Impression cytology of the ocular surface

Singh, Rashmi; Joseph, Annie; Umapathy, T; Tint, Naing L.; Dua, Hs

doi:10.1136/bjo.2005.073916

Cited by 180 publications

(132 citation statements)

References 32 publications

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“…On the other hand, impression cytology and brush cytology are widely used methods to evaluate the ocular surface pathologic changes. 7 They are noninvasive, repeatable, and useful in following the changes in the ocular surface. 8,9 However, there are few reports on impression cytology changes and brush cytology characteristics in patients with cGVHD-related dry eye.…”

Section: Introductionmentioning

confidence: 99%

Baseline profiles of ocular surface and tear dynamics after allogeneic hematopoietic stem cell transplantation in patients with or without chronic GVHD-related dry eye

Wang

Ogawa

Doğru

et al. 2009

Bone Marrow Transplant

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We evaluated ocular surface alterations in allogeneic hematopoietic stem cell transplantation (HSCT) recipients with or without chronic GVHD-related dry eye in a prospective study. Fifty eyes of 25 post-HSCT patients and 28 eyes of 14 age-matched healthy controls were included. Meibomian gland (MG) obstruction, tear evaporation rate, corneal sensitivity (CS), Schirmer test-I, tear break-up time (BUT) and ocular surface vital staining were examined. Conjunctival impression and brush cytology specimens were collected to evaluate the goblet cell density (GCD) and the inflammatory cell numbers. Obvious MG obstruction, decreased CS and enhanced tear evaporation rate were found in post-HSCT patients compared with normal controls. In addition, decreased conjunctival GCD, increased conjunctival squamous metaplasia and inflammatory cells were noted in cGVHD-related dry eyes compared with normal controls and post-HSCT without dry eye subjects. Furthermore, the conjunctival inflammatory cells were significantly higher in severe dry eyes compared with mild dry eyes (P ¼ 0.03). We found comprehensive ocular surface alteration in post-HSCT patients, regardless of whether they had cGVHD-related dry eye or not. The results suggest that the extent of inflammatory process seems to have a pivotal role in the outcome of the cGVHD-related dry eye.

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Section: Introductionmentioning

confidence: 99%

Baseline profiles of ocular surface and tear dynamics after allogeneic hematopoietic stem cell transplantation in patients with or without chronic GVHD-related dry eye

Wang

Ogawa

Doğru

et al. 2009

Bone Marrow Transplant

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“…Biopore, however, may sample only the first layer of cells on the conjunctiva, unless it is repeated several times to acquire cells of deeper layers (9) . Similarly, IC with cellulose acetate filters was able to sample deeper layers when performed repeatedly (10) . A case of a patient with an irregular pigmented lesion of the lower eyelid margin simulating malignant tumor, which was treated based on the results of IC and diagnosed with secondary melanosis by histology, was presented in 2009.…”

Section: Application Of Ic In the Evaluation Of Lesions Of Melanocytimentioning

confidence: 99%

Impression cytology in the evaluation of ocular surface tumors: review article

Barros¹,

Almeida²,

Lowen³

et al. 2015

Arquivos Brasileiros De Oftalmologia

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Impression cytology (IC) has been widely used as a method for evaluating the ocular surface and superficial cells layers in the diagnosis and follow-up after treatment of several ocular surface tumors of both epithelial and melanocytic origin. Information regarding this can be found in the English-language literature since 1992. Using either cellulose acetate or Biopore membranes for specimen collection, a high correlation has been found between IC and tissue histology. Compared with exfoliative cytology with spatula, IC is less traumatic to the patient's eye, provides a precise location of the area being studied, and allows accurate observation of the cells the way they exist in vivo. The additional advantage of IC is the preservation of limbal stem cells responsible for continuous corneal epithelium renewal; these can be affected after incisional or excisional biopsy at the corneoscleral limbus, which is the most frequent site of appearance of tumors in the stratified epithelium. Treatment for ocular surface squamous neoplasia has historically included surgery, but nonsurgical interventions have also been adopted. Hence, in certain cases, ophthalmologists may prefer interventions less invasive than surgical biopsy such as of impression cytology for both initial diagnosis and therapeutic monitoring of treatment for ocular surface lesions. Nevertheless, it should be considered that IC may be less helpful if the results conflict with the clinical picture or if the clinical diagnosis is uncertain and results are negative. In such cases, surgical biopsy is required for accurate diagnosis. The purpose of this review is to examine the published literature on the utilization of IC for the diagnosis and management of ocular surface tumors and to discuss the requirement for further investigation on the subject.

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“…Histological and morphological analysis of conjunctive cells samples, isolated by CIC and ICSEM, was performed using the methodology reported in literature [8][9][10][11] …”

Section: Methodsmentioning

confidence: 99%

“…CIC is a noninvasive method of conjunctival biopsy which allows the examination of conjunctival changes at the cellular level, the etiology of various ocular surface disorders, documenting on age changes of ocular surface, monitoring the effects of the treatment of dry eye, staining of squamous metaplasia, and it contribute to the study of ocular surface diseases with immune histochemical and DNA analysis [7][8][9][10].…”

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confidence: 99%