochemical staining in the differentiation of ocular surface tumors need further evaluation in large-scale studies.…”

mentioning

confidence: 93%

Impression cytology in the evaluation of ocular surface tumors: review article

Barros¹,

Almeida²,

Lowen³

et al. 2015

Arquivos Brasileiros De Oftalmologia

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Impression cytology (IC) has been widely used as a method for evaluating the ocular surface and superficial cells layers in the diagnosis and follow-up after treatment of several ocular surface tumors of both epithelial and melanocytic origin. Information regarding this can be found in the English-language literature since 1992. Using either cellulose acetate or Biopore membranes for specimen collection, a high correlation has been found between IC and tissue histology. Compared with exfoliative cytology with spatula, IC is less traumatic to the patient's eye, provides a precise location of the area being studied, and allows accurate observation of the cells the way they exist in vivo. The additional advantage of IC is the preservation of limbal stem cells responsible for continuous corneal epithelium renewal; these can be affected after incisional or excisional biopsy at the corneoscleral limbus, which is the most frequent site of appearance of tumors in the stratified epithelium. Treatment for ocular surface squamous neoplasia has historically included surgery, but nonsurgical interventions have also been adopted. Hence, in certain cases, ophthalmologists may prefer interventions less invasive than surgical biopsy such as of impression cytology for both initial diagnosis and therapeutic monitoring of treatment for ocular surface lesions. Nevertheless, it should be considered that IC may be less helpful if the results conflict with the clinical picture or if the clinical diagnosis is uncertain and results are negative. In such cases, surgical biopsy is required for accurate diagnosis. The purpose of this review is to examine the published literature on the utilization of IC for the diagnosis and management of ocular surface tumors and to discuss the requirement for further investigation on the subject.

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Seborrheic keratosis of conjunctiva simulating a malignant melanomaan immunocytochemical study with impression cytology

Cited by 25 publications

References 18 publications

Impression cytology of the ocular surface

Impression cytology of the ocular surface

Conjunctival impression cytology in patients with normal and impaired OSDI scores

Impression cytology in the evaluation of ocular surface tumors: review article

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