Seasonal variations in the composition of ram seminal plasma and its effect on frozen-thawed ram sperm

Domínguez, Marcelo Pérez; Falcinelli, A.; Hozbor, F.; Sánchez, Edith; Cesari, Andreína; Alberio, R. H.

doi:10.1016/j.theriogenology.2007.11.010

Cited by 68 publications

(55 citation statements)

References 45 publications

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“…Roles of SP on mature spermatozoa have been widely studied with contrasting results, and research in several species highlight the varying roles for SP, including 1) activation and augmentation of the motility of spermatozoa; 2) buffering to provide the optimal osmotic and nutrient medium; 3) prevention of premature activation during physiological transport of spermatozoa and stabilization of the plasma membrane (PM) with capacitation inhibitors (Desnoyers and Manjunath, 1992; Villemure et al, 2003); 4) protection of spermatozoa from phagocytosis and destruction in an inflammatory environment in the horse (Alghamdi et al, 2004); 5) regulation of sperm transport and elimination (Troedsson et al, 2005); 6) hastening of ovulation in cows (Marion, 1950) and induction of ovulation in pigs and camelids (O'Leary et al, 2004; Ratto et al, 2005); 7) assistance in sperm‐ovum interactions (Souza et al, 2008); 8) activation of the expression of embryotrophic cytokines and help toward preparing the maternal tract for the developing embryo, particularly by facilitating immune changes required to accommodate pregnancy (Robertson, 2005); and 9) influence over fertility (Rozeboom et al, 2000). Moreover, the addition of SP or its components into postthawed semen increases oxygen uptake and motility of spermatozoa (White et al, 1987), helps to recover some surface proteins (Domínguez et al, 2008), reverts cryodamage on PM of ram spermatozoa (Rebolledo et al, 2007), and increases overall sperm quality parameters (Maxwell et al, 2007). Conversely, the detrimental effects of SP on motility and viability of spermatozoa after freeze‐thawing have also been reported (Garcia and Graham, 1987).…”

Section: Biochemical Constituents Of Sp and Their Functionsmentioning

confidence: 99%

Seminal Plasma: An Essential Attribute to Spermatozoa

Juyena¹,

Stelletta²

2012

Journal of Andrology

221

169

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The biochemical composition of seminal plasma (SP) is very complex and variable among species. Advances in reproductive technologies reveal SP as a nutritive-protective medium for sperm cells suspended in it, and some SP components are very important for sperm metabolism, as well as sperm function, survival, and transport in the female reproductive tract. Biochemistry of SP is a relatively modern but rapidly expanding field of research, particularly concerning the biological significance of the various biochemical constituents of SP. However, identification of active factors in SP and mechanisms by which they act are not sufficiently understood in different species. Especially in camelids, the origin, composition, and function of the viscous component of SP remain a mystery, and only some biochemical and morphological characteristics of SP have been described. In this review, we focus on the current understanding of physiological and biochemical properties of SP in ruminants and camelids.

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Section: Biochemical Constituents Of Sp and Their Functionsmentioning

confidence: 99%

Seminal Plasma: An Essential Attribute to Spermatozoa

Juyena¹,

Stelletta²

2012

Journal of Andrology

221

169

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“…In paticular, ram sperm are more sensitive to freezing than gametes from other mammalian species, and because of the reduced motility of frozen-thawed ram sperm, the source of sperm used for cervical AI is restricted to fresh semen, whereas frozen-thawed semen can be used only for intrauterine insemination [14]. The nature of the cervical morphology of the ewe causes a low fertility (20-30%) in ewes inseminated with frozen semen into the cervical orifice, an ordinal deposition site in the sheep, and AI has not been fully applied in the field, except the intrauterine AI using laparoscopy (60-80% lambing rates) [5,[15][16][17][18] In Norway, Paulenz et al [19,20] inseminated frozen ram semen into either the cervical orifice or deep vagina and obtained a 70% lambing rate.…”

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confidence: 99%

Fertility after Different Artificial Insemination Methods Using a Synthetic Semen Extender in Sheep

Hiwasa

Kohno

Togari

et al. 2009

Journal of Reproduction and Development

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Abstract. The present study aimed to investigate the fertility of ewes artificially inseminated with three different methods using a synthetic semen extender, AndroMed. The three methods of artificial insemination (AI) were cervical AI with fresh-diluted or frozen-diluted semen at observed estrus, and an intrauterine AI with frozen-thawed semen. A total of 80 ewes were treated with a controlled internal drug release (CIDR) containing 0.3 g progesterone per device for 12 days. In Experiment 1 (26 Suffolk ewes), superovulation was induced with 20 mg follicle-stimulating hormone and 250 IU equine chorionic gonadotropin (eCG) two days and one day before CIDR removal, respectively, during the nonbreeding season. In Experiment 2 (54 Suffolk and Suffolk crossbred ewes), an intramuscular injection of 500 IU eCG was administered one day before CIDR removal to synchronize estrus and ovulation during the breeding season. In Experiment 1, fresh-diluted or frozen-thawed semen was deposited into the cervical orifice after estrus detection, and an intrauterine AI with frozen-thawed semen was performed by laparoscopy at a fixed-time basis without estrus detection. Embryos were recovered by uterine flushing 6 days after AI, and the rates of recovered, fertilized (cleaved) ova and embryos at the morula or blastocyst stage were compared among the three AI methods. In Experiment 2, the pregnancy rates after the three AI methods were compared. In Experiment 1, the rates of recovered ova were not significantly different among the three AI methods (52.5-56.7%). The rate of fertilized ova (81.0%) by laparoscopic AI with frozen-thawed semen was significantly higher compared with cervical AI of fresh-diluted (25.5%) or frozenthawed (3.5%) semen, but the rate of embryos at the morula or blastocyst stage (17.6%) was significantly lower than that of the cervical AI with fresh-diluted semen (69.2%). The rates of ewes yielding fertilized ova were not significantly different among the three groups (44.4, 11.1 and 62.5% for cervical AI with fresh-diluted and frozen-thawed semen and intrauterine AI with frozen-thawed semen). In Experiment 2, the pregnancy rate of ewes intrauterinally inseminated with frozen-thawed semen (72.2%) was significantly higher than those of ewes inseminated cervically with freshdiluted (5.5%) or frozen-thawed (0.0%) semen. The present results showed that acceptable fertilization and pregnancy rates could be obtained by an intrauterine AI with frozen-thawed semen using a synthetic semen extender (AndroMed), but not sufficient by the cervical AI with either fresh or frozen semen. Key words: Artificial insemination (AI), Fertility, Semen extender, Sheep (J. Reprod. Dev. 55: [50][51][52][53][54] 2009) n artificial insemination (AI) of farm animals including sheep, egg yolk or milk based semen extender have been widely utilized for semen cryopreservation [1][2][3]. Our pervious studies [4,5] reported that bovine serum albumin (BSA) could be used instead of egg yolk as a semen extender. However, egg yolk and BSA are of ani...

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“…Similarly, no beneficial results of SP supplementation were reported in chilled ram sperm (Morrier, Castonguay, & Bailey, ). Moreover, the study in cryopreserved ram sperm found no effect on sperm motility (Domínguez et al., ) or no improvement of fertility (O'Meara et al., ) when adding SP in extender.…”

Section: Discussionmentioning

confidence: 98%

“…The effect of SP on diluted semen could vary depending on the source of SP and has been mentioned for bull (Garner et al., ), boar (Caballero et al., ) and ram (Domínguez et al., ). There was no effect of homologous SP, compared to autologous SP in chilled elephant sperm quality (Pinyopummin et al., ).…”

Section: Discussionmentioning

confidence: 99%

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

Pinyopummin

Mahasawangkul²,

Nunklang

et al. 2018

Reprod Domestic Animals

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The dilution effect and effect of restoring seminal plasma (SP) proportion in diluted semen were determined in chilled Asian elephant sperm. Semen was collected from eight males, and samples with ≥30% motile sperm were used in the study. Tris-glucose-egg yolk extender (TE) was used for cooled storage at 4°C for 48 hr. In experiment 1 (n = 18), semen was diluted to 1:1, 1:3, 1:7 and 1:15 with TE (volume per volume). There were no significant changes in sperm viability and sperm with normal acrosome integrity among dilutions, but sperm motility and motility velocities were greater (p < .05) in the 1:1 dilution than those of the 1:7 and 1:15 dilutions at 48 hr of storage. In experiment 2, supplemented SP was derived from elephants and stallions. In experiment 2.1, diluted semen (1:7 dilution) was restored with SP to obtain a 1:2 proportion (n = 8). Sperm motility, viability and sperm with normal acrosome integrity were similar among treatments, but motility velocities were greater (p < .05) with stallion SP at 48 hr of storage. In experiment 2.2, diluted semen (1:15 dilution) was restored with SP to obtain a 1:3 proportion (n = 10). Sperm viability and sperm with normal acrosome integrity were similar among treatments at 48 hr of storage. However, sperm motility and motility velocities were greater (p < .05) with stallion SP than those of others. In conclusion, elephant sperm motility was affected by a dilution effect and restoration of SP proportion with stallion SP, but not with elephant SP, could improve motility in chilled highly diluted sperm.

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Seasonal variations in the composition of ram seminal plasma and its effect on frozen-thawed ram sperm

Cited by 68 publications

References 45 publications

Seminal Plasma: An Essential Attribute to Spermatozoa

Seminal Plasma: An Essential Attribute to Spermatozoa

Fertility after Different Artificial Insemination Methods Using a Synthetic Semen Extender in Sheep

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm

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